Experiment: MoYLS4 with HQNO 100uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pilT-1 and DORF10286 are separated by 67 nucleotides DORF10286 and DVU1271 are separated by 112 nucleotides DVU1271 and gspE are separated by 3 nucleotides
DVU1270: pilT-1 - twitching motility protein (TIGR), at 1,359,233 to 1,360,513
pilT-1
DORF10286: DORF10286 - no description, at 1,360,581 to 1,361,006
DORF10286
DVU1271: DVU1271 - general secretion pathway protein F, putative (TIGR), at 1,361,119 to 1,362,345
DVU1271
DVU1272: gspE - general secretion pathway protein E, putative (TIGR), at 1,362,349 to 1,364,070
gspE
Position (kb)
1361
1362
1363 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1360.203 kb on - strand, within pilT-1 at 1360.231 kb on + strand, within pilT-1 at 1360.267 kb on - strand, within pilT-1 at 1360.346 kb on - strand, within pilT-1 at 1360.456 kb on + strand at 1360.573 kb on + strand at 1360.599 kb on + strand at 1360.618 kb on - strand at 1360.627 kb on - strand, within DORF10286 at 1360.691 kb on - strand, within DORF10286 at 1360.732 kb on - strand, within DORF10286 at 1360.876 kb on + strand, within DORF10286 at 1360.879 kb on - strand, within DORF10286 at 1360.935 kb on + strand, within DORF10286 at 1360.961 kb on + strand, within DORF10286 at 1360.970 kb on - strand at 1361.043 kb on + strand at 1361.076 kb on - strand at 1361.110 kb on + strand at 1361.125 kb on - strand at 1361.248 kb on + strand, within DVU1271 at 1361.295 kb on + strand, within DVU1271 at 1361.332 kb on - strand, within DVU1271 at 1361.373 kb on - strand, within DVU1271 at 1361.436 kb on - strand, within DVU1271 at 1361.450 kb on + strand, within DVU1271 at 1361.489 kb on - strand, within DVU1271 at 1361.520 kb on - strand, within DVU1271 at 1361.587 kb on - strand, within DVU1271 at 1361.661 kb on + strand, within DVU1271 at 1361.822 kb on + strand, within DVU1271 at 1361.825 kb on - strand, within DVU1271 at 1361.830 kb on - strand, within DVU1271 at 1361.890 kb on + strand, within DVU1271 at 1361.898 kb on - strand, within DVU1271 at 1361.905 kb on - strand, within DVU1271 at 1361.911 kb on + strand, within DVU1271 at 1361.959 kb on - strand, within DVU1271 at 1361.991 kb on + strand, within DVU1271 at 1361.994 kb on - strand, within DVU1271 at 1362.089 kb on - strand, within DVU1271 at 1362.113 kb on + strand, within DVU1271 at 1362.174 kb on + strand, within DVU1271 at 1362.228 kb on + strand at 1362.274 kb on - strand at 1362.410 kb on - strand at 1362.439 kb on + strand at 1362.439 kb on + strand at 1362.447 kb on - strand at 1362.495 kb on + strand at 1362.538 kb on - strand, within gspE at 1362.548 kb on - strand, within gspE at 1362.598 kb on - strand, within gspE at 1362.599 kb on - strand, within gspE at 1362.613 kb on + strand, within gspE at 1362.807 kb on + strand, within gspE at 1362.861 kb on + strand, within gspE at 1362.896 kb on - strand, within gspE at 1362.940 kb on + strand, within gspE at 1362.948 kb on - strand, within gspE at 1363.005 kb on + strand, within gspE at 1363.045 kb on + strand, within gspE at 1363.089 kb on + strand, within gspE at 1363.186 kb on - strand, within gspE at 1363.186 kb on - strand, within gspE at 1363.215 kb on - strand, within gspE
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with HQNO 100uM remove 1,360,203 - pilT-1 DVU1270 0.76 +0.3 1,360,231 + pilT-1 DVU1270 0.78 -0.1 1,360,267 - pilT-1 DVU1270 0.81 +0.5 1,360,346 - pilT-1 DVU1270 0.87 -0.0 1,360,456 + -1.0 1,360,573 + +0.9 1,360,599 + +0.3 1,360,618 - +0.9 1,360,627 - DORF10286 0.11 +0.6 1,360,691 - DORF10286 0.26 +0.8 1,360,732 - DORF10286 0.35 +0.7 1,360,876 + DORF10286 0.69 +0.5 1,360,879 - DORF10286 0.70 -0.4 1,360,935 + DORF10286 0.83 -0.2 1,360,961 + DORF10286 0.89 +0.1 1,360,970 - +0.4 1,361,043 + +0.9 1,361,076 - -0.2 1,361,110 + -0.1 1,361,125 - -0.7 1,361,248 + DVU1271 0.11 +0.6 1,361,295 + DVU1271 0.14 +0.3 1,361,332 - DVU1271 0.17 +0.9 1,361,373 - DVU1271 0.21 +0.6 1,361,436 - DVU1271 0.26 +0.9 1,361,450 + DVU1271 0.27 +2.1 1,361,489 - DVU1271 0.30 +0.7 1,361,520 - DVU1271 0.33 +0.8 1,361,587 - DVU1271 0.38 -0.1 1,361,661 + DVU1271 0.44 -2.0 1,361,822 + DVU1271 0.57 +0.5 1,361,825 - DVU1271 0.58 +0.5 1,361,830 - DVU1271 0.58 +0.6 1,361,890 + DVU1271 0.63 +0.5 1,361,898 - DVU1271 0.63 +1.5 1,361,905 - DVU1271 0.64 +0.5 1,361,911 + DVU1271 0.65 -0.3 1,361,959 - DVU1271 0.68 +0.7 1,361,991 + DVU1271 0.71 -0.6 1,361,994 - DVU1271 0.71 +0.6 1,362,089 - DVU1271 0.79 +1.3 1,362,113 + DVU1271 0.81 -0.2 1,362,174 + DVU1271 0.86 +0.4 1,362,228 + +0.4 1,362,274 - +0.5 1,362,410 - +0.6 1,362,439 + +0.2 1,362,439 + -0.3 1,362,447 - +0.3 1,362,495 + +0.6 1,362,538 - gspE DVU1272 0.11 +0.7 1,362,548 - gspE DVU1272 0.12 +0.6 1,362,598 - gspE DVU1272 0.14 +0.5 1,362,599 - gspE DVU1272 0.15 -0.7 1,362,613 + gspE DVU1272 0.15 +0.6 1,362,807 + gspE DVU1272 0.27 +0.6 1,362,861 + gspE DVU1272 0.30 +0.6 1,362,896 - gspE DVU1272 0.32 +0.4 1,362,940 + gspE DVU1272 0.34 +1.3 1,362,948 - gspE DVU1272 0.35 +0.8 1,363,005 + gspE DVU1272 0.38 -0.1 1,363,045 + gspE DVU1272 0.40 +0.7 1,363,089 + gspE DVU1272 0.43 +0.2 1,363,186 - gspE DVU1272 0.49 +0.5 1,363,186 - gspE DVU1272 0.49 +0.4 1,363,215 - gspE DVU1272 0.50 +0.4
Or see this region's nucleotide sequence