Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_80 and Pf1N1B4_81 are separated by 168 nucleotides Pf1N1B4_81 and Pf1N1B4_82 are separated by 441 nucleotides
Pf1N1B4_80 - Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77), at 82,561 to 83,844
_80
Pf1N1B4_81 - Putative sulfate permease, at 84,013 to 84,798
_81
Pf1N1B4_82 - Mobile element protein, at 85,240 to 86,181
_82
Position (kb)
84
85 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 83.040 kb on + strand, within Pf1N1B4_80 at 83.041 kb on - strand, within Pf1N1B4_80 at 83.165 kb on + strand, within Pf1N1B4_80 at 83.166 kb on - strand, within Pf1N1B4_80 at 83.364 kb on + strand, within Pf1N1B4_80 at 83.364 kb on + strand at 83.365 kb on - strand, within Pf1N1B4_80 at 83.365 kb on - strand, within Pf1N1B4_80 at 83.366 kb on + strand, within Pf1N1B4_80 at 83.423 kb on - strand, within Pf1N1B4_80 at 83.423 kb on - strand, within Pf1N1B4_80 at 83.450 kb on + strand, within Pf1N1B4_80 at 83.544 kb on + strand, within Pf1N1B4_80 at 83.545 kb on - strand, within Pf1N1B4_80 at 83.717 kb on + strand at 83.717 kb on + strand at 83.718 kb on - strand at 83.840 kb on - strand at 83.894 kb on - strand at 83.905 kb on + strand at 83.950 kb on + strand at 83.999 kb on + strand at 83.999 kb on + strand at 83.999 kb on + strand at 83.999 kb on + strand at 84.000 kb on - strand at 84.000 kb on - strand at 84.000 kb on - strand at 84.000 kb on - strand at 84.000 kb on - strand at 84.000 kb on - strand at 84.000 kb on - strand at 84.000 kb on - strand at 84.000 kb on - strand at 84.000 kb on - strand at 84.000 kb on - strand at 84.000 kb on - strand at 84.000 kb on - strand at 84.000 kb on - strand at 84.000 kb on - strand at 84.000 kb on - strand at 84.001 kb on + strand at 84.001 kb on + strand at 84.030 kb on - strand at 84.110 kb on + strand, within Pf1N1B4_81 at 84.140 kb on + strand, within Pf1N1B4_81 at 84.163 kb on - strand, within Pf1N1B4_81 at 84.383 kb on + strand, within Pf1N1B4_81 at 84.460 kb on + strand, within Pf1N1B4_81 at 84.647 kb on + strand, within Pf1N1B4_81 at 84.855 kb on + strand at 84.858 kb on - strand at 84.866 kb on - strand at 84.881 kb on - strand at 84.883 kb on - strand at 84.981 kb on + strand at 84.981 kb on + strand at 85.035 kb on - strand at 85.035 kb on - strand at 85.035 kb on - strand at 85.035 kb on - strand at 85.035 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 83,040 + Pf1N1B4_80 0.37 -0.8 83,041 - Pf1N1B4_80 0.37 -0.2 83,165 + Pf1N1B4_80 0.47 -1.9 83,166 - Pf1N1B4_80 0.47 +2.9 83,364 + Pf1N1B4_80 0.63 +1.3 83,364 + -0.2 83,365 - Pf1N1B4_80 0.63 -1.1 83,365 - Pf1N1B4_80 0.63 -0.2 83,366 + Pf1N1B4_80 0.63 +0.8 83,423 - Pf1N1B4_80 0.67 -0.4 83,423 - Pf1N1B4_80 0.67 +0.0 83,450 + Pf1N1B4_80 0.69 +1.3 83,544 + Pf1N1B4_80 0.77 +0.6 83,545 - Pf1N1B4_80 0.77 +1.1 83,717 + +0.8 83,717 + -1.8 83,718 - +0.2 83,840 - -1.0 83,894 - -1.8 83,905 + +0.3 83,950 + -0.3 83,999 + -2.8 83,999 + -0.5 83,999 + -0.2 83,999 + -0.2 84,000 - -0.8 84,000 - -1.2 84,000 - +1.3 84,000 - -0.2 84,000 - -1.8 84,000 - +0.3 84,000 - +0.2 84,000 - -0.2 84,000 - -0.6 84,000 - +0.8 84,000 - -0.6 84,000 - +0.2 84,000 - -0.8 84,000 - +0.1 84,000 - -1.4 84,000 - -2.2 84,001 + -0.2 84,001 + +0.5 84,030 - +0.8 84,110 + Pf1N1B4_81 0.12 -0.7 84,140 + Pf1N1B4_81 0.16 -1.2 84,163 - Pf1N1B4_81 0.19 -1.6 84,383 + Pf1N1B4_81 0.47 -1.4 84,460 + Pf1N1B4_81 0.57 -0.4 84,647 + Pf1N1B4_81 0.81 -0.2 84,855 + -1.0 84,858 - -2.8 84,866 - +0.3 84,881 - +2.6 84,883 - -1.6 84,981 + -2.2 84,981 + +0.8 85,035 - +0.8 85,035 - +0.8 85,035 - -1.1 85,035 - -2.6 85,035 - -0.2
Or see this region's nucleotide sequence