Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_270 and Pf1N1B4_271 are separated by 64 nucleotides Pf1N1B4_271 and Pf1N1B4_272 are separated by 96 nucleotides Pf1N1B4_272 and Pf1N1B4_273 overlap by 11 nucleotides Pf1N1B4_273 and Pf1N1B4_274 are separated by 72 nucleotides
Pf1N1B4_270 - FIG074102: hypothetical protein, at 330,359 to 331,588
_270
Pf1N1B4_271 - FIG00953957: hypothetical protein, at 331,653 to 331,868
_271
Pf1N1B4_272 - Transcriptional regulator, LysR family, at 331,965 to 332,819
_272
Pf1N1B4_273 - putative membrane protein, at 332,809 to 333,558
_273
Pf1N1B4_274 - Transcriptional regulator, LysR family, at 333,631 to 334,623
_274
Position (kb) 331
332
333 Strain fitness (log2 ratio) -2
-1
0
1
2 at 331.230 kb on + strand at 331.230 kb on + strand, within Pf1N1B4_270 at 331.230 kb on + strand, within Pf1N1B4_270 at 331.230 kb on + strand, within Pf1N1B4_270 at 331.231 kb on - strand, within Pf1N1B4_270 at 331.231 kb on - strand, within Pf1N1B4_270 at 331.231 kb on - strand, within Pf1N1B4_270 at 331.231 kb on - strand, within Pf1N1B4_270 at 331.231 kb on - strand, within Pf1N1B4_270 at 331.231 kb on - strand, within Pf1N1B4_270 at 331.231 kb on - strand, within Pf1N1B4_270 at 331.281 kb on + strand, within Pf1N1B4_270 at 331.282 kb on - strand, within Pf1N1B4_270 at 331.383 kb on + strand, within Pf1N1B4_270 at 331.383 kb on + strand, within Pf1N1B4_270 at 331.383 kb on + strand at 331.383 kb on + strand, within Pf1N1B4_270 at 331.384 kb on - strand, within Pf1N1B4_270 at 331.414 kb on - strand, within Pf1N1B4_270 at 331.615 kb on + strand at 331.616 kb on - strand at 331.659 kb on - strand at 331.659 kb on - strand at 331.659 kb on - strand at 331.846 kb on - strand, within Pf1N1B4_271 at 331.871 kb on + strand at 331.872 kb on - strand at 331.945 kb on + strand at 331.946 kb on - strand at 331.946 kb on - strand at 331.946 kb on - strand at 331.946 kb on - strand at 331.946 kb on - strand at 331.946 kb on - strand at 331.946 kb on - strand at 331.946 kb on - strand at 331.946 kb on - strand at 331.996 kb on + strand at 331.997 kb on - strand at 331.997 kb on - strand at 331.997 kb on - strand at 331.997 kb on - strand at 331.997 kb on - strand at 331.997 kb on - strand at 331.997 kb on - strand at 332.036 kb on + strand at 332.037 kb on - strand at 332.173 kb on + strand, within Pf1N1B4_272 at 332.174 kb on - strand, within Pf1N1B4_272 at 332.174 kb on - strand, within Pf1N1B4_272 at 332.174 kb on - strand, within Pf1N1B4_272 at 332.174 kb on - strand, within Pf1N1B4_272 at 332.185 kb on - strand, within Pf1N1B4_272 at 332.226 kb on - strand, within Pf1N1B4_272 at 332.226 kb on - strand, within Pf1N1B4_272 at 332.281 kb on - strand at 332.595 kb on + strand at 332.595 kb on + strand, within Pf1N1B4_272 at 332.595 kb on + strand, within Pf1N1B4_272 at 332.596 kb on - strand, within Pf1N1B4_272 at 332.596 kb on - strand, within Pf1N1B4_272 at 332.596 kb on - strand, within Pf1N1B4_272 at 332.818 kb on - strand at 332.981 kb on + strand, within Pf1N1B4_273 at 332.981 kb on + strand at 332.982 kb on - strand, within Pf1N1B4_273 at 332.982 kb on - strand, within Pf1N1B4_273 at 332.982 kb on - strand at 332.982 kb on - strand, within Pf1N1B4_273 at 332.982 kb on - strand, within Pf1N1B4_273 at 332.982 kb on - strand, within Pf1N1B4_273 at 333.003 kb on + strand, within Pf1N1B4_273 at 333.004 kb on - strand, within Pf1N1B4_273 at 333.165 kb on - strand, within Pf1N1B4_273 at 333.219 kb on - strand, within Pf1N1B4_273 at 333.539 kb on + strand at 333.539 kb on + strand at 333.539 kb on + strand at 333.539 kb on + strand at 333.540 kb on - strand at 333.540 kb on - strand at 333.540 kb on - strand at 333.590 kb on + strand at 333.591 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 331,230 + -1.2 331,230 + Pf1N1B4_270 0.71 -0.2 331,230 + Pf1N1B4_270 0.71 -1.2 331,230 + Pf1N1B4_270 0.71 -1.2 331,231 - Pf1N1B4_270 0.71 -0.2 331,231 - Pf1N1B4_270 0.71 +0.8 331,231 - Pf1N1B4_270 0.71 +0.3 331,231 - Pf1N1B4_270 0.71 +0.1 331,231 - Pf1N1B4_270 0.71 +0.8 331,231 - Pf1N1B4_270 0.71 -0.8 331,231 - Pf1N1B4_270 0.71 -0.2 331,281 + Pf1N1B4_270 0.75 +1.6 331,282 - Pf1N1B4_270 0.75 +1.1 331,383 + Pf1N1B4_270 0.83 -2.2 331,383 + Pf1N1B4_270 0.83 -0.4 331,383 + +0.8 331,383 + Pf1N1B4_270 0.83 +1.8 331,384 - Pf1N1B4_270 0.83 +1.5 331,414 - Pf1N1B4_270 0.86 -1.8 331,615 + -1.2 331,616 - +0.2 331,659 - -0.2 331,659 - +1.8 331,659 - -2.2 331,846 - Pf1N1B4_271 0.89 +0.9 331,871 + -1.2 331,872 - -2.5 331,945 + +0.2 331,946 - +0.4 331,946 - -2.2 331,946 - +1.0 331,946 - +1.4 331,946 - +0.3 331,946 - +1.6 331,946 - -1.8 331,946 - +0.5 331,946 - -0.9 331,996 + -0.3 331,997 - +0.8 331,997 - -0.6 331,997 - +0.3 331,997 - -0.2 331,997 - +0.3 331,997 - -0.2 331,997 - -0.8 332,036 + -0.2 332,037 - -1.2 332,173 + Pf1N1B4_272 0.24 +1.9 332,174 - Pf1N1B4_272 0.24 +0.2 332,174 - Pf1N1B4_272 0.24 -0.3 332,174 - Pf1N1B4_272 0.24 +0.4 332,174 - Pf1N1B4_272 0.24 -0.2 332,185 - Pf1N1B4_272 0.26 +0.2 332,226 - Pf1N1B4_272 0.31 +1.8 332,226 - Pf1N1B4_272 0.31 +0.8 332,281 - +1.4 332,595 + +1.4 332,595 + Pf1N1B4_272 0.74 +0.8 332,595 + Pf1N1B4_272 0.74 +0.2 332,596 - Pf1N1B4_272 0.74 +0.6 332,596 - Pf1N1B4_272 0.74 +0.8 332,596 - Pf1N1B4_272 0.74 -0.5 332,818 - -0.2 332,981 + Pf1N1B4_273 0.23 -0.2 332,981 + -1.2 332,982 - Pf1N1B4_273 0.23 +0.1 332,982 - Pf1N1B4_273 0.23 +0.8 332,982 - +2.4 332,982 - Pf1N1B4_273 0.23 -1.4 332,982 - Pf1N1B4_273 0.23 -0.2 332,982 - Pf1N1B4_273 0.23 -0.3 333,003 + Pf1N1B4_273 0.26 -0.2 333,004 - Pf1N1B4_273 0.26 -1.2 333,165 - Pf1N1B4_273 0.47 -0.2 333,219 - Pf1N1B4_273 0.55 +0.6 333,539 + -1.2 333,539 + +0.6 333,539 + -0.2 333,539 + -1.2 333,540 - +0.4 333,540 - -0.8 333,540 - +0.8 333,590 + +1.8 333,591 - +0.8
Or see this region's nucleotide sequence