Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_384 and Pf1N1B4_385 are separated by 158 nucleotides Pf1N1B4_385 and Pf1N1B4_386 are separated by 87 nucleotides Pf1N1B4_386 and Pf1N1B4_387 overlap by 24 nucleotides Pf1N1B4_387 and Pf1N1B4_388 are separated by 120 nucleotides
Pf1N1B4_384 - proteinase inhibitor, at 454,198 to 454,452
_384
Pf1N1B4_385 - Translation initiation factor 2 (IF-2; GTPase), at 454,611 to 455,078
_385
Pf1N1B4_386 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 455,166 to 456,605
_386
Pf1N1B4_387 - hypothetical protein, at 456,582 to 456,695
_387
Pf1N1B4_388 - OmpA family protein, at 456,816 to 457,628
_388
Position (kb)
455
456
457 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 454.342 kb on - strand, within Pf1N1B4_384 at 454.351 kb on + strand, within Pf1N1B4_384 at 454.351 kb on + strand, within Pf1N1B4_384 at 454.352 kb on - strand, within Pf1N1B4_384 at 454.352 kb on - strand, within Pf1N1B4_384 at 454.516 kb on + strand at 454.613 kb on - strand at 454.819 kb on - strand, within Pf1N1B4_385 at 455.034 kb on + strand at 455.107 kb on + strand at 455.107 kb on + strand at 455.108 kb on - strand at 455.108 kb on - strand at 455.108 kb on - strand at 455.108 kb on - strand at 455.108 kb on - strand at 455.108 kb on - strand at 455.109 kb on + strand at 455.109 kb on + strand at 455.109 kb on + strand at 455.109 kb on + strand at 455.110 kb on - strand at 455.110 kb on - strand at 455.110 kb on - strand at 455.110 kb on - strand at 455.440 kb on + strand, within Pf1N1B4_386 at 455.440 kb on + strand, within Pf1N1B4_386 at 455.440 kb on + strand, within Pf1N1B4_386 at 455.440 kb on + strand, within Pf1N1B4_386 at 455.440 kb on + strand, within Pf1N1B4_386 at 455.440 kb on + strand, within Pf1N1B4_386 at 455.440 kb on + strand, within Pf1N1B4_386 at 455.441 kb on - strand at 455.441 kb on - strand, within Pf1N1B4_386 at 455.441 kb on - strand, within Pf1N1B4_386 at 455.441 kb on - strand, within Pf1N1B4_386 at 455.482 kb on + strand, within Pf1N1B4_386 at 455.534 kb on - strand, within Pf1N1B4_386 at 455.611 kb on + strand, within Pf1N1B4_386 at 455.611 kb on + strand at 455.611 kb on + strand, within Pf1N1B4_386 at 455.611 kb on + strand, within Pf1N1B4_386 at 455.611 kb on + strand, within Pf1N1B4_386 at 455.612 kb on - strand, within Pf1N1B4_386 at 455.612 kb on - strand, within Pf1N1B4_386 at 455.612 kb on - strand, within Pf1N1B4_386 at 455.612 kb on - strand, within Pf1N1B4_386 at 455.677 kb on + strand, within Pf1N1B4_386 at 455.677 kb on + strand, within Pf1N1B4_386 at 455.677 kb on + strand, within Pf1N1B4_386 at 455.677 kb on + strand, within Pf1N1B4_386 at 455.677 kb on + strand, within Pf1N1B4_386 at 455.677 kb on + strand, within Pf1N1B4_386 at 455.677 kb on + strand, within Pf1N1B4_386 at 455.677 kb on + strand, within Pf1N1B4_386 at 455.677 kb on + strand, within Pf1N1B4_386 at 455.678 kb on - strand, within Pf1N1B4_386 at 455.678 kb on - strand, within Pf1N1B4_386 at 455.678 kb on - strand, within Pf1N1B4_386 at 455.678 kb on - strand, within Pf1N1B4_386 at 455.678 kb on - strand, within Pf1N1B4_386 at 455.678 kb on - strand, within Pf1N1B4_386 at 455.678 kb on - strand, within Pf1N1B4_386 at 455.678 kb on - strand, within Pf1N1B4_386 at 455.701 kb on + strand, within Pf1N1B4_386 at 455.750 kb on - strand, within Pf1N1B4_386 at 455.750 kb on - strand, within Pf1N1B4_386 at 455.753 kb on + strand, within Pf1N1B4_386 at 455.754 kb on - strand, within Pf1N1B4_386 at 455.754 kb on - strand, within Pf1N1B4_386 at 455.754 kb on - strand, within Pf1N1B4_386 at 455.844 kb on - strand, within Pf1N1B4_386 at 455.844 kb on - strand, within Pf1N1B4_386 at 455.989 kb on + strand, within Pf1N1B4_386 at 455.992 kb on + strand, within Pf1N1B4_386 at 455.992 kb on + strand, within Pf1N1B4_386 at 455.992 kb on + strand, within Pf1N1B4_386 at 455.992 kb on + strand at 455.992 kb on + strand, within Pf1N1B4_386 at 455.992 kb on + strand, within Pf1N1B4_386 at 455.992 kb on + strand, within Pf1N1B4_386 at 455.992 kb on + strand, within Pf1N1B4_386 at 455.992 kb on + strand, within Pf1N1B4_386 at 455.992 kb on + strand, within Pf1N1B4_386 at 456.036 kb on + strand, within Pf1N1B4_386 at 456.172 kb on + strand, within Pf1N1B4_386 at 456.172 kb on + strand, within Pf1N1B4_386 at 456.172 kb on + strand, within Pf1N1B4_386 at 456.172 kb on + strand at 456.172 kb on + strand, within Pf1N1B4_386 at 456.172 kb on + strand, within Pf1N1B4_386 at 456.172 kb on + strand, within Pf1N1B4_386 at 456.173 kb on - strand, within Pf1N1B4_386 at 456.173 kb on - strand, within Pf1N1B4_386 at 456.173 kb on - strand, within Pf1N1B4_386 at 456.173 kb on - strand, within Pf1N1B4_386 at 456.173 kb on - strand, within Pf1N1B4_386 at 456.245 kb on - strand, within Pf1N1B4_386 at 456.245 kb on - strand, within Pf1N1B4_386 at 456.245 kb on - strand, within Pf1N1B4_386 at 456.245 kb on - strand, within Pf1N1B4_386 at 456.403 kb on + strand, within Pf1N1B4_386 at 456.403 kb on + strand, within Pf1N1B4_386 at 456.403 kb on + strand, within Pf1N1B4_386 at 456.403 kb on + strand, within Pf1N1B4_386 at 456.403 kb on + strand, within Pf1N1B4_386 at 456.403 kb on + strand, within Pf1N1B4_386 at 456.404 kb on - strand, within Pf1N1B4_386 at 456.404 kb on - strand, within Pf1N1B4_386 at 456.538 kb on + strand at 456.538 kb on + strand at 456.539 kb on - strand at 456.539 kb on - strand at 456.578 kb on + strand at 456.578 kb on + strand at 456.578 kb on + strand at 456.578 kb on + strand at 456.579 kb on - strand at 456.579 kb on - strand at 456.579 kb on - strand at 456.579 kb on - strand at 456.586 kb on + strand at 456.586 kb on + strand at 456.586 kb on + strand at 456.586 kb on + strand at 456.634 kb on + strand, within Pf1N1B4_387 at 456.634 kb on + strand, within Pf1N1B4_387 at 456.634 kb on + strand, within Pf1N1B4_387 at 456.635 kb on - strand, within Pf1N1B4_387 at 456.665 kb on + strand, within Pf1N1B4_387 at 456.673 kb on + strand, within Pf1N1B4_387 at 456.703 kb on + strand at 456.703 kb on + strand at 456.703 kb on + strand at 456.703 kb on + strand at 456.944 kb on + strand at 456.944 kb on + strand, within Pf1N1B4_388 at 456.945 kb on - strand, within Pf1N1B4_388 at 456.945 kb on - strand, within Pf1N1B4_388 at 456.945 kb on - strand, within Pf1N1B4_388 at 456.945 kb on - strand, within Pf1N1B4_388 at 456.945 kb on - strand at 456.945 kb on - strand, within Pf1N1B4_388 at 456.945 kb on - strand, within Pf1N1B4_388 at 456.945 kb on - strand, within Pf1N1B4_388 at 457.048 kb on + strand, within Pf1N1B4_388 at 457.049 kb on - strand, within Pf1N1B4_388 at 457.049 kb on - strand, within Pf1N1B4_388 at 457.366 kb on + strand, within Pf1N1B4_388 at 457.367 kb on - strand, within Pf1N1B4_388 at 457.381 kb on + strand, within Pf1N1B4_388 at 457.381 kb on + strand, within Pf1N1B4_388 at 457.381 kb on + strand, within Pf1N1B4_388 at 457.381 kb on + strand, within Pf1N1B4_388 at 457.381 kb on + strand, within Pf1N1B4_388 at 457.382 kb on - strand, within Pf1N1B4_388 at 457.387 kb on + strand, within Pf1N1B4_388 at 457.388 kb on - strand, within Pf1N1B4_388 at 457.388 kb on - strand, within Pf1N1B4_388 at 457.418 kb on - strand, within Pf1N1B4_388 at 457.418 kb on - strand, within Pf1N1B4_388 at 457.571 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 454,342 - Pf1N1B4_384 0.56 -1.1 454,351 + Pf1N1B4_384 0.60 -0.1 454,351 + Pf1N1B4_384 0.60 +0.9 454,352 - Pf1N1B4_384 0.60 -0.4 454,352 - Pf1N1B4_384 0.60 -1.1 454,516 + -1.4 454,613 - -0.7 454,819 - Pf1N1B4_385 0.44 +0.7 455,034 + +0.3 455,107 + -0.5 455,107 + +1.0 455,108 - +0.4 455,108 - +0.3 455,108 - -0.1 455,108 - +0.4 455,108 - +0.9 455,108 - -1.1 455,109 + +2.4 455,109 + -0.5 455,109 + -0.7 455,109 + -0.9 455,110 - -0.1 455,110 - -0.6 455,110 - +1.4 455,110 - -0.1 455,440 + Pf1N1B4_386 0.19 +0.4 455,440 + Pf1N1B4_386 0.19 +3.0 455,440 + Pf1N1B4_386 0.19 +1.2 455,440 + Pf1N1B4_386 0.19 +2.9 455,440 + Pf1N1B4_386 0.19 +1.2 455,440 + Pf1N1B4_386 0.19 -0.2 455,440 + Pf1N1B4_386 0.19 +0.3 455,441 - +0.4 455,441 - Pf1N1B4_386 0.19 +1.1 455,441 - Pf1N1B4_386 0.19 +2.2 455,441 - Pf1N1B4_386 0.19 +0.9 455,482 + Pf1N1B4_386 0.22 +1.1 455,534 - Pf1N1B4_386 0.26 +1.4 455,611 + Pf1N1B4_386 0.31 +1.1 455,611 + +1.1 455,611 + Pf1N1B4_386 0.31 +2.3 455,611 + Pf1N1B4_386 0.31 +0.6 455,611 + Pf1N1B4_386 0.31 +1.2 455,612 - Pf1N1B4_386 0.31 +1.4 455,612 - Pf1N1B4_386 0.31 +1.4 455,612 - Pf1N1B4_386 0.31 -0.1 455,612 - Pf1N1B4_386 0.31 +2.6 455,677 + Pf1N1B4_386 0.35 -0.1 455,677 + Pf1N1B4_386 0.35 +1.0 455,677 + Pf1N1B4_386 0.35 -0.7 455,677 + Pf1N1B4_386 0.35 -0.4 455,677 + Pf1N1B4_386 0.35 +2.7 455,677 + Pf1N1B4_386 0.35 +1.4 455,677 + Pf1N1B4_386 0.35 +0.4 455,677 + Pf1N1B4_386 0.35 +0.9 455,677 + Pf1N1B4_386 0.35 +1.4 455,678 - Pf1N1B4_386 0.36 -2.1 455,678 - Pf1N1B4_386 0.36 +0.9 455,678 - Pf1N1B4_386 0.36 +0.9 455,678 - Pf1N1B4_386 0.36 +0.5 455,678 - Pf1N1B4_386 0.36 +1.2 455,678 - Pf1N1B4_386 0.36 +2.2 455,678 - Pf1N1B4_386 0.36 +1.4 455,678 - Pf1N1B4_386 0.36 +1.9 455,701 + Pf1N1B4_386 0.37 -0.7 455,750 - Pf1N1B4_386 0.41 -0.1 455,750 - Pf1N1B4_386 0.41 +1.4 455,753 + Pf1N1B4_386 0.41 +1.1 455,754 - Pf1N1B4_386 0.41 +0.7 455,754 - Pf1N1B4_386 0.41 +0.4 455,754 - Pf1N1B4_386 0.41 -0.1 455,844 - Pf1N1B4_386 0.47 +0.4 455,844 - Pf1N1B4_386 0.47 +1.9 455,989 + Pf1N1B4_386 0.57 +2.2 455,992 + Pf1N1B4_386 0.57 +0.2 455,992 + Pf1N1B4_386 0.57 +1.9 455,992 + Pf1N1B4_386 0.57 +1.9 455,992 + +0.4 455,992 + Pf1N1B4_386 0.57 -0.1 455,992 + Pf1N1B4_386 0.57 +0.1 455,992 + Pf1N1B4_386 0.57 +1.0 455,992 + Pf1N1B4_386 0.57 +0.6 455,992 + Pf1N1B4_386 0.57 -0.7 455,992 + Pf1N1B4_386 0.57 +0.8 456,036 + Pf1N1B4_386 0.60 +1.3 456,172 + Pf1N1B4_386 0.70 +0.9 456,172 + Pf1N1B4_386 0.70 -0.1 456,172 + Pf1N1B4_386 0.70 +1.3 456,172 + +1.4 456,172 + Pf1N1B4_386 0.70 +2.0 456,172 + Pf1N1B4_386 0.70 +2.0 456,172 + Pf1N1B4_386 0.70 +0.6 456,173 - Pf1N1B4_386 0.70 +0.5 456,173 - Pf1N1B4_386 0.70 +2.9 456,173 - Pf1N1B4_386 0.70 +2.3 456,173 - Pf1N1B4_386 0.70 +1.0 456,173 - Pf1N1B4_386 0.70 +1.2 456,245 - Pf1N1B4_386 0.75 +1.1 456,245 - Pf1N1B4_386 0.75 +0.9 456,245 - Pf1N1B4_386 0.75 +1.2 456,245 - Pf1N1B4_386 0.75 +0.6 456,403 + Pf1N1B4_386 0.86 -1.1 456,403 + Pf1N1B4_386 0.86 +1.7 456,403 + Pf1N1B4_386 0.86 +1.2 456,403 + Pf1N1B4_386 0.86 +1.8 456,403 + Pf1N1B4_386 0.86 -0.1 456,403 + Pf1N1B4_386 0.86 +0.6 456,404 - Pf1N1B4_386 0.86 +0.4 456,404 - Pf1N1B4_386 0.86 +1.7 456,538 + +1.2 456,538 + +1.3 456,539 - +0.6 456,539 - +1.3 456,578 + -0.1 456,578 + +1.0 456,578 + +0.4 456,578 + +0.6 456,579 - +0.4 456,579 - +0.9 456,579 - +1.9 456,579 - +2.2 456,586 + -1.1 456,586 + +1.3 456,586 + +2.0 456,586 + +1.4 456,634 + Pf1N1B4_387 0.46 +2.9 456,634 + Pf1N1B4_387 0.46 +3.0 456,634 + Pf1N1B4_387 0.46 +1.0 456,635 - Pf1N1B4_387 0.46 -2.1 456,665 + Pf1N1B4_387 0.73 +0.1 456,673 + Pf1N1B4_387 0.80 +0.4 456,703 + -0.4 456,703 + +0.9 456,703 + -1.1 456,703 + -0.6 456,944 + +0.9 456,944 + Pf1N1B4_388 0.16 +1.7 456,945 - Pf1N1B4_388 0.16 -1.7 456,945 - Pf1N1B4_388 0.16 +0.6 456,945 - Pf1N1B4_388 0.16 +0.3 456,945 - Pf1N1B4_388 0.16 -0.1 456,945 - -0.1 456,945 - Pf1N1B4_388 0.16 -1.5 456,945 - Pf1N1B4_388 0.16 +0.1 456,945 - Pf1N1B4_388 0.16 +0.4 457,048 + Pf1N1B4_388 0.29 +0.3 457,049 - Pf1N1B4_388 0.29 -0.3 457,049 - Pf1N1B4_388 0.29 +0.9 457,366 + Pf1N1B4_388 0.68 +1.1 457,367 - Pf1N1B4_388 0.68 -0.9 457,381 + Pf1N1B4_388 0.69 +1.4 457,381 + Pf1N1B4_388 0.69 +1.9 457,381 + Pf1N1B4_388 0.69 -0.1 457,381 + Pf1N1B4_388 0.69 -2.7 457,381 + Pf1N1B4_388 0.69 +1.0 457,382 - Pf1N1B4_388 0.70 +0.9 457,387 + Pf1N1B4_388 0.70 +0.2 457,388 - Pf1N1B4_388 0.70 +0.3 457,388 - Pf1N1B4_388 0.70 +0.1 457,418 - Pf1N1B4_388 0.74 -0.5 457,418 - Pf1N1B4_388 0.74 +0.9 457,571 - +1.4
Or see this region's nucleotide sequence