Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3500

Experiment: L-Leucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3497 and Pf1N1B4_3498 overlap by 1 nucleotidesPf1N1B4_3498 and Pf1N1B4_3499 are separated by 296 nucleotidesPf1N1B4_3499 and Pf1N1B4_3500 are separated by 4 nucleotidesPf1N1B4_3500 and Pf1N1B4_3501 are separated by 199 nucleotides Pf1N1B4_3497 - Heat shock protein, at 3,902,754 to 3,903,158 _3497 Pf1N1B4_3498 - FIG00953240: hypothetical protein, at 3,903,158 to 3,903,592 _3498 Pf1N1B4_3499 - hypothetical protein, at 3,903,889 to 3,904,113 _3499 Pf1N1B4_3500 - D-lactate dehydrogenase (EC 1.1.1.28), at 3,904,118 to 3,905,107 _3500 Pf1N1B4_3501 - BarA sensory histidine kinase (= VarS = GacS), at 3,905,307 to 3,908,057 _3501 Position (kb) 3904 3905 3906Strain fitness (log2 ratio) -2 -1 0 1 2at 3903.452 kb on + strand, within Pf1N1B4_3498at 3903.462 kb on - strandat 3903.462 kb on - strand, within Pf1N1B4_3498at 3903.475 kb on + strand, within Pf1N1B4_3498at 3903.478 kb on + strand, within Pf1N1B4_3498at 3903.478 kb on + strand, within Pf1N1B4_3498at 3903.479 kb on - strand, within Pf1N1B4_3498at 3903.479 kb on - strand, within Pf1N1B4_3498at 3903.571 kb on - strandat 3903.605 kb on + strandat 3903.605 kb on + strandat 3903.605 kb on + strandat 3903.605 kb on + strandat 3903.606 kb on - strandat 3903.606 kb on - strandat 3903.606 kb on - strandat 3903.614 kb on + strandat 3903.654 kb on + strandat 3903.654 kb on + strandat 3903.655 kb on - strandat 3903.655 kb on - strandat 3903.715 kb on + strandat 3903.786 kb on + strandat 3903.787 kb on - strandat 3903.918 kb on + strand, within Pf1N1B4_3499at 3903.919 kb on - strand, within Pf1N1B4_3499at 3904.238 kb on + strand, within Pf1N1B4_3500at 3904.238 kb on + strand, within Pf1N1B4_3500at 3904.238 kb on + strand, within Pf1N1B4_3500at 3904.238 kb on + strand, within Pf1N1B4_3500at 3904.238 kb on + strand, within Pf1N1B4_3500at 3904.238 kb on + strand, within Pf1N1B4_3500at 3904.239 kb on - strand, within Pf1N1B4_3500at 3904.330 kb on - strand, within Pf1N1B4_3500at 3904.330 kb on - strand, within Pf1N1B4_3500at 3904.421 kb on + strand, within Pf1N1B4_3500at 3904.421 kb on + strand, within Pf1N1B4_3500at 3904.421 kb on + strand, within Pf1N1B4_3500at 3904.421 kb on + strand, within Pf1N1B4_3500at 3904.422 kb on - strand, within Pf1N1B4_3500at 3904.737 kb on + strand, within Pf1N1B4_3500at 3904.737 kb on + strand, within Pf1N1B4_3500at 3904.737 kb on + strand, within Pf1N1B4_3500at 3904.737 kb on + strand, within Pf1N1B4_3500at 3904.738 kb on - strandat 3904.809 kb on + strand, within Pf1N1B4_3500at 3904.809 kb on + strand, within Pf1N1B4_3500at 3904.810 kb on - strand, within Pf1N1B4_3500at 3904.929 kb on - strand, within Pf1N1B4_3500at 3905.076 kb on + strandat 3905.077 kb on - strandat 3905.125 kb on + strandat 3905.125 kb on + strandat 3905.126 kb on - strandat 3905.126 kb on - strandat 3905.132 kb on - strandat 3905.219 kb on - strandat 3905.273 kb on + strandat 3905.295 kb on + strandat 3905.607 kb on + strand, within Pf1N1B4_3501at 3905.607 kb on + strandat 3905.608 kb on - strand, within Pf1N1B4_3501at 3905.608 kb on - strand, within Pf1N1B4_3501at 3905.608 kb on - strand, within Pf1N1B4_3501at 3905.752 kb on + strand, within Pf1N1B4_3501at 3905.752 kb on + strand, within Pf1N1B4_3501at 3905.753 kb on - strand, within Pf1N1B4_3501at 3905.753 kb on - strand, within Pf1N1B4_3501at 3905.753 kb on - strand, within Pf1N1B4_3501at 3905.753 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Leucine (C)
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3,903,452 + Pf1N1B4_3498 0.68 -0.7
3,903,462 - +1.8
3,903,462 - Pf1N1B4_3498 0.70 +0.1
3,903,475 + Pf1N1B4_3498 0.73 +0.8
3,903,478 + Pf1N1B4_3498 0.74 +1.8
3,903,478 + Pf1N1B4_3498 0.74 -0.7
3,903,479 - Pf1N1B4_3498 0.74 -1.0
3,903,479 - Pf1N1B4_3498 0.74 -1.0
3,903,571 - -0.2
3,903,605 + +0.3
3,903,605 + +0.3
3,903,605 + +0.8
3,903,605 + -1.2
3,903,606 - +0.4
3,903,606 - -1.7
3,903,606 - +0.3
3,903,614 + -0.4
3,903,654 + -0.9
3,903,654 + -0.7
3,903,655 - -0.2
3,903,655 - -0.4
3,903,715 + +2.2
3,903,786 + -1.2
3,903,787 - -0.2
3,903,918 + Pf1N1B4_3499 0.13 +0.7
3,903,919 - Pf1N1B4_3499 0.13 -0.2
3,904,238 + Pf1N1B4_3500 0.12 -0.2
3,904,238 + Pf1N1B4_3500 0.12 +0.2
3,904,238 + Pf1N1B4_3500 0.12 +1.2
3,904,238 + Pf1N1B4_3500 0.12 +0.8
3,904,238 + Pf1N1B4_3500 0.12 -0.2
3,904,238 + Pf1N1B4_3500 0.12 -2.2
3,904,239 - Pf1N1B4_3500 0.12 +1.8
3,904,330 - Pf1N1B4_3500 0.21 -0.2
3,904,330 - Pf1N1B4_3500 0.21 +0.8
3,904,421 + Pf1N1B4_3500 0.31 -0.2
3,904,421 + Pf1N1B4_3500 0.31 -2.5
3,904,421 + Pf1N1B4_3500 0.31 -1.2
3,904,421 + Pf1N1B4_3500 0.31 -0.8
3,904,422 - Pf1N1B4_3500 0.31 +0.4
3,904,737 + Pf1N1B4_3500 0.63 -0.9
3,904,737 + Pf1N1B4_3500 0.63 -1.2
3,904,737 + Pf1N1B4_3500 0.63 -1.7
3,904,737 + Pf1N1B4_3500 0.63 +0.3
3,904,738 - -0.2
3,904,809 + Pf1N1B4_3500 0.70 +0.0
3,904,809 + Pf1N1B4_3500 0.70 -0.7
3,904,810 - Pf1N1B4_3500 0.70 -0.4
3,904,929 - Pf1N1B4_3500 0.82 +0.4
3,905,076 + +1.1
3,905,077 - -1.7
3,905,125 + +0.3
3,905,125 + -2.5
3,905,126 - -2.2
3,905,126 - +0.1
3,905,132 - +0.4
3,905,219 - +1.8
3,905,273 + +0.8
3,905,295 + -0.3
3,905,607 + Pf1N1B4_3501 0.11 +1.8
3,905,607 + +0.8
3,905,608 - Pf1N1B4_3501 0.11 +1.7
3,905,608 - Pf1N1B4_3501 0.11 +0.4
3,905,608 - Pf1N1B4_3501 0.11 +0.1
3,905,752 + Pf1N1B4_3501 0.16 +0.4
3,905,752 + Pf1N1B4_3501 0.16 +0.4
3,905,753 - Pf1N1B4_3501 0.16 +0.8
3,905,753 - Pf1N1B4_3501 0.16 +0.4
3,905,753 - Pf1N1B4_3501 0.16 +0.4
3,905,753 - +0.3

Or see this region's nucleotide sequence