Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5705

Experiment: L-Leucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5704 and Pf1N1B4_5705 are separated by 162 nucleotidesPf1N1B4_5705 and Pf1N1B4_5706 overlap by 1 nucleotides Pf1N1B4_5704 - Long-chain fatty acid transport protein, at 141,156 to 142,475 _5704 Pf1N1B4_5705 - Acetyl-coenzyme A synthetase (EC 6.2.1.1), at 142,638 to 144,539 _5705 Pf1N1B4_5706 - Fumarylacetoacetate hydrolase family protein, at 144,539 to 145,660 _5706 Position (kb) 142 143 144 145Strain fitness (log2 ratio) -2 -1 0 1 2at 141.660 kb on - strand, within Pf1N1B4_5704at 141.660 kb on - strand, within Pf1N1B4_5704at 141.666 kb on + strand, within Pf1N1B4_5704at 141.667 kb on - strand, within Pf1N1B4_5704at 141.667 kb on - strand, within Pf1N1B4_5704at 141.679 kb on + strand, within Pf1N1B4_5704at 141.680 kb on - strand, within Pf1N1B4_5704at 141.680 kb on - strand, within Pf1N1B4_5704at 141.743 kb on - strand, within Pf1N1B4_5704at 141.761 kb on - strand, within Pf1N1B4_5704at 141.761 kb on - strand, within Pf1N1B4_5704at 141.890 kb on - strand, within Pf1N1B4_5704at 141.946 kb on + strand, within Pf1N1B4_5704at 142.004 kb on - strand, within Pf1N1B4_5704at 142.039 kb on + strand, within Pf1N1B4_5704at 142.040 kb on - strand, within Pf1N1B4_5704at 142.040 kb on - strand, within Pf1N1B4_5704at 142.066 kb on + strand, within Pf1N1B4_5704at 142.112 kb on + strand, within Pf1N1B4_5704at 142.113 kb on - strandat 142.113 kb on - strandat 142.113 kb on - strandat 142.113 kb on - strand, within Pf1N1B4_5704at 142.114 kb on + strand, within Pf1N1B4_5704at 142.173 kb on - strand, within Pf1N1B4_5704at 142.270 kb on + strand, within Pf1N1B4_5704at 142.270 kb on + strand, within Pf1N1B4_5704at 142.270 kb on + strand, within Pf1N1B4_5704at 142.426 kb on + strandat 142.427 kb on - strandat 142.427 kb on - strandat 142.491 kb on + strandat 142.491 kb on + strandat 142.619 kb on - strandat 142.619 kb on - strandat 142.619 kb on - strandat 142.644 kb on - strandat 142.665 kb on - strandat 142.665 kb on - strandat 142.665 kb on - strandat 142.801 kb on - strandat 142.839 kb on + strand, within Pf1N1B4_5705at 142.840 kb on - strand, within Pf1N1B4_5705at 142.978 kb on + strand, within Pf1N1B4_5705at 143.003 kb on - strand, within Pf1N1B4_5705at 143.217 kb on - strand, within Pf1N1B4_5705at 143.303 kb on - strand, within Pf1N1B4_5705at 143.336 kb on + strand, within Pf1N1B4_5705at 143.875 kb on + strand, within Pf1N1B4_5705at 143.876 kb on - strand, within Pf1N1B4_5705at 143.979 kb on + strand, within Pf1N1B4_5705at 144.037 kb on + strandat 144.038 kb on - strand, within Pf1N1B4_5705at 144.038 kb on - strand, within Pf1N1B4_5705at 144.180 kb on + strandat 144.181 kb on - strand, within Pf1N1B4_5705at 144.278 kb on + strand, within Pf1N1B4_5705at 144.279 kb on - strand, within Pf1N1B4_5705at 144.279 kb on - strand, within Pf1N1B4_5705at 144.303 kb on + strand, within Pf1N1B4_5705at 144.304 kb on - strand, within Pf1N1B4_5705at 144.304 kb on - strand, within Pf1N1B4_5705at 144.362 kb on - strandat 144.362 kb on - strandat 144.373 kb on + strandat 144.373 kb on + strandat 144.373 kb on + strandat 144.460 kb on + strandat 144.461 kb on - strandat 144.465 kb on - strandat 144.637 kb on - strandat 144.884 kb on + strand, within Pf1N1B4_5706at 144.885 kb on - strand, within Pf1N1B4_5706at 144.910 kb on + strand, within Pf1N1B4_5706at 144.978 kb on + strand, within Pf1N1B4_5706at 145.436 kb on + strand, within Pf1N1B4_5706at 145.479 kb on - strand, within Pf1N1B4_5706at 145.479 kb on - strand, within Pf1N1B4_5706at 145.484 kb on + strand, within Pf1N1B4_5706at 145.502 kb on + strand, within Pf1N1B4_5706

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Leucine (C)
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141,660 - Pf1N1B4_5704 0.38 -1.3
141,660 - Pf1N1B4_5704 0.38 +2.0
141,666 + Pf1N1B4_5704 0.39 +0.7
141,667 - Pf1N1B4_5704 0.39 +2.0
141,667 - Pf1N1B4_5704 0.39 -0.3
141,679 + Pf1N1B4_5704 0.40 -1.2
141,680 - Pf1N1B4_5704 0.40 -1.3
141,680 - Pf1N1B4_5704 0.40 -0.3
141,743 - Pf1N1B4_5704 0.44 +0.7
141,761 - Pf1N1B4_5704 0.46 -0.9
141,761 - Pf1N1B4_5704 0.46 -1.1
141,890 - Pf1N1B4_5704 0.56 +0.5
141,946 + Pf1N1B4_5704 0.60 -1.0
142,004 - Pf1N1B4_5704 0.64 +1.8
142,039 + Pf1N1B4_5704 0.67 -0.3
142,040 - Pf1N1B4_5704 0.67 +1.8
142,040 - Pf1N1B4_5704 0.67 +0.7
142,066 + Pf1N1B4_5704 0.69 -0.7
142,112 + Pf1N1B4_5704 0.72 -2.3
142,113 - +0.7
142,113 - -0.3
142,113 - +2.0
142,113 - Pf1N1B4_5704 0.72 +0.1
142,114 + Pf1N1B4_5704 0.73 -1.0
142,173 - Pf1N1B4_5704 0.77 +0.3
142,270 + Pf1N1B4_5704 0.84 +1.3
142,270 + Pf1N1B4_5704 0.84 -1.7
142,270 + Pf1N1B4_5704 0.84 -0.5
142,426 + -2.3
142,427 - -0.3
142,427 - +0.6
142,491 + +2.3
142,491 + +0.7
142,619 - +0.1
142,619 - -0.1
142,619 - +1.3
142,644 - +0.3
142,665 - -0.9
142,665 - -0.5
142,665 - +1.3
142,801 - -0.9
142,839 + Pf1N1B4_5705 0.11 -0.3
142,840 - Pf1N1B4_5705 0.11 -0.3
142,978 + Pf1N1B4_5705 0.18 -0.9
143,003 - Pf1N1B4_5705 0.19 -0.3
143,217 - Pf1N1B4_5705 0.30 +2.3
143,303 - Pf1N1B4_5705 0.35 -0.0
143,336 + Pf1N1B4_5705 0.37 -0.3
143,875 + Pf1N1B4_5705 0.65 -1.7
143,876 - Pf1N1B4_5705 0.65 +2.3
143,979 + Pf1N1B4_5705 0.71 -1.6
144,037 + -1.3
144,038 - Pf1N1B4_5705 0.74 +1.3
144,038 - Pf1N1B4_5705 0.74 -0.7
144,180 + -1.9
144,181 - Pf1N1B4_5705 0.81 +2.0
144,278 + Pf1N1B4_5705 0.86 -0.0
144,279 - Pf1N1B4_5705 0.86 +0.5
144,279 - Pf1N1B4_5705 0.86 -0.0
144,303 + Pf1N1B4_5705 0.88 -0.3
144,304 - Pf1N1B4_5705 0.88 -1.9
144,304 - Pf1N1B4_5705 0.88 -0.3
144,362 - -1.3
144,362 - -1.1
144,373 + +0.3
144,373 + -0.3
144,373 + -0.1
144,460 + -0.3
144,461 - +0.1
144,465 - -0.0
144,637 - +0.3
144,884 + Pf1N1B4_5706 0.31 +0.4
144,885 - Pf1N1B4_5706 0.31 -0.3
144,910 + Pf1N1B4_5706 0.33 -2.2
144,978 + Pf1N1B4_5706 0.39 -0.5
145,436 + Pf1N1B4_5706 0.80 +0.1
145,479 - Pf1N1B4_5706 0.84 -1.3
145,479 - Pf1N1B4_5706 0.84 +0.3
145,484 + Pf1N1B4_5706 0.84 +1.7
145,502 + Pf1N1B4_5706 0.86 +0.2

Or see this region's nucleotide sequence