Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5427 and Pf1N1B4_5428 are separated by 474 nucleotides Pf1N1B4_5428 and Pf1N1B4_5429 are separated by 253 nucleotides Pf1N1B4_5429 and Pf1N1B4_5430 are separated by 85 nucleotides
Pf1N1B4_5427 - C4-type zinc finger protein, DksA/TraR family, at 166,711 to 167,184
_5427
Pf1N1B4_5428 - hypothetical protein, at 167,659 to 168,645
_5428
Pf1N1B4_5429 - hypothetical protein, at 168,899 to 169,543
_5429
Pf1N1B4_5430 - hypothetical protein, at 169,629 to 169,796
_5430
Position (kb)
167
168
169 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 166.819 kb on + strand, within Pf1N1B4_5427 at 166.819 kb on + strand, within Pf1N1B4_5427 at 166.820 kb on - strand, within Pf1N1B4_5427 at 166.820 kb on - strand, within Pf1N1B4_5427 at 166.893 kb on - strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.977 kb on - strand at 166.986 kb on + strand, within Pf1N1B4_5427 at 166.986 kb on + strand, within Pf1N1B4_5427 at 166.987 kb on - strand, within Pf1N1B4_5427 at 166.987 kb on - strand, within Pf1N1B4_5427 at 167.056 kb on + strand, within Pf1N1B4_5427 at 167.125 kb on + strand, within Pf1N1B4_5427 at 167.125 kb on + strand, within Pf1N1B4_5427 at 167.125 kb on + strand, within Pf1N1B4_5427 at 167.125 kb on + strand, within Pf1N1B4_5427 at 167.125 kb on + strand, within Pf1N1B4_5427 at 167.125 kb on + strand, within Pf1N1B4_5427 at 167.125 kb on + strand, within Pf1N1B4_5427 at 167.126 kb on - strand, within Pf1N1B4_5427 at 167.128 kb on - strand, within Pf1N1B4_5427 at 167.182 kb on + strand at 167.182 kb on + strand at 167.183 kb on - strand at 167.239 kb on - strand at 167.275 kb on - strand at 167.355 kb on - strand at 167.381 kb on - strand at 167.381 kb on - strand at 167.384 kb on - strand at 167.384 kb on - strand at 167.384 kb on - strand at 167.384 kb on - strand at 167.384 kb on - strand at 167.384 kb on - strand at 167.438 kb on - strand at 167.476 kb on - strand at 167.566 kb on - strand at 167.566 kb on - strand at 167.594 kb on + strand at 167.594 kb on + strand at 167.595 kb on - strand at 167.595 kb on - strand at 167.629 kb on + strand at 167.629 kb on + strand at 167.640 kb on - strand at 167.640 kb on - strand at 167.672 kb on - strand at 167.745 kb on + strand at 167.766 kb on + strand, within Pf1N1B4_5428 at 167.788 kb on + strand, within Pf1N1B4_5428 at 167.788 kb on + strand, within Pf1N1B4_5428 at 167.843 kb on - strand, within Pf1N1B4_5428 at 168.034 kb on + strand, within Pf1N1B4_5428 at 168.035 kb on - strand, within Pf1N1B4_5428 at 168.052 kb on + strand, within Pf1N1B4_5428 at 168.053 kb on - strand, within Pf1N1B4_5428 at 168.053 kb on - strand at 168.069 kb on + strand, within Pf1N1B4_5428 at 168.070 kb on - strand, within Pf1N1B4_5428 at 168.130 kb on + strand, within Pf1N1B4_5428 at 168.131 kb on - strand, within Pf1N1B4_5428 at 168.131 kb on - strand, within Pf1N1B4_5428 at 168.131 kb on - strand, within Pf1N1B4_5428 at 168.131 kb on - strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.134 kb on - strand, within Pf1N1B4_5428 at 168.134 kb on - strand, within Pf1N1B4_5428 at 168.145 kb on + strand, within Pf1N1B4_5428 at 168.196 kb on + strand, within Pf1N1B4_5428 at 168.197 kb on - strand, within Pf1N1B4_5428 at 168.197 kb on - strand, within Pf1N1B4_5428 at 168.197 kb on - strand, within Pf1N1B4_5428 at 168.197 kb on - strand, within Pf1N1B4_5428 at 168.201 kb on - strand, within Pf1N1B4_5428 at 168.381 kb on + strand, within Pf1N1B4_5428 at 168.391 kb on + strand, within Pf1N1B4_5428 at 168.415 kb on + strand, within Pf1N1B4_5428 at 168.415 kb on + strand, within Pf1N1B4_5428 at 168.416 kb on - strand, within Pf1N1B4_5428 at 168.416 kb on - strand, within Pf1N1B4_5428 at 168.419 kb on + strand, within Pf1N1B4_5428 at 168.419 kb on + strand, within Pf1N1B4_5428 at 168.419 kb on + strand, within Pf1N1B4_5428 at 168.419 kb on + strand, within Pf1N1B4_5428 at 168.419 kb on + strand, within Pf1N1B4_5428 at 168.420 kb on - strand, within Pf1N1B4_5428 at 168.524 kb on - strand, within Pf1N1B4_5428 at 168.524 kb on - strand, within Pf1N1B4_5428 at 168.580 kb on + strand at 168.581 kb on - strand at 168.660 kb on - strand at 168.728 kb on + strand at 168.729 kb on - strand at 168.731 kb on + strand at 168.731 kb on + strand at 168.731 kb on + strand at 168.732 kb on - strand at 168.747 kb on + strand at 168.747 kb on + strand at 168.906 kb on - strand at 168.977 kb on + strand, within Pf1N1B4_5429 at 168.978 kb on - strand, within Pf1N1B4_5429 at 169.103 kb on + strand, within Pf1N1B4_5429 at 169.103 kb on + strand, within Pf1N1B4_5429 at 169.175 kb on + strand, within Pf1N1B4_5429 at 169.175 kb on + strand, within Pf1N1B4_5429 at 169.175 kb on + strand, within Pf1N1B4_5429 at 169.175 kb on + strand, within Pf1N1B4_5429 at 169.175 kb on + strand, within Pf1N1B4_5429 at 169.176 kb on - strand, within Pf1N1B4_5429 at 169.304 kb on - strand, within Pf1N1B4_5429 at 169.304 kb on - strand, within Pf1N1B4_5429 at 169.329 kb on + strand, within Pf1N1B4_5429 at 169.337 kb on + strand, within Pf1N1B4_5429 at 169.337 kb on + strand, within Pf1N1B4_5429 at 169.337 kb on + strand, within Pf1N1B4_5429 at 169.338 kb on - strand, within Pf1N1B4_5429 at 169.549 kb on + strand at 169.549 kb on + strand at 169.549 kb on + strand at 169.549 kb on + strand at 169.549 kb on + strand at 169.584 kb on + strand at 169.584 kb on + strand at 169.584 kb on + strand at 169.585 kb on - strand at 169.585 kb on - strand at 169.631 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 166,819 + Pf1N1B4_5427 0.23 -2.1 166,819 + Pf1N1B4_5427 0.23 +0.1 166,820 - Pf1N1B4_5427 0.23 -2.5 166,820 - Pf1N1B4_5427 0.23 -0.7 166,893 - Pf1N1B4_5427 0.38 -1.5 166,976 + Pf1N1B4_5427 0.56 +1.1 166,976 + Pf1N1B4_5427 0.56 -0.5 166,976 + Pf1N1B4_5427 0.56 +0.4 166,976 + Pf1N1B4_5427 0.56 -0.5 166,976 + Pf1N1B4_5427 0.56 -0.2 166,976 + Pf1N1B4_5427 0.56 -0.5 166,976 + Pf1N1B4_5427 0.56 -1.2 166,976 + Pf1N1B4_5427 0.56 +2.1 166,976 + Pf1N1B4_5427 0.56 -0.7 166,977 - +0.1 166,986 + Pf1N1B4_5427 0.58 -1.5 166,986 + Pf1N1B4_5427 0.58 -0.9 166,987 - Pf1N1B4_5427 0.58 +0.1 166,987 - Pf1N1B4_5427 0.58 +1.5 167,056 + Pf1N1B4_5427 0.73 -0.3 167,125 + Pf1N1B4_5427 0.87 -0.1 167,125 + Pf1N1B4_5427 0.87 -0.7 167,125 + Pf1N1B4_5427 0.87 -1.7 167,125 + Pf1N1B4_5427 0.87 +0.1 167,125 + Pf1N1B4_5427 0.87 +1.1 167,125 + Pf1N1B4_5427 0.87 -0.4 167,125 + Pf1N1B4_5427 0.87 -0.9 167,126 - Pf1N1B4_5427 0.88 +0.4 167,128 - Pf1N1B4_5427 0.88 -0.9 167,182 + -0.5 167,182 + +0.6 167,183 - -0.2 167,239 - -0.9 167,275 - -0.3 167,355 - -0.9 167,381 - +1.1 167,381 - +1.6 167,384 - +0.1 167,384 - -0.3 167,384 - +0.3 167,384 - +0.5 167,384 - +0.1 167,384 - +0.1 167,438 - +0.1 167,476 - -2.9 167,566 - +0.1 167,566 - +0.1 167,594 + -0.5 167,594 + -1.5 167,595 - +0.1 167,595 - +1.6 167,629 + +0.1 167,629 + +0.1 167,640 - -2.8 167,640 - -0.1 167,672 - -0.5 167,745 + -0.9 167,766 + Pf1N1B4_5428 0.11 -0.3 167,788 + Pf1N1B4_5428 0.13 +0.1 167,788 + Pf1N1B4_5428 0.13 -1.5 167,843 - Pf1N1B4_5428 0.19 +0.1 168,034 + Pf1N1B4_5428 0.38 +0.9 168,035 - Pf1N1B4_5428 0.38 +0.1 168,052 + Pf1N1B4_5428 0.40 +0.2 168,053 - Pf1N1B4_5428 0.40 -1.5 168,053 - +0.1 168,069 + Pf1N1B4_5428 0.42 +1.1 168,070 - Pf1N1B4_5428 0.42 +0.3 168,130 + Pf1N1B4_5428 0.48 -0.3 168,131 - Pf1N1B4_5428 0.48 -0.5 168,131 - Pf1N1B4_5428 0.48 -0.3 168,131 - Pf1N1B4_5428 0.48 -0.2 168,131 - Pf1N1B4_5428 0.48 -0.4 168,133 + Pf1N1B4_5428 0.48 +0.3 168,133 + Pf1N1B4_5428 0.48 +0.3 168,133 + Pf1N1B4_5428 0.48 -0.4 168,133 + Pf1N1B4_5428 0.48 +1.1 168,133 + Pf1N1B4_5428 0.48 -0.4 168,133 + Pf1N1B4_5428 0.48 -0.3 168,134 - Pf1N1B4_5428 0.48 +1.6 168,134 - Pf1N1B4_5428 0.48 +1.1 168,145 + Pf1N1B4_5428 0.49 +1.4 168,196 + Pf1N1B4_5428 0.54 -1.3 168,197 - Pf1N1B4_5428 0.55 +0.8 168,197 - Pf1N1B4_5428 0.55 +0.6 168,197 - Pf1N1B4_5428 0.55 -1.8 168,197 - Pf1N1B4_5428 0.55 -0.4 168,201 - Pf1N1B4_5428 0.55 +2.1 168,381 + Pf1N1B4_5428 0.73 +0.1 168,391 + Pf1N1B4_5428 0.74 -0.9 168,415 + Pf1N1B4_5428 0.77 +1.2 168,415 + Pf1N1B4_5428 0.77 -0.9 168,416 - Pf1N1B4_5428 0.77 -1.9 168,416 - Pf1N1B4_5428 0.77 +0.6 168,419 + Pf1N1B4_5428 0.77 -1.5 168,419 + Pf1N1B4_5428 0.77 +0.1 168,419 + Pf1N1B4_5428 0.77 +0.1 168,419 + Pf1N1B4_5428 0.77 -0.9 168,419 + Pf1N1B4_5428 0.77 +0.4 168,420 - Pf1N1B4_5428 0.77 +0.1 168,524 - Pf1N1B4_5428 0.88 +0.1 168,524 - Pf1N1B4_5428 0.88 +0.1 168,580 + -1.5 168,581 - -2.3 168,660 - -0.9 168,728 + -0.9 168,729 - -0.5 168,731 + -1.9 168,731 + +0.9 168,731 + +0.3 168,732 - +1.6 168,747 + +0.1 168,747 + -0.9 168,906 - +1.1 168,977 + Pf1N1B4_5429 0.12 -0.2 168,978 - Pf1N1B4_5429 0.12 +0.6 169,103 + Pf1N1B4_5429 0.32 -0.7 169,103 + Pf1N1B4_5429 0.32 -0.4 169,175 + Pf1N1B4_5429 0.43 -0.8 169,175 + Pf1N1B4_5429 0.43 -0.9 169,175 + Pf1N1B4_5429 0.43 +1.1 169,175 + Pf1N1B4_5429 0.43 -1.9 169,175 + Pf1N1B4_5429 0.43 +0.5 169,176 - Pf1N1B4_5429 0.43 +1.6 169,304 - Pf1N1B4_5429 0.63 -0.7 169,304 - Pf1N1B4_5429 0.63 -0.3 169,329 + Pf1N1B4_5429 0.67 -1.3 169,337 + Pf1N1B4_5429 0.68 +1.6 169,337 + Pf1N1B4_5429 0.68 -0.4 169,337 + Pf1N1B4_5429 0.68 +1.5 169,338 - Pf1N1B4_5429 0.68 -0.4 169,549 + -0.2 169,549 + +2.4 169,549 + +0.4 169,549 + -0.4 169,549 + +0.7 169,584 + -1.5 169,584 + +1.6 169,584 + -0.1 169,585 - -1.5 169,585 - -1.3 169,631 - -1.7
Or see this region's nucleotide sequence