Experiment: ICP1_phage_2.3_MOI_rep1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_17265 and CSW01_17270 are separated by 4 nucleotides CSW01_17270 and CSW01_17275 are separated by 270 nucleotides CSW01_17275 and CSW01_17280 are separated by 230 nucleotides
CSW01_17265: CSW01_17265 - hypothetical protein, at 544,511 to 544,693
_17265
CSW01_17270: CSW01_17270 - isoprenoid biosynthesis protein ElbB, at 544,698 to 545,348
_17270
CSW01_17275: CSW01_17275 - large conductance mechanosensitive channel protein MscL, at 545,619 to 546,029
_17275
CSW01_17280: CSW01_17280 - formate--tetrahydrofolate ligase, at 546,260 to 548,008
_17280
Position (kb)
545
546
547 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 544.629 kb on - strand, within CSW01_17265 at 544.659 kb on + strand, within CSW01_17265 at 544.659 kb on + strand, within CSW01_17265 at 544.667 kb on - strand, within CSW01_17265 at 544.734 kb on + strand at 544.734 kb on + strand at 544.746 kb on + strand at 544.751 kb on + strand at 544.810 kb on + strand, within CSW01_17270 at 544.810 kb on + strand, within CSW01_17270 at 544.810 kb on + strand, within CSW01_17270 at 544.824 kb on + strand, within CSW01_17270 at 544.857 kb on + strand, within CSW01_17270 at 544.857 kb on + strand, within CSW01_17270 at 544.857 kb on + strand, within CSW01_17270 at 544.865 kb on - strand, within CSW01_17270 at 545.027 kb on + strand, within CSW01_17270 at 545.027 kb on + strand, within CSW01_17270 at 545.027 kb on + strand, within CSW01_17270 at 545.027 kb on + strand, within CSW01_17270 at 545.027 kb on + strand, within CSW01_17270 at 545.032 kb on + strand, within CSW01_17270 at 545.035 kb on - strand, within CSW01_17270 at 545.035 kb on - strand, within CSW01_17270 at 545.035 kb on - strand, within CSW01_17270 at 545.035 kb on - strand, within CSW01_17270 at 545.035 kb on - strand, within CSW01_17270 at 545.035 kb on - strand, within CSW01_17270 at 545.035 kb on - strand, within CSW01_17270 at 545.035 kb on - strand, within CSW01_17270 at 545.091 kb on + strand, within CSW01_17270 at 545.099 kb on - strand, within CSW01_17270 at 545.099 kb on - strand, within CSW01_17270 at 545.101 kb on + strand, within CSW01_17270 at 545.101 kb on + strand, within CSW01_17270 at 545.171 kb on - strand, within CSW01_17270 at 545.205 kb on + strand, within CSW01_17270 at 545.220 kb on + strand, within CSW01_17270 at 545.220 kb on + strand, within CSW01_17270 at 545.228 kb on - strand, within CSW01_17270 at 545.381 kb on + strand at 545.389 kb on - strand at 545.410 kb on + strand at 545.415 kb on + strand at 545.415 kb on + strand at 545.418 kb on - strand at 545.561 kb on - strand at 545.636 kb on + strand at 545.935 kb on - strand, within CSW01_17275 at 546.261 kb on - strand at 546.263 kb on - strand at 546.364 kb on + strand at 546.385 kb on + strand at 546.390 kb on + strand at 546.393 kb on - strand at 546.398 kb on - strand at 546.494 kb on + strand, within CSW01_17280 at 546.494 kb on + strand, within CSW01_17280 at 546.500 kb on + strand, within CSW01_17280 at 546.544 kb on + strand, within CSW01_17280 at 546.552 kb on - strand, within CSW01_17280 at 546.553 kb on - strand, within CSW01_17280 at 546.753 kb on + strand, within CSW01_17280 at 546.887 kb on + strand, within CSW01_17280 at 546.936 kb on + strand, within CSW01_17280 at 546.936 kb on + strand, within CSW01_17280 at 546.982 kb on + strand, within CSW01_17280 at 546.989 kb on + strand, within CSW01_17280
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_2.3_MOI_rep1 remove 544,629 - CSW01_17265 0.64 -2.2 544,659 + CSW01_17265 0.81 -3.3 544,659 + CSW01_17265 0.81 -1.1 544,667 - CSW01_17265 0.85 -3.6 544,734 + -1.4 544,734 + -2.1 544,746 + +0.4 544,751 + -4.0 544,810 + CSW01_17270 0.17 +0.2 544,810 + CSW01_17270 0.17 -0.1 544,810 + CSW01_17270 0.17 +1.8 544,824 + CSW01_17270 0.19 -1.0 544,857 + CSW01_17270 0.24 +0.8 544,857 + CSW01_17270 0.24 +0.9 544,857 + CSW01_17270 0.24 +1.6 544,865 - CSW01_17270 0.26 +0.6 545,027 + CSW01_17270 0.51 -1.8 545,027 + CSW01_17270 0.51 +1.2 545,027 + CSW01_17270 0.51 +1.0 545,027 + CSW01_17270 0.51 +0.4 545,027 + CSW01_17270 0.51 -1.6 545,032 + CSW01_17270 0.51 +0.1 545,035 - CSW01_17270 0.52 +0.6 545,035 - CSW01_17270 0.52 -2.1 545,035 - CSW01_17270 0.52 +0.4 545,035 - CSW01_17270 0.52 -0.2 545,035 - CSW01_17270 0.52 -2.0 545,035 - CSW01_17270 0.52 +0.8 545,035 - CSW01_17270 0.52 -0.0 545,035 - CSW01_17270 0.52 +1.8 545,091 + CSW01_17270 0.60 -1.4 545,099 - CSW01_17270 0.62 +0.8 545,099 - CSW01_17270 0.62 +1.9 545,101 + CSW01_17270 0.62 +0.7 545,101 + CSW01_17270 0.62 +0.7 545,171 - CSW01_17270 0.73 -1.3 545,205 + CSW01_17270 0.78 +1.0 545,220 + CSW01_17270 0.80 +1.3 545,220 + CSW01_17270 0.80 -2.2 545,228 - CSW01_17270 0.81 +0.5 545,381 + +1.0 545,389 - +0.9 545,410 + -1.5 545,415 + +1.9 545,415 + -0.9 545,418 - -2.5 545,561 - +1.1 545,636 + +0.7 545,935 - CSW01_17275 0.77 -2.0 546,261 - +0.4 546,263 - -2.4 546,364 + -0.9 546,385 + -1.7 546,390 + -0.1 546,393 - -2.8 546,398 - +0.9 546,494 + CSW01_17280 0.13 -1.2 546,494 + CSW01_17280 0.13 +1.4 546,500 + CSW01_17280 0.14 -2.7 546,544 + CSW01_17280 0.16 +1.1 546,552 - CSW01_17280 0.17 -2.2 546,553 - CSW01_17280 0.17 -2.1 546,753 + CSW01_17280 0.28 +0.4 546,887 + CSW01_17280 0.36 -0.5 546,936 + CSW01_17280 0.39 -0.3 546,936 + CSW01_17280 0.39 +1.0 546,982 + CSW01_17280 0.41 -2.6 546,989 + CSW01_17280 0.42 +0.4
Or see this region's nucleotide sequence