Experiment: ICP1_phage_2.3_MOI_rep1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_03125 and CSW01_03130 are separated by 63 nucleotides CSW01_03130 and CSW01_03135 are separated by 10 nucleotides CSW01_03135 and CSW01_03140 are separated by 2 nucleotides CSW01_03140 and CSW01_03145 overlap by 4 nucleotides
CSW01_03125: CSW01_03125 - ABC transporter permease, at 616,591 to 617,361
_03125
CSW01_03130: CSW01_03130 - pantoate--beta-alanine ligase, at 617,425 to 618,306
_03130
CSW01_03135: CSW01_03135 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 618,317 to 619,111
_03135
CSW01_03140: CSW01_03140 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase, at 619,114 to 619,617
_03140
CSW01_03145: CSW01_03145 - polynucleotide adenylyltransferase PcnB, at 619,614 to 620,987
_03145
Position (kb) 618
619
620 Strain fitness (log2 ratio) -2
-1
0
1
2 at 617.318 kb on - strand at 617.318 kb on - strand at 617.355 kb on - strand at 617.383 kb on + strand at 617.383 kb on + strand at 619.148 kb on - strand at 619.830 kb on - strand, within CSW01_03145 at 619.918 kb on - strand, within CSW01_03145 at 619.918 kb on - strand, within CSW01_03145 at 619.950 kb on - strand, within CSW01_03145
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_2.3_MOI_rep1 remove 617,318 - +0.6 617,318 - -0.9 617,355 - +0.6 617,383 + -2.2 617,383 + +0.2 619,148 - +0.4 619,830 - CSW01_03145 0.16 +1.1 619,918 - CSW01_03145 0.22 -2.6 619,918 - CSW01_03145 0.22 +2.1 619,950 - CSW01_03145 0.24 +0.6
Or see this region's nucleotide sequence