Experiment: Anaero. fumarate as e acceptor
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1420 and Shewana3_1421 are separated by 128 nucleotides Shewana3_1421 and Shewana3_1422 are separated by 37 nucleotides
Shewana3_1420: Shewana3_1420 - transcriptional regulator Ada / DNA-O6-methylguanine--protein-cysteine S-methyltransferase / DNA-3-methyladenine glycosylase II (RefSeq), at 1,649,381 to 1,651,009
_1420
Shewana3_1421: Shewana3_1421 - methylated-DNA--protein-cysteine methyltransferase (RefSeq), at 1,651,138 to 1,651,704
_1421
Shewana3_1422: Shewana3_1422 - 4a-hydroxytetrahydrobiopterin dehydratase (RefSeq), at 1,651,742 to 1,653,025
_1422
Position (kb)
1651
1652 Strain fitness (log2 ratio)
-2
-1
0
1 at 1650.284 kb on + strand, within Shewana3_1420 at 1650.284 kb on + strand, within Shewana3_1420 at 1650.292 kb on - strand, within Shewana3_1420 at 1650.342 kb on + strand, within Shewana3_1420 at 1650.442 kb on + strand, within Shewana3_1420 at 1650.641 kb on + strand, within Shewana3_1420 at 1651.132 kb on + strand at 1651.146 kb on + strand at 1651.335 kb on + strand, within Shewana3_1421 at 1651.335 kb on + strand, within Shewana3_1421 at 1651.412 kb on + strand, within Shewana3_1421 at 1651.529 kb on - strand, within Shewana3_1421 at 1651.825 kb on - strand at 1652.577 kb on + strand, within Shewana3_1422
Per-strain Table
Position Strand Gene LocusTag Fraction Anaero. fumarate as e acceptor remove 1,650,284 + Shewana3_1420 0.55 +0.2 1,650,284 + Shewana3_1420 0.55 -1.9 1,650,292 - Shewana3_1420 0.56 -0.6 1,650,342 + Shewana3_1420 0.59 -1.9 1,650,442 + Shewana3_1420 0.65 -0.2 1,650,641 + Shewana3_1420 0.77 -0.1 1,651,132 + -1.1 1,651,146 + +0.4 1,651,335 + Shewana3_1421 0.35 -1.1 1,651,335 + Shewana3_1421 0.35 -0.8 1,651,412 + Shewana3_1421 0.48 -1.6 1,651,529 - Shewana3_1421 0.69 +0.4 1,651,825 - +0.4 1,652,577 + Shewana3_1422 0.65 -0.6
Or see this region's nucleotide sequence