Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5704 and Pf1N1B4_5705 are separated by 162 nucleotides Pf1N1B4_5705 and Pf1N1B4_5706 overlap by 1 nucleotides
Pf1N1B4_5704 - Long-chain fatty acid transport protein, at 141,156 to 142,475
_5704
Pf1N1B4_5705 - Acetyl-coenzyme A synthetase (EC 6.2.1.1), at 142,638 to 144,539
_5705
Pf1N1B4_5706 - Fumarylacetoacetate hydrolase family protein, at 144,539 to 145,660
_5706
Position (kb)
142
143
144
145 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 141.660 kb on - strand, within Pf1N1B4_5704 at 141.660 kb on - strand, within Pf1N1B4_5704 at 141.666 kb on + strand, within Pf1N1B4_5704 at 141.667 kb on - strand, within Pf1N1B4_5704 at 141.667 kb on - strand, within Pf1N1B4_5704 at 141.679 kb on + strand, within Pf1N1B4_5704 at 141.680 kb on - strand, within Pf1N1B4_5704 at 141.680 kb on - strand, within Pf1N1B4_5704 at 141.743 kb on - strand, within Pf1N1B4_5704 at 141.761 kb on - strand, within Pf1N1B4_5704 at 141.761 kb on - strand, within Pf1N1B4_5704 at 141.890 kb on - strand, within Pf1N1B4_5704 at 141.946 kb on + strand, within Pf1N1B4_5704 at 142.004 kb on - strand, within Pf1N1B4_5704 at 142.039 kb on + strand, within Pf1N1B4_5704 at 142.040 kb on - strand, within Pf1N1B4_5704 at 142.040 kb on - strand, within Pf1N1B4_5704 at 142.066 kb on + strand, within Pf1N1B4_5704 at 142.112 kb on + strand, within Pf1N1B4_5704 at 142.113 kb on - strand at 142.113 kb on - strand at 142.113 kb on - strand at 142.113 kb on - strand, within Pf1N1B4_5704 at 142.114 kb on + strand, within Pf1N1B4_5704 at 142.173 kb on - strand, within Pf1N1B4_5704 at 142.270 kb on + strand, within Pf1N1B4_5704 at 142.270 kb on + strand, within Pf1N1B4_5704 at 142.270 kb on + strand, within Pf1N1B4_5704 at 142.426 kb on + strand at 142.427 kb on - strand at 142.427 kb on - strand at 142.491 kb on + strand at 142.491 kb on + strand at 142.619 kb on - strand at 142.619 kb on - strand at 142.619 kb on - strand at 142.644 kb on - strand at 142.665 kb on - strand at 142.665 kb on - strand at 142.665 kb on - strand at 142.801 kb on - strand at 142.839 kb on + strand, within Pf1N1B4_5705 at 142.840 kb on - strand, within Pf1N1B4_5705 at 142.978 kb on + strand, within Pf1N1B4_5705 at 143.003 kb on - strand, within Pf1N1B4_5705 at 143.217 kb on - strand, within Pf1N1B4_5705 at 143.303 kb on - strand, within Pf1N1B4_5705 at 143.336 kb on + strand, within Pf1N1B4_5705 at 143.875 kb on + strand, within Pf1N1B4_5705 at 143.876 kb on - strand, within Pf1N1B4_5705 at 143.979 kb on + strand, within Pf1N1B4_5705 at 144.037 kb on + strand at 144.038 kb on - strand, within Pf1N1B4_5705 at 144.038 kb on - strand, within Pf1N1B4_5705 at 144.180 kb on + strand at 144.181 kb on - strand, within Pf1N1B4_5705 at 144.278 kb on + strand, within Pf1N1B4_5705 at 144.279 kb on - strand, within Pf1N1B4_5705 at 144.279 kb on - strand, within Pf1N1B4_5705 at 144.303 kb on + strand, within Pf1N1B4_5705 at 144.304 kb on - strand, within Pf1N1B4_5705 at 144.304 kb on - strand, within Pf1N1B4_5705 at 144.362 kb on - strand at 144.362 kb on - strand at 144.373 kb on + strand at 144.373 kb on + strand at 144.373 kb on + strand at 144.460 kb on + strand at 144.461 kb on - strand at 144.465 kb on - strand at 144.637 kb on - strand at 144.884 kb on + strand, within Pf1N1B4_5706 at 144.885 kb on - strand, within Pf1N1B4_5706 at 144.910 kb on + strand, within Pf1N1B4_5706 at 144.978 kb on + strand, within Pf1N1B4_5706 at 145.436 kb on + strand, within Pf1N1B4_5706 at 145.479 kb on - strand, within Pf1N1B4_5706 at 145.479 kb on - strand, within Pf1N1B4_5706 at 145.484 kb on + strand, within Pf1N1B4_5706 at 145.502 kb on + strand, within Pf1N1B4_5706
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 141,660 - Pf1N1B4_5704 0.38 -1.3 141,660 - Pf1N1B4_5704 0.38 +2.0 141,666 + Pf1N1B4_5704 0.39 +0.7 141,667 - Pf1N1B4_5704 0.39 +2.0 141,667 - Pf1N1B4_5704 0.39 -0.3 141,679 + Pf1N1B4_5704 0.40 -1.2 141,680 - Pf1N1B4_5704 0.40 -1.3 141,680 - Pf1N1B4_5704 0.40 -0.3 141,743 - Pf1N1B4_5704 0.44 +0.7 141,761 - Pf1N1B4_5704 0.46 -0.9 141,761 - Pf1N1B4_5704 0.46 -1.1 141,890 - Pf1N1B4_5704 0.56 +0.5 141,946 + Pf1N1B4_5704 0.60 -1.0 142,004 - Pf1N1B4_5704 0.64 +1.8 142,039 + Pf1N1B4_5704 0.67 -0.3 142,040 - Pf1N1B4_5704 0.67 +1.8 142,040 - Pf1N1B4_5704 0.67 +0.7 142,066 + Pf1N1B4_5704 0.69 -0.7 142,112 + Pf1N1B4_5704 0.72 -2.3 142,113 - +0.7 142,113 - -0.3 142,113 - +2.0 142,113 - Pf1N1B4_5704 0.72 +0.1 142,114 + Pf1N1B4_5704 0.73 -1.0 142,173 - Pf1N1B4_5704 0.77 +0.3 142,270 + Pf1N1B4_5704 0.84 +1.3 142,270 + Pf1N1B4_5704 0.84 -1.7 142,270 + Pf1N1B4_5704 0.84 -0.5 142,426 + -2.3 142,427 - -0.3 142,427 - +0.6 142,491 + +2.3 142,491 + +0.7 142,619 - +0.1 142,619 - -0.1 142,619 - +1.3 142,644 - +0.3 142,665 - -0.9 142,665 - -0.5 142,665 - +1.3 142,801 - -0.9 142,839 + Pf1N1B4_5705 0.11 -0.3 142,840 - Pf1N1B4_5705 0.11 -0.3 142,978 + Pf1N1B4_5705 0.18 -0.9 143,003 - Pf1N1B4_5705 0.19 -0.3 143,217 - Pf1N1B4_5705 0.30 +2.3 143,303 - Pf1N1B4_5705 0.35 -0.0 143,336 + Pf1N1B4_5705 0.37 -0.3 143,875 + Pf1N1B4_5705 0.65 -1.7 143,876 - Pf1N1B4_5705 0.65 +2.3 143,979 + Pf1N1B4_5705 0.71 -1.6 144,037 + -1.3 144,038 - Pf1N1B4_5705 0.74 +1.3 144,038 - Pf1N1B4_5705 0.74 -0.7 144,180 + -1.9 144,181 - Pf1N1B4_5705 0.81 +2.0 144,278 + Pf1N1B4_5705 0.86 -0.0 144,279 - Pf1N1B4_5705 0.86 +0.5 144,279 - Pf1N1B4_5705 0.86 -0.0 144,303 + Pf1N1B4_5705 0.88 -0.3 144,304 - Pf1N1B4_5705 0.88 -1.9 144,304 - Pf1N1B4_5705 0.88 -0.3 144,362 - -1.3 144,362 - -1.1 144,373 + +0.3 144,373 + -0.3 144,373 + -0.1 144,460 + -0.3 144,461 - +0.1 144,465 - -0.0 144,637 - +0.3 144,884 + Pf1N1B4_5706 0.31 +0.4 144,885 - Pf1N1B4_5706 0.31 -0.3 144,910 + Pf1N1B4_5706 0.33 -2.2 144,978 + Pf1N1B4_5706 0.39 -0.5 145,436 + Pf1N1B4_5706 0.80 +0.1 145,479 - Pf1N1B4_5706 0.84 -1.3 145,479 - Pf1N1B4_5706 0.84 +0.3 145,484 + Pf1N1B4_5706 0.84 +1.7 145,502 + Pf1N1B4_5706 0.86 +0.2
Or see this region's nucleotide sequence