Strain Fitness in Vibrio cholerae E7946 ATCC 55056 around CSW01_01220

Experiment: ICP1_phage_2.3_MOI_rep1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCSW01_01210 and CSW01_01215 overlap by 4 nucleotidesCSW01_01215 and CSW01_01220 overlap by 4 nucleotidesCSW01_01220 and CSW01_01225 overlap by 10 nucleotidesCSW01_01225 and CSW01_01230 overlap by 4 nucleotides CSW01_01210: CSW01_01210 - LPS biosynthesis protein WavE, at 233,673 to 234,602 _01210 CSW01_01215: CSW01_01215 - capsular biosynthesis protein, at 234,599 to 235,342 _01215 CSW01_01220: CSW01_01220 - capsular biosynthesis protein, at 235,339 to 236,922 _01220 CSW01_01225: CSW01_01225 - capsular biosynthesis protein, at 236,913 to 237,293 _01225 CSW01_01230: CSW01_01230 - 3-deoxy-D-manno-octulosonic acid transferase, at 237,290 to 238,609 _01230 Position (kb) 235 236 237Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 234.351 kb on + strand, within CSW01_01210at 234.353 kb on + strand, within CSW01_01210at 234.441 kb on - strand, within CSW01_01210at 234.446 kb on - strand, within CSW01_01210at 234.546 kb on + strandat 234.546 kb on + strandat 234.546 kb on + strandat 234.546 kb on + strandat 234.546 kb on + strandat 234.549 kb on - strandat 234.554 kb on - strandat 234.554 kb on - strandat 234.554 kb on - strandat 234.554 kb on - strandat 234.554 kb on - strandat 234.554 kb on - strandat 234.554 kb on - strandat 234.554 kb on - strandat 234.554 kb on - strandat 234.554 kb on - strandat 234.554 kb on - strandat 234.556 kb on + strandat 234.561 kb on + strandat 234.564 kb on - strandat 234.566 kb on - strandat 234.566 kb on - strandat 234.603 kb on + strandat 234.611 kb on - strandat 234.634 kb on + strandat 234.679 kb on - strand, within CSW01_01215at 234.729 kb on + strand, within CSW01_01215at 234.737 kb on - strand, within CSW01_01215at 234.737 kb on - strand, within CSW01_01215at 234.783 kb on - strand, within CSW01_01215at 234.800 kb on - strand, within CSW01_01215at 234.828 kb on + strand, within CSW01_01215at 234.828 kb on + strand, within CSW01_01215at 234.836 kb on - strand, within CSW01_01215at 234.836 kb on - strand, within CSW01_01215at 234.836 kb on - strand, within CSW01_01215at 234.900 kb on - strand, within CSW01_01215at 234.922 kb on - strand, within CSW01_01215at 234.922 kb on - strand, within CSW01_01215at 234.935 kb on - strand, within CSW01_01215at 234.937 kb on + strand, within CSW01_01215at 234.937 kb on + strand, within CSW01_01215at 234.937 kb on + strand, within CSW01_01215at 234.945 kb on - strand, within CSW01_01215at 234.945 kb on - strand, within CSW01_01215at 234.945 kb on - strand, within CSW01_01215at 234.956 kb on + strand, within CSW01_01215at 235.004 kb on + strand, within CSW01_01215at 235.010 kb on - strand, within CSW01_01215at 235.010 kb on - strand, within CSW01_01215at 235.010 kb on - strand, within CSW01_01215at 235.012 kb on - strand, within CSW01_01215at 235.021 kb on + strand, within CSW01_01215at 235.113 kb on + strand, within CSW01_01215at 235.169 kb on + strand, within CSW01_01215at 235.169 kb on + strand, within CSW01_01215at 235.177 kb on - strand, within CSW01_01215at 235.177 kb on - strand, within CSW01_01215at 235.177 kb on - strand, within CSW01_01215at 235.177 kb on - strand, within CSW01_01215at 235.186 kb on + strand, within CSW01_01215at 235.194 kb on - strand, within CSW01_01215at 235.234 kb on - strand, within CSW01_01215at 235.250 kb on + strand, within CSW01_01215at 235.256 kb on + strand, within CSW01_01215at 235.257 kb on - strand, within CSW01_01215at 235.275 kb on + strandat 235.275 kb on + strandat 235.282 kb on + strandat 235.283 kb on - strandat 235.285 kb on + strandat 235.293 kb on - strandat 235.352 kb on - strandat 235.484 kb on + strandat 235.489 kb on + strandat 235.490 kb on - strandat 235.537 kb on + strand, within CSW01_01220at 235.542 kb on + strand, within CSW01_01220at 235.578 kb on - strand, within CSW01_01220at 235.583 kb on + strand, within CSW01_01220at 235.591 kb on - strand, within CSW01_01220at 235.633 kb on + strand, within CSW01_01220at 235.697 kb on + strand, within CSW01_01220at 235.768 kb on + strand, within CSW01_01220at 235.787 kb on + strand, within CSW01_01220at 235.798 kb on + strand, within CSW01_01220at 235.866 kb on - strand, within CSW01_01220at 235.890 kb on - strand, within CSW01_01220at 235.892 kb on + strand, within CSW01_01220at 235.900 kb on - strand, within CSW01_01220at 235.946 kb on + strand, within CSW01_01220at 235.977 kb on + strand, within CSW01_01220at 235.985 kb on - strand, within CSW01_01220at 236.009 kb on - strand, within CSW01_01220at 236.011 kb on + strand, within CSW01_01220at 236.127 kb on - strand, within CSW01_01220at 236.128 kb on + strand, within CSW01_01220at 236.159 kb on + strand, within CSW01_01220at 236.248 kb on - strand, within CSW01_01220at 236.250 kb on + strand, within CSW01_01220at 236.308 kb on - strand, within CSW01_01220at 236.343 kb on + strand, within CSW01_01220at 236.343 kb on + strand, within CSW01_01220at 236.401 kb on - strand, within CSW01_01220at 236.403 kb on + strand, within CSW01_01220at 236.403 kb on + strand, within CSW01_01220at 236.403 kb on + strand, within CSW01_01220at 236.403 kb on + strand, within CSW01_01220at 236.411 kb on - strand, within CSW01_01220at 236.411 kb on - strand, within CSW01_01220at 236.419 kb on - strand, within CSW01_01220at 236.503 kb on - strand, within CSW01_01220at 236.529 kb on - strand, within CSW01_01220at 236.536 kb on + strand, within CSW01_01220at 236.545 kb on + strand, within CSW01_01220at 236.545 kb on + strand, within CSW01_01220at 236.553 kb on - strand, within CSW01_01220at 236.553 kb on - strand, within CSW01_01220at 236.553 kb on - strand, within CSW01_01220at 236.555 kb on - strand, within CSW01_01220at 236.601 kb on + strand, within CSW01_01220at 236.609 kb on + strand, within CSW01_01220at 236.609 kb on - strand, within CSW01_01220at 236.620 kb on + strand, within CSW01_01220at 236.633 kb on - strand, within CSW01_01220at 236.633 kb on - strand, within CSW01_01220at 236.633 kb on - strand, within CSW01_01220at 236.648 kb on + strand, within CSW01_01220at 236.648 kb on + strand, within CSW01_01220at 236.648 kb on + strand, within CSW01_01220at 236.654 kb on - strand, within CSW01_01220at 236.676 kb on + strand, within CSW01_01220at 236.684 kb on - strand, within CSW01_01220at 236.684 kb on - strand, within CSW01_01220at 236.732 kb on - strand, within CSW01_01220at 236.756 kb on + strand, within CSW01_01220at 236.768 kb on + strandat 236.768 kb on + strandat 236.776 kb on - strandat 236.792 kb on - strandat 236.792 kb on - strandat 236.878 kb on + strandat 236.903 kb on - strandat 236.949 kb on + strandat 237.004 kb on + strand, within CSW01_01225at 237.023 kb on - strand, within CSW01_01225at 237.025 kb on + strand, within CSW01_01225at 237.025 kb on + strand, within CSW01_01225at 237.039 kb on - strand, within CSW01_01225at 237.098 kb on + strand, within CSW01_01225at 237.128 kb on + strand, within CSW01_01225at 237.128 kb on + strand, within CSW01_01225at 237.136 kb on - strand, within CSW01_01225at 237.217 kb on - strand, within CSW01_01225at 237.227 kb on + strand, within CSW01_01225at 237.227 kb on + strand, within CSW01_01225at 237.244 kb on + strand, within CSW01_01225at 237.250 kb on + strand, within CSW01_01225

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Per-strain Table

Position Strand Gene LocusTag Fraction ICP1_phage_2.3_MOI_rep1
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234,351 + CSW01_01210 0.73 +0.4
234,353 + CSW01_01210 0.73 -3.8
234,441 - CSW01_01210 0.83 -3.0
234,446 - CSW01_01210 0.83 -4.6
234,546 + -0.5
234,546 + -0.7
234,546 + -1.6
234,546 + -2.4
234,546 + -0.6
234,549 - -1.1
234,554 - -4.1
234,554 - +0.1
234,554 - +1.0
234,554 - +1.2
234,554 - +0.6
234,554 - -2.3
234,554 - -3.1
234,554 - +1.2
234,554 - +2.9
234,554 - +0.9
234,554 - -0.3
234,556 + -3.3
234,561 + +0.0
234,564 - -0.4
234,566 - -3.7
234,566 - -4.0
234,603 + +0.3
234,611 - +0.3
234,634 + +1.3
234,679 - CSW01_01215 0.11 -4.6
234,729 + CSW01_01215 0.17 -0.4
234,737 - CSW01_01215 0.19 +0.4
234,737 - CSW01_01215 0.19 -4.8
234,783 - CSW01_01215 0.25 -2.5
234,800 - CSW01_01215 0.27 -0.4
234,828 + CSW01_01215 0.31 -1.8
234,828 + CSW01_01215 0.31 +2.4
234,836 - CSW01_01215 0.32 -0.4
234,836 - CSW01_01215 0.32 +0.2
234,836 - CSW01_01215 0.32 -2.8
234,900 - CSW01_01215 0.40 -0.7
234,922 - CSW01_01215 0.43 +0.3
234,922 - CSW01_01215 0.43 +1.0
234,935 - CSW01_01215 0.45 -0.6
234,937 + CSW01_01215 0.45 +1.1
234,937 + CSW01_01215 0.45 -0.1
234,937 + CSW01_01215 0.45 +0.3
234,945 - CSW01_01215 0.47 -2.4
234,945 - CSW01_01215 0.47 +0.9
234,945 - CSW01_01215 0.47 -0.1
234,956 + CSW01_01215 0.48 -1.6
235,004 + CSW01_01215 0.54 -0.8
235,010 - CSW01_01215 0.55 +0.7
235,010 - CSW01_01215 0.55 +1.0
235,010 - CSW01_01215 0.55 -3.3
235,012 - CSW01_01215 0.56 -0.5
235,021 + CSW01_01215 0.57 +2.0
235,113 + CSW01_01215 0.69 +2.9
235,169 + CSW01_01215 0.77 -3.5
235,169 + CSW01_01215 0.77 -2.0
235,177 - CSW01_01215 0.78 -0.2
235,177 - CSW01_01215 0.78 +1.4
235,177 - CSW01_01215 0.78 +2.8
235,177 - CSW01_01215 0.78 +1.5
235,186 + CSW01_01215 0.79 -3.2
235,194 - CSW01_01215 0.80 +1.3
235,234 - CSW01_01215 0.85 +0.6
235,250 + CSW01_01215 0.88 +1.5
235,256 + CSW01_01215 0.88 -4.0
235,257 - CSW01_01215 0.88 +0.9
235,275 + +1.0
235,275 + -2.1
235,282 + +0.7
235,283 - +0.6
235,285 + +2.0
235,293 - +1.9
235,352 - -1.0
235,484 + -1.7
235,489 + -3.2
235,490 - -0.6
235,537 + CSW01_01220 0.12 -0.8
235,542 + CSW01_01220 0.13 +0.1
235,578 - CSW01_01220 0.15 -1.5
235,583 + CSW01_01220 0.15 -0.0
235,591 - CSW01_01220 0.16 -2.8
235,633 + CSW01_01220 0.19 -2.6
235,697 + CSW01_01220 0.23 -0.2
235,768 + CSW01_01220 0.27 -0.3
235,787 + CSW01_01220 0.28 +1.1
235,798 + CSW01_01220 0.29 -0.6
235,866 - CSW01_01220 0.33 -1.4
235,890 - CSW01_01220 0.35 -0.3
235,892 + CSW01_01220 0.35 -3.9
235,900 - CSW01_01220 0.35 -0.7
235,946 + CSW01_01220 0.38 +0.1
235,977 + CSW01_01220 0.40 +3.0
235,985 - CSW01_01220 0.41 +0.1
236,009 - CSW01_01220 0.42 -1.3
236,011 + CSW01_01220 0.42 -0.1
236,127 - CSW01_01220 0.50 -0.7
236,128 + CSW01_01220 0.50 +0.1
236,159 + CSW01_01220 0.52 -2.9
236,248 - CSW01_01220 0.57 +0.3
236,250 + CSW01_01220 0.58 +1.4
236,308 - CSW01_01220 0.61 -1.3
236,343 + CSW01_01220 0.63 -2.0
236,343 + CSW01_01220 0.63 -0.9
236,401 - CSW01_01220 0.67 +0.6
236,403 + CSW01_01220 0.67 -2.8
236,403 + CSW01_01220 0.67 -0.8
236,403 + CSW01_01220 0.67 +0.8
236,403 + CSW01_01220 0.67 +0.7
236,411 - CSW01_01220 0.68 -0.0
236,411 - CSW01_01220 0.68 -3.2
236,419 - CSW01_01220 0.68 +0.3
236,503 - CSW01_01220 0.73 +0.8
236,529 - CSW01_01220 0.75 -0.7
236,536 + CSW01_01220 0.76 -3.0
236,545 + CSW01_01220 0.76 -1.5
236,545 + CSW01_01220 0.76 +0.9
236,553 - CSW01_01220 0.77 -0.3
236,553 - CSW01_01220 0.77 -0.5
236,553 - CSW01_01220 0.77 +1.1
236,555 - CSW01_01220 0.77 -1.5
236,601 + CSW01_01220 0.80 -0.8
236,609 + CSW01_01220 0.80 -2.1
236,609 - CSW01_01220 0.80 -0.3
236,620 + CSW01_01220 0.81 +0.1
236,633 - CSW01_01220 0.82 +0.1
236,633 - CSW01_01220 0.82 -0.3
236,633 - CSW01_01220 0.82 -0.9
236,648 + CSW01_01220 0.83 -2.3
236,648 + CSW01_01220 0.83 +0.2
236,648 + CSW01_01220 0.83 +0.2
236,654 - CSW01_01220 0.83 -0.7
236,676 + CSW01_01220 0.84 +1.7
236,684 - CSW01_01220 0.85 -2.3
236,684 - CSW01_01220 0.85 -2.3
236,732 - CSW01_01220 0.88 -3.2
236,756 + CSW01_01220 0.89 +1.6
236,768 + -2.1
236,768 + -0.2
236,776 - -1.0
236,792 - +0.8
236,792 - -4.0
236,878 + -0.4
236,903 - -3.2
236,949 + -0.6
237,004 + CSW01_01225 0.24 -0.2
237,023 - CSW01_01225 0.29 -1.7
237,025 + CSW01_01225 0.29 +0.6
237,025 + CSW01_01225 0.29 -4.4
237,039 - CSW01_01225 0.33 +1.3
237,098 + CSW01_01225 0.49 +0.8
237,128 + CSW01_01225 0.56 -2.4
237,128 + CSW01_01225 0.56 -0.5
237,136 - CSW01_01225 0.59 +0.9
237,217 - CSW01_01225 0.80 +1.3
237,227 + CSW01_01225 0.82 +0.5
237,227 + CSW01_01225 0.82 +0.3
237,244 + CSW01_01225 0.87 -0.5
237,250 + CSW01_01225 0.88 +1.0

Or see this region's nucleotide sequence