Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4995 and Pf1N1B4_4996 are separated by 107 nucleotides Pf1N1B4_4996 and Pf1N1B4_4997 are separated by 40 nucleotides
Pf1N1B4_4995 - Glutamate Aspartate periplasmic binding protein precursor GltI (TC 3.A.1.3.4), at 5,542,380 to 5,543,303
_4995
Pf1N1B4_4996 - Aspartate aminotransferase (EC 2.6.1.1), at 5,543,411 to 5,544,598
_4996
Pf1N1B4_4997 - Methyl-accepting chemotaxis protein, at 5,544,639 to 5,546,264
_4997
Position (kb)
5543
5544
5545 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5542.502 kb on + strand, within Pf1N1B4_4995 at 5542.503 kb on - strand at 5542.576 kb on - strand, within Pf1N1B4_4995 at 5542.773 kb on + strand, within Pf1N1B4_4995 at 5542.774 kb on - strand, within Pf1N1B4_4995 at 5542.774 kb on - strand at 5542.831 kb on + strand, within Pf1N1B4_4995 at 5542.832 kb on - strand at 5542.832 kb on - strand, within Pf1N1B4_4995 at 5542.832 kb on - strand, within Pf1N1B4_4995 at 5542.836 kb on - strand at 5542.906 kb on - strand, within Pf1N1B4_4995 at 5543.089 kb on - strand, within Pf1N1B4_4995 at 5543.235 kb on + strand at 5543.235 kb on + strand at 5543.245 kb on - strand at 5543.289 kb on + strand at 5543.510 kb on - strand at 5543.759 kb on + strand, within Pf1N1B4_4996 at 5543.979 kb on + strand, within Pf1N1B4_4996 at 5544.213 kb on + strand, within Pf1N1B4_4996 at 5544.214 kb on - strand, within Pf1N1B4_4996 at 5544.214 kb on - strand, within Pf1N1B4_4996 at 5544.214 kb on - strand, within Pf1N1B4_4996 at 5544.214 kb on - strand, within Pf1N1B4_4996 at 5544.222 kb on + strand, within Pf1N1B4_4996 at 5544.222 kb on + strand, within Pf1N1B4_4996 at 5544.222 kb on + strand, within Pf1N1B4_4996 at 5544.222 kb on + strand, within Pf1N1B4_4996 at 5544.222 kb on + strand, within Pf1N1B4_4996 at 5544.223 kb on - strand, within Pf1N1B4_4996 at 5544.223 kb on - strand, within Pf1N1B4_4996 at 5544.223 kb on - strand, within Pf1N1B4_4996 at 5544.223 kb on - strand, within Pf1N1B4_4996 at 5544.283 kb on - strand, within Pf1N1B4_4996 at 5544.370 kb on + strand, within Pf1N1B4_4996 at 5544.371 kb on - strand, within Pf1N1B4_4996 at 5544.371 kb on - strand at 5544.396 kb on - strand, within Pf1N1B4_4996 at 5544.600 kb on + strand at 5544.600 kb on + strand at 5544.600 kb on + strand at 5544.636 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 5,542,502 + Pf1N1B4_4995 0.13 -0.0 5,542,503 - +0.6 5,542,576 - Pf1N1B4_4995 0.21 +0.6 5,542,773 + Pf1N1B4_4995 0.43 +1.1 5,542,774 - Pf1N1B4_4995 0.43 -0.4 5,542,774 - -1.4 5,542,831 + Pf1N1B4_4995 0.49 +0.6 5,542,832 - -0.0 5,542,832 - Pf1N1B4_4995 0.49 +0.2 5,542,832 - Pf1N1B4_4995 0.49 +2.4 5,542,836 - +1.9 5,542,906 - Pf1N1B4_4995 0.57 -2.4 5,543,089 - Pf1N1B4_4995 0.77 -0.7 5,543,235 + +0.4 5,543,235 + +1.6 5,543,245 - -0.4 5,543,289 + +0.6 5,543,510 - +0.8 5,543,759 + Pf1N1B4_4996 0.29 +1.6 5,543,979 + Pf1N1B4_4996 0.48 +0.1 5,544,213 + Pf1N1B4_4996 0.68 +2.4 5,544,214 - Pf1N1B4_4996 0.68 +0.1 5,544,214 - Pf1N1B4_4996 0.68 -0.7 5,544,214 - Pf1N1B4_4996 0.68 -1.4 5,544,214 - Pf1N1B4_4996 0.68 +0.1 5,544,222 + Pf1N1B4_4996 0.68 +0.9 5,544,222 + Pf1N1B4_4996 0.68 -1.0 5,544,222 + Pf1N1B4_4996 0.68 -0.8 5,544,222 + Pf1N1B4_4996 0.68 +1.6 5,544,222 + Pf1N1B4_4996 0.68 -0.0 5,544,223 - Pf1N1B4_4996 0.68 -0.9 5,544,223 - Pf1N1B4_4996 0.68 +0.1 5,544,223 - Pf1N1B4_4996 0.68 -0.1 5,544,223 - Pf1N1B4_4996 0.68 +0.1 5,544,283 - Pf1N1B4_4996 0.73 +0.1 5,544,370 + Pf1N1B4_4996 0.81 -0.0 5,544,371 - Pf1N1B4_4996 0.81 -0.8 5,544,371 - -0.4 5,544,396 - Pf1N1B4_4996 0.83 +1.1 5,544,600 + -0.4 5,544,600 + -1.4 5,544,600 + -0.4 5,544,636 - -0.4
Or see this region's nucleotide sequence