Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4034 and Pf1N1B4_4035 overlap by 4 nucleotides Pf1N1B4_4035 and Pf1N1B4_4036 overlap by 78 nucleotides Pf1N1B4_4036 and Pf1N1B4_4037 are separated by 328 nucleotides
Pf1N1B4_4034 - NADH-ubiquinone oxidoreductase chain H (EC 1.6.5.3), at 4,484,527 to 4,485,534
_4034
Pf1N1B4_4035 - NADH-ubiquinone oxidoreductase chain G (EC 1.6.5.3), at 4,485,531 to 4,487,990
_4035
Pf1N1B4_4036 - hypothetical protein, at 4,487,913 to 4,488,056
_4036
Pf1N1B4_4037 - NADH-ubiquinone oxidoreductase chain F (EC 1.6.5.3), at 4,488,385 to 4,489,740
_4037
Position (kb)
4485
4486
4487
4488 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4484.807 kb on + strand, within Pf1N1B4_4034 at 4485.124 kb on - strand, within Pf1N1B4_4034 at 4485.503 kb on - strand at 4485.742 kb on + strand at 4485.743 kb on - strand at 4485.743 kb on - strand at 4485.743 kb on - strand at 4485.743 kb on - strand at 4486.005 kb on + strand, within Pf1N1B4_4035 at 4487.245 kb on + strand, within Pf1N1B4_4035 at 4487.246 kb on - strand, within Pf1N1B4_4035 at 4487.468 kb on - strand, within Pf1N1B4_4035 at 4487.707 kb on + strand, within Pf1N1B4_4035 at 4487.708 kb on - strand, within Pf1N1B4_4035 at 4487.756 kb on - strand at 4487.765 kb on - strand at 4488.152 kb on + strand at 4488.239 kb on - strand at 4488.330 kb on - strand at 4488.379 kb on - strand at 4488.379 kb on - strand at 4488.379 kb on - strand at 4488.471 kb on - strand at 4488.782 kb on + strand, within Pf1N1B4_4037 at 4488.783 kb on - strand, within Pf1N1B4_4037 at 4488.783 kb on - strand, within Pf1N1B4_4037
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 4,484,807 + Pf1N1B4_4034 0.28 -1.6 4,485,124 - Pf1N1B4_4034 0.59 -2.5 4,485,503 - -2.5 4,485,742 + -1.8 4,485,743 - -0.2 4,485,743 - +0.4 4,485,743 - -1.8 4,485,743 - -0.8 4,486,005 + Pf1N1B4_4035 0.19 -0.2 4,487,245 + Pf1N1B4_4035 0.70 -1.5 4,487,246 - Pf1N1B4_4035 0.70 -1.8 4,487,468 - Pf1N1B4_4035 0.79 -1.5 4,487,707 + Pf1N1B4_4035 0.88 +0.8 4,487,708 - Pf1N1B4_4035 0.88 +0.8 4,487,756 - -2.2 4,487,765 - -4.4 4,488,152 + -2.2 4,488,239 - -1.2 4,488,330 - -0.8 4,488,379 - +0.0 4,488,379 - +0.0 4,488,379 - -0.2 4,488,471 - -3.2 4,488,782 + Pf1N1B4_4037 0.29 +0.8 4,488,783 - Pf1N1B4_4037 0.29 -1.2 4,488,783 - Pf1N1B4_4037 0.29 -3.4
Or see this region's nucleotide sequence