Strain Fitness in Vibrio cholerae E7946 ATCC 55056 around CSW01_10480

Experiment: ICP1_phage_2.3_MOI_rep1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCSW01_10470 and CSW01_10475 are separated by 108 nucleotidesCSW01_10475 and CSW01_10480 are separated by 92 nucleotidesCSW01_10480 and CSW01_10485 are separated by 95 nucleotidesCSW01_10485 and CSW01_10490 are separated by 48 nucleotidesCSW01_10490 and CSW01_10495 are separated by 73 nucleotides CSW01_10470: CSW01_10470 - phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent), at 2,254,920 to 2,256,566 _10470 CSW01_10475: CSW01_10475 - replication initiation regulator SeqA, at 2,256,675 to 2,257,208 _10475 CSW01_10480: CSW01_10480 - histidine kinase, at 2,257,301 to 2,258,074 _10480 CSW01_10485: CSW01_10485 - DUF2788 domain-containing protein, at 2,258,170 to 2,258,391 _10485 CSW01_10490: CSW01_10490 - flavodoxin FldA, at 2,258,440 to 2,258,967 _10490 CSW01_10495: CSW01_10495 - LrgB family protein, at 2,259,041 to 2,259,730 _10495 Position (kb) 2257 2258 2259Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 2256.323 kb on + strand, within CSW01_10470at 2256.479 kb on + strandat 2256.555 kb on - strandat 2256.617 kb on + strandat 2256.617 kb on + strandat 2256.617 kb on + strandat 2256.617 kb on + strandat 2256.625 kb on - strandat 2256.625 kb on - strandat 2257.237 kb on - strandat 2257.273 kb on + strandat 2257.281 kb on - strandat 2257.318 kb on - strandat 2257.323 kb on + strandat 2257.325 kb on + strandat 2257.431 kb on + strand, within CSW01_10480at 2257.431 kb on + strand, within CSW01_10480at 2257.431 kb on + strand, within CSW01_10480at 2257.431 kb on + strand, within CSW01_10480at 2257.432 kb on - strand, within CSW01_10480at 2257.439 kb on - strand, within CSW01_10480at 2257.439 kb on - strand, within CSW01_10480at 2257.439 kb on - strand, within CSW01_10480at 2257.439 kb on - strand, within CSW01_10480at 2257.475 kb on - strand, within CSW01_10480at 2257.508 kb on - strand, within CSW01_10480at 2257.508 kb on - strand, within CSW01_10480at 2257.622 kb on + strand, within CSW01_10480at 2257.791 kb on + strand, within CSW01_10480at 2257.796 kb on + strand, within CSW01_10480at 2257.827 kb on - strand, within CSW01_10480at 2258.129 kb on + strandat 2258.129 kb on + strandat 2258.137 kb on - strandat 2258.139 kb on - strandat 2258.366 kb on + strand, within CSW01_10485at 2258.385 kb on + strandat 2258.385 kb on + strandat 2258.385 kb on + strandat 2258.948 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction ICP1_phage_2.3_MOI_rep1
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2,256,323 + CSW01_10470 0.85 +2.8
2,256,479 + +0.5
2,256,555 - +0.5
2,256,617 + +2.3
2,256,617 + -2.8
2,256,617 + -0.5
2,256,617 + +1.1
2,256,625 - -0.6
2,256,625 - +1.7
2,257,237 - -2.5
2,257,273 + +0.6
2,257,281 - +0.6
2,257,318 - +0.5
2,257,323 + -2.6
2,257,325 + -0.2
2,257,431 + CSW01_10480 0.17 -1.6
2,257,431 + CSW01_10480 0.17 +2.1
2,257,431 + CSW01_10480 0.17 -1.4
2,257,431 + CSW01_10480 0.17 +0.9
2,257,432 - CSW01_10480 0.17 +1.2
2,257,439 - CSW01_10480 0.18 +1.4
2,257,439 - CSW01_10480 0.18 +2.0
2,257,439 - CSW01_10480 0.18 -3.9
2,257,439 - CSW01_10480 0.18 +0.1
2,257,475 - CSW01_10480 0.22 -1.5
2,257,508 - CSW01_10480 0.27 -0.2
2,257,508 - CSW01_10480 0.27 -1.7
2,257,622 + CSW01_10480 0.41 +0.4
2,257,791 + CSW01_10480 0.63 -2.3
2,257,796 + CSW01_10480 0.64 +0.9
2,257,827 - CSW01_10480 0.68 -0.2
2,258,129 + +2.5
2,258,129 + +2.8
2,258,137 - -1.1
2,258,139 - -1.4
2,258,366 + CSW01_10485 0.88 +0.6
2,258,385 + -2.4
2,258,385 + -1.6
2,258,385 + +1.5
2,258,948 - +2.1

Or see this region's nucleotide sequence