Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4185 and Pf1N1B4_4186 are separated by 88 nucleotides Pf1N1B4_4186 and Pf1N1B4_4187 are separated by 315 nucleotides Pf1N1B4_4187 and Pf1N1B4_4188 are separated by 59 nucleotides
Pf1N1B4_4185 - Permease of the drug/metabolite transporter (DMT) superfamily, at 4,633,712 to 4,634,608
_4185
Pf1N1B4_4186 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 4,634,697 to 4,636,028
_4186
Pf1N1B4_4187 - Probable signal peptide protein, at 4,636,344 to 4,636,913
_4187
Pf1N1B4_4188 - acetyltransferase, GNAT family, at 4,636,973 to 4,637,683
_4188
Position (kb)
4634
4635
4636
4637 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4633.714 kb on - strand at 4633.835 kb on + strand, within Pf1N1B4_4185 at 4633.836 kb on - strand, within Pf1N1B4_4185 at 4633.929 kb on + strand, within Pf1N1B4_4185 at 4633.930 kb on - strand, within Pf1N1B4_4185 at 4633.930 kb on - strand, within Pf1N1B4_4185 at 4634.308 kb on - strand, within Pf1N1B4_4185 at 4634.308 kb on - strand, within Pf1N1B4_4185 at 4634.308 kb on - strand, within Pf1N1B4_4185 at 4634.308 kb on - strand, within Pf1N1B4_4185 at 4634.345 kb on + strand, within Pf1N1B4_4185 at 4634.345 kb on + strand at 4634.345 kb on + strand, within Pf1N1B4_4185 at 4634.346 kb on - strand, within Pf1N1B4_4185 at 4634.647 kb on + strand at 4634.647 kb on + strand at 4634.664 kb on - strand at 4634.842 kb on - strand, within Pf1N1B4_4186 at 4634.930 kb on + strand, within Pf1N1B4_4186 at 4634.931 kb on - strand, within Pf1N1B4_4186 at 4634.942 kb on + strand, within Pf1N1B4_4186 at 4634.943 kb on - strand, within Pf1N1B4_4186 at 4634.980 kb on - strand, within Pf1N1B4_4186 at 4635.246 kb on + strand, within Pf1N1B4_4186 at 4635.246 kb on + strand at 4635.247 kb on - strand at 4635.247 kb on - strand, within Pf1N1B4_4186 at 4635.372 kb on + strand, within Pf1N1B4_4186 at 4635.487 kb on - strand, within Pf1N1B4_4186 at 4635.549 kb on + strand, within Pf1N1B4_4186 at 4635.549 kb on + strand, within Pf1N1B4_4186 at 4635.550 kb on - strand, within Pf1N1B4_4186 at 4635.550 kb on - strand, within Pf1N1B4_4186 at 4635.553 kb on + strand, within Pf1N1B4_4186 at 4635.553 kb on + strand, within Pf1N1B4_4186 at 4635.778 kb on + strand, within Pf1N1B4_4186 at 4636.030 kb on - strand at 4636.224 kb on + strand at 4636.266 kb on - strand at 4636.273 kb on + strand at 4636.426 kb on - strand, within Pf1N1B4_4187 at 4636.467 kb on + strand, within Pf1N1B4_4187 at 4636.468 kb on - strand, within Pf1N1B4_4187 at 4636.468 kb on - strand, within Pf1N1B4_4187 at 4636.468 kb on - strand at 4636.468 kb on - strand, within Pf1N1B4_4187 at 4636.468 kb on - strand, within Pf1N1B4_4187 at 4636.762 kb on + strand, within Pf1N1B4_4187 at 4636.762 kb on + strand, within Pf1N1B4_4187 at 4636.762 kb on + strand, within Pf1N1B4_4187 at 4636.762 kb on + strand, within Pf1N1B4_4187 at 4636.763 kb on - strand, within Pf1N1B4_4187 at 4636.763 kb on - strand at 4636.763 kb on - strand, within Pf1N1B4_4187 at 4636.964 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 4,633,714 - -0.3 4,633,835 + Pf1N1B4_4185 0.14 +0.9 4,633,836 - Pf1N1B4_4185 0.14 +0.1 4,633,929 + Pf1N1B4_4185 0.24 +0.1 4,633,930 - Pf1N1B4_4185 0.24 +1.2 4,633,930 - Pf1N1B4_4185 0.24 -0.3 4,634,308 - Pf1N1B4_4185 0.66 -1.9 4,634,308 - Pf1N1B4_4185 0.66 -0.3 4,634,308 - Pf1N1B4_4185 0.66 -0.3 4,634,308 - Pf1N1B4_4185 0.66 -0.2 4,634,345 + Pf1N1B4_4185 0.71 -0.3 4,634,345 + +0.7 4,634,345 + Pf1N1B4_4185 0.71 +1.7 4,634,346 - Pf1N1B4_4185 0.71 +0.3 4,634,647 + -0.3 4,634,647 + +1.2 4,634,664 - +2.0 4,634,842 - Pf1N1B4_4186 0.11 -1.1 4,634,930 + Pf1N1B4_4186 0.17 -0.8 4,634,931 - Pf1N1B4_4186 0.18 -1.2 4,634,942 + Pf1N1B4_4186 0.18 +0.4 4,634,943 - Pf1N1B4_4186 0.18 -1.1 4,634,980 - Pf1N1B4_4186 0.21 -1.9 4,635,246 + Pf1N1B4_4186 0.41 -0.8 4,635,246 + -0.3 4,635,247 - -0.9 4,635,247 - Pf1N1B4_4186 0.41 -1.1 4,635,372 + Pf1N1B4_4186 0.51 +0.2 4,635,487 - Pf1N1B4_4186 0.59 -0.3 4,635,549 + Pf1N1B4_4186 0.64 -0.1 4,635,549 + Pf1N1B4_4186 0.64 +0.5 4,635,550 - Pf1N1B4_4186 0.64 +1.8 4,635,550 - Pf1N1B4_4186 0.64 -0.3 4,635,553 + Pf1N1B4_4186 0.64 -0.3 4,635,553 + Pf1N1B4_4186 0.64 -0.3 4,635,778 + Pf1N1B4_4186 0.81 -0.3 4,636,030 - -3.2 4,636,224 + -1.4 4,636,266 - -1.0 4,636,273 + +2.0 4,636,426 - Pf1N1B4_4187 0.14 +0.4 4,636,467 + Pf1N1B4_4187 0.22 -0.4 4,636,468 - Pf1N1B4_4187 0.22 +0.2 4,636,468 - Pf1N1B4_4187 0.22 +1.2 4,636,468 - +1.2 4,636,468 - Pf1N1B4_4187 0.22 -1.4 4,636,468 - Pf1N1B4_4187 0.22 +0.6 4,636,762 + Pf1N1B4_4187 0.73 -1.7 4,636,762 + Pf1N1B4_4187 0.73 +0.1 4,636,762 + Pf1N1B4_4187 0.73 -0.4 4,636,762 + Pf1N1B4_4187 0.73 -0.4 4,636,763 - Pf1N1B4_4187 0.74 -1.4 4,636,763 - +0.6 4,636,763 - Pf1N1B4_4187 0.74 -1.8 4,636,964 + -0.0
Or see this region's nucleotide sequence