Experiment: Anaero. fumarate as e acceptor
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1010 and Shewana3_1011 are separated by 167 nucleotides Shewana3_1011 and Shewana3_1012 are separated by 139 nucleotides Shewana3_1012 and Shewana3_R0031 are separated by 207 nucleotides Shewana3_R0031 and Shewana3_R0032 are separated by 54 nucleotides Shewana3_R0032 and Shewana3_R0033 are separated by 47 nucleotides Shewana3_R0033 and Shewana3_R0034 are separated by 22 nucleotides Shewana3_R0034 and Shewana3_R0035 are separated by 1 nucleotides Shewana3_R0035 and Shewana3_R0036 are separated by 83 nucleotides Shewana3_R0036 and Shewana3_R0037 are separated by 36 nucleotides
Shewana3_1010: Shewana3_1010 - 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (RefSeq), at 1,180,756 to 1,181,982
_1010
Shewana3_1011: Shewana3_1011 - peptidyl-tRNA hydrolase (RefSeq), at 1,182,150 to 1,182,737
_1011
Shewana3_1012: Shewana3_1012 - GTP-dependent nucleic acid-binding protein EngD (RefSeq), at 1,182,877 to 1,183,968
_1012
Shewana3_R0031: Shewana3_R0031 - tRNA-Met (RefSeq), at 1,184,176 to 1,184,252
_R0031
Shewana3_R0032: Shewana3_R0032 - tRNA-Leu (RefSeq), at 1,184,307 to 1,184,391
_R0032
Shewana3_R0033: Shewana3_R0033 - tRNA-Gln (RefSeq), at 1,184,439 to 1,184,513
_R0033
Shewana3_R0034: Shewana3_R0034 - tRNA-Gln (RefSeq), at 1,184,536 to 1,184,610
_R0034
Shewana3_R0035: Shewana3_R0035 - tRNA-Met (RefSeq), at 1,184,612 to 1,184,688
_R0035
Shewana3_R0036: Shewana3_R0036 - tRNA-Leu (RefSeq), at 1,184,772 to 1,184,856
_R0036
Shewana3_R0037: Shewana3_R0037 - tRNA-Gln (RefSeq), at 1,184,893 to 1,184,967
_R0037
Position (kb)
1182
1183
1184 Strain fitness (log2 ratio)
-2
-1
0
1 at 1182.003 kb on - strand at 1182.689 kb on + strand at 1182.711 kb on + strand at 1182.722 kb on + strand at 1182.740 kb on - strand at 1182.802 kb on + strand at 1182.802 kb on + strand at 1182.810 kb on - strand at 1182.881 kb on - strand at 1183.108 kb on - strand, within Shewana3_1012 at 1183.230 kb on + strand, within Shewana3_1012 at 1183.292 kb on + strand, within Shewana3_1012 at 1183.292 kb on + strand, within Shewana3_1012 at 1183.300 kb on - strand, within Shewana3_1012 at 1183.469 kb on + strand, within Shewana3_1012 at 1183.655 kb on + strand, within Shewana3_1012 at 1183.693 kb on + strand, within Shewana3_1012 at 1183.757 kb on - strand, within Shewana3_1012 at 1183.819 kb on + strand, within Shewana3_1012 at 1183.916 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Anaero. fumarate as e acceptor remove 1,182,003 - +0.4 1,182,689 + -0.6 1,182,711 + -2.7 1,182,722 + -0.6 1,182,740 - -1.6 1,182,802 + -0.2 1,182,802 + -0.2 1,182,810 - -1.4 1,182,881 - +1.0 1,183,108 - Shewana3_1012 0.21 -1.2 1,183,230 + Shewana3_1012 0.32 -0.5 1,183,292 + Shewana3_1012 0.38 -1.5 1,183,292 + Shewana3_1012 0.38 -1.1 1,183,300 - Shewana3_1012 0.39 -1.3 1,183,469 + Shewana3_1012 0.54 -1.7 1,183,655 + Shewana3_1012 0.71 -1.1 1,183,693 + Shewana3_1012 0.75 -1.0 1,183,757 - Shewana3_1012 0.81 -0.3 1,183,819 + Shewana3_1012 0.86 -0.8 1,183,916 + -0.2
Or see this region's nucleotide sequence