Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3497 and Pf1N1B4_3498 overlap by 1 nucleotides Pf1N1B4_3498 and Pf1N1B4_3499 are separated by 296 nucleotides Pf1N1B4_3499 and Pf1N1B4_3500 are separated by 4 nucleotides Pf1N1B4_3500 and Pf1N1B4_3501 are separated by 199 nucleotides
Pf1N1B4_3497 - Heat shock protein, at 3,902,754 to 3,903,158
_3497
Pf1N1B4_3498 - FIG00953240: hypothetical protein, at 3,903,158 to 3,903,592
_3498
Pf1N1B4_3499 - hypothetical protein, at 3,903,889 to 3,904,113
_3499
Pf1N1B4_3500 - D-lactate dehydrogenase (EC 1.1.1.28), at 3,904,118 to 3,905,107
_3500
Pf1N1B4_3501 - BarA sensory histidine kinase (= VarS = GacS), at 3,905,307 to 3,908,057
_3501
Position (kb)
3904
3905
3906 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3903.452 kb on + strand, within Pf1N1B4_3498 at 3903.462 kb on - strand at 3903.462 kb on - strand, within Pf1N1B4_3498 at 3903.475 kb on + strand, within Pf1N1B4_3498 at 3903.478 kb on + strand, within Pf1N1B4_3498 at 3903.478 kb on + strand, within Pf1N1B4_3498 at 3903.479 kb on - strand, within Pf1N1B4_3498 at 3903.479 kb on - strand, within Pf1N1B4_3498 at 3903.571 kb on - strand at 3903.605 kb on + strand at 3903.605 kb on + strand at 3903.605 kb on + strand at 3903.605 kb on + strand at 3903.606 kb on - strand at 3903.606 kb on - strand at 3903.606 kb on - strand at 3903.614 kb on + strand at 3903.654 kb on + strand at 3903.654 kb on + strand at 3903.655 kb on - strand at 3903.655 kb on - strand at 3903.715 kb on + strand at 3903.786 kb on + strand at 3903.787 kb on - strand at 3903.918 kb on + strand, within Pf1N1B4_3499 at 3903.919 kb on - strand, within Pf1N1B4_3499 at 3904.238 kb on + strand, within Pf1N1B4_3500 at 3904.238 kb on + strand, within Pf1N1B4_3500 at 3904.238 kb on + strand, within Pf1N1B4_3500 at 3904.238 kb on + strand, within Pf1N1B4_3500 at 3904.238 kb on + strand, within Pf1N1B4_3500 at 3904.238 kb on + strand, within Pf1N1B4_3500 at 3904.239 kb on - strand, within Pf1N1B4_3500 at 3904.330 kb on - strand, within Pf1N1B4_3500 at 3904.330 kb on - strand, within Pf1N1B4_3500 at 3904.421 kb on + strand, within Pf1N1B4_3500 at 3904.421 kb on + strand, within Pf1N1B4_3500 at 3904.421 kb on + strand, within Pf1N1B4_3500 at 3904.421 kb on + strand, within Pf1N1B4_3500 at 3904.422 kb on - strand, within Pf1N1B4_3500 at 3904.737 kb on + strand, within Pf1N1B4_3500 at 3904.737 kb on + strand, within Pf1N1B4_3500 at 3904.737 kb on + strand, within Pf1N1B4_3500 at 3904.737 kb on + strand, within Pf1N1B4_3500 at 3904.738 kb on - strand at 3904.809 kb on + strand, within Pf1N1B4_3500 at 3904.809 kb on + strand, within Pf1N1B4_3500 at 3904.810 kb on - strand, within Pf1N1B4_3500 at 3904.929 kb on - strand, within Pf1N1B4_3500 at 3905.076 kb on + strand at 3905.077 kb on - strand at 3905.125 kb on + strand at 3905.125 kb on + strand at 3905.126 kb on - strand at 3905.126 kb on - strand at 3905.132 kb on - strand at 3905.219 kb on - strand at 3905.273 kb on + strand at 3905.295 kb on + strand at 3905.607 kb on + strand, within Pf1N1B4_3501 at 3905.607 kb on + strand at 3905.608 kb on - strand, within Pf1N1B4_3501 at 3905.608 kb on - strand, within Pf1N1B4_3501 at 3905.608 kb on - strand, within Pf1N1B4_3501 at 3905.752 kb on + strand, within Pf1N1B4_3501 at 3905.752 kb on + strand, within Pf1N1B4_3501 at 3905.753 kb on - strand, within Pf1N1B4_3501 at 3905.753 kb on - strand, within Pf1N1B4_3501 at 3905.753 kb on - strand, within Pf1N1B4_3501 at 3905.753 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 3,903,452 + Pf1N1B4_3498 0.68 -0.7 3,903,462 - +1.8 3,903,462 - Pf1N1B4_3498 0.70 +0.1 3,903,475 + Pf1N1B4_3498 0.73 +0.8 3,903,478 + Pf1N1B4_3498 0.74 +1.8 3,903,478 + Pf1N1B4_3498 0.74 -0.7 3,903,479 - Pf1N1B4_3498 0.74 -1.0 3,903,479 - Pf1N1B4_3498 0.74 -1.0 3,903,571 - -0.2 3,903,605 + +0.3 3,903,605 + +0.3 3,903,605 + +0.8 3,903,605 + -1.2 3,903,606 - +0.4 3,903,606 - -1.7 3,903,606 - +0.3 3,903,614 + -0.4 3,903,654 + -0.9 3,903,654 + -0.7 3,903,655 - -0.2 3,903,655 - -0.4 3,903,715 + +2.2 3,903,786 + -1.2 3,903,787 - -0.2 3,903,918 + Pf1N1B4_3499 0.13 +0.7 3,903,919 - Pf1N1B4_3499 0.13 -0.2 3,904,238 + Pf1N1B4_3500 0.12 -0.2 3,904,238 + Pf1N1B4_3500 0.12 +0.2 3,904,238 + Pf1N1B4_3500 0.12 +1.2 3,904,238 + Pf1N1B4_3500 0.12 +0.8 3,904,238 + Pf1N1B4_3500 0.12 -0.2 3,904,238 + Pf1N1B4_3500 0.12 -2.2 3,904,239 - Pf1N1B4_3500 0.12 +1.8 3,904,330 - Pf1N1B4_3500 0.21 -0.2 3,904,330 - Pf1N1B4_3500 0.21 +0.8 3,904,421 + Pf1N1B4_3500 0.31 -0.2 3,904,421 + Pf1N1B4_3500 0.31 -2.5 3,904,421 + Pf1N1B4_3500 0.31 -1.2 3,904,421 + Pf1N1B4_3500 0.31 -0.8 3,904,422 - Pf1N1B4_3500 0.31 +0.4 3,904,737 + Pf1N1B4_3500 0.63 -0.9 3,904,737 + Pf1N1B4_3500 0.63 -1.2 3,904,737 + Pf1N1B4_3500 0.63 -1.7 3,904,737 + Pf1N1B4_3500 0.63 +0.3 3,904,738 - -0.2 3,904,809 + Pf1N1B4_3500 0.70 +0.0 3,904,809 + Pf1N1B4_3500 0.70 -0.7 3,904,810 - Pf1N1B4_3500 0.70 -0.4 3,904,929 - Pf1N1B4_3500 0.82 +0.4 3,905,076 + +1.1 3,905,077 - -1.7 3,905,125 + +0.3 3,905,125 + -2.5 3,905,126 - -2.2 3,905,126 - +0.1 3,905,132 - +0.4 3,905,219 - +1.8 3,905,273 + +0.8 3,905,295 + -0.3 3,905,607 + Pf1N1B4_3501 0.11 +1.8 3,905,607 + +0.8 3,905,608 - Pf1N1B4_3501 0.11 +1.7 3,905,608 - Pf1N1B4_3501 0.11 +0.4 3,905,608 - Pf1N1B4_3501 0.11 +0.1 3,905,752 + Pf1N1B4_3501 0.16 +0.4 3,905,752 + Pf1N1B4_3501 0.16 +0.4 3,905,753 - Pf1N1B4_3501 0.16 +0.8 3,905,753 - Pf1N1B4_3501 0.16 +0.4 3,905,753 - Pf1N1B4_3501 0.16 +0.4 3,905,753 - +0.3
Or see this region's nucleotide sequence