Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4424 and Pf1N1B4_4425 overlap by 4 nucleotides Pf1N1B4_4425 and Pf1N1B4_4426 overlap by 4 nucleotides Pf1N1B4_4426 and Pf1N1B4_4427 overlap by 11 nucleotides
Pf1N1B4_4424 - D-2-hydroxyglutarate dehydrogenase, at 4,884,343 to 4,885,767
_4424
Pf1N1B4_4425 - Aspartate aminotransferase (EC 2.6.1.1), at 4,885,764 to 4,886,975
_4425
Pf1N1B4_4426 - Glutamate transport membrane-spanning protein, at 4,886,972 to 4,887,706
_4426
Pf1N1B4_4427 - Glutamate transport membrane-spanning protein, at 4,887,696 to 4,888,484
_4427
Position (kb)
4885
4886
4887 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4885.471 kb on + strand, within Pf1N1B4_4424 at 4885.472 kb on - strand, within Pf1N1B4_4424 at 4885.507 kb on + strand, within Pf1N1B4_4424 at 4885.507 kb on + strand at 4885.508 kb on - strand, within Pf1N1B4_4424 at 4885.665 kb on + strand at 4885.666 kb on - strand at 4885.666 kb on - strand at 4885.666 kb on - strand at 4885.745 kb on + strand at 4885.926 kb on + strand, within Pf1N1B4_4425 at 4885.956 kb on + strand, within Pf1N1B4_4425 at 4886.032 kb on - strand, within Pf1N1B4_4425 at 4886.244 kb on + strand at 4886.244 kb on + strand, within Pf1N1B4_4425 at 4886.245 kb on - strand, within Pf1N1B4_4425 at 4886.245 kb on - strand, within Pf1N1B4_4425 at 4886.305 kb on + strand at 4886.308 kb on - strand, within Pf1N1B4_4425 at 4886.308 kb on - strand, within Pf1N1B4_4425 at 4886.487 kb on + strand, within Pf1N1B4_4425 at 4886.488 kb on - strand, within Pf1N1B4_4425 at 4886.678 kb on - strand, within Pf1N1B4_4425 at 4886.744 kb on - strand, within Pf1N1B4_4425 at 4886.748 kb on - strand, within Pf1N1B4_4425 at 4887.026 kb on + strand at 4887.558 kb on - strand, within Pf1N1B4_4426
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 4,885,471 + Pf1N1B4_4424 0.79 +0.6 4,885,472 - Pf1N1B4_4424 0.79 +0.2 4,885,507 + Pf1N1B4_4424 0.82 +0.6 4,885,507 + -1.4 4,885,508 - Pf1N1B4_4424 0.82 -0.6 4,885,665 + +0.2 4,885,666 - +0.6 4,885,666 - +0.6 4,885,666 - +2.9 4,885,745 + +0.2 4,885,926 + Pf1N1B4_4425 0.13 +0.2 4,885,956 + Pf1N1B4_4425 0.16 -1.4 4,886,032 - Pf1N1B4_4425 0.22 -1.0 4,886,244 + -2.0 4,886,244 + Pf1N1B4_4425 0.40 +0.4 4,886,245 - Pf1N1B4_4425 0.40 +0.6 4,886,245 - Pf1N1B4_4425 0.40 +0.8 4,886,305 + -0.4 4,886,308 - Pf1N1B4_4425 0.45 -0.8 4,886,308 - Pf1N1B4_4425 0.45 +1.2 4,886,487 + Pf1N1B4_4425 0.60 -0.4 4,886,488 - Pf1N1B4_4425 0.60 -0.9 4,886,678 - Pf1N1B4_4425 0.75 -0.1 4,886,744 - Pf1N1B4_4425 0.81 -1.0 4,886,748 - Pf1N1B4_4425 0.81 -0.4 4,887,026 + +0.6 4,887,558 - Pf1N1B4_4426 0.80 +1.6
Or see this region's nucleotide sequence