Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2709 and Pf1N1B4_2710 are separated by 5 nucleotides Pf1N1B4_2710 and Pf1N1B4_2711 overlap by 8 nucleotides Pf1N1B4_2711 and Pf1N1B4_2712 are separated by 214 nucleotides
Pf1N1B4_2709 - Magnesium and cobalt efflux protein CorC, at 3,047,156 to 3,047,995
_2709
Pf1N1B4_2710 - Metal-dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly, at 3,048,001 to 3,048,495
_2710
Pf1N1B4_2711 - Phosphate starvation-inducible protein PhoH, predicted ATPase, at 3,048,488 to 3,049,504
_2711
Pf1N1B4_2712 - tRNA-i(6)A37 methylthiotransferase, at 3,049,719 to 3,051,095
_2712
Position (kb)
3048
3049
3050 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3047.807 kb on + strand, within Pf1N1B4_2709 at 3048.023 kb on - strand at 3048.838 kb on - strand, within Pf1N1B4_2711 at 3048.838 kb on - strand, within Pf1N1B4_2711 at 3048.889 kb on - strand, within Pf1N1B4_2711 at 3049.072 kb on - strand, within Pf1N1B4_2711 at 3049.072 kb on - strand, within Pf1N1B4_2711 at 3049.072 kb on - strand, within Pf1N1B4_2711 at 3049.138 kb on - strand at 3049.533 kb on - strand at 3049.579 kb on - strand at 3049.580 kb on + strand at 3049.660 kb on + strand at 3049.660 kb on + strand at 3050.048 kb on - strand, within Pf1N1B4_2712 at 3050.048 kb on - strand, within Pf1N1B4_2712 at 3050.192 kb on - strand, within Pf1N1B4_2712
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 3,047,807 + Pf1N1B4_2709 0.78 +0.2 3,048,023 - +0.8 3,048,838 - Pf1N1B4_2711 0.34 -0.2 3,048,838 - Pf1N1B4_2711 0.34 +1.8 3,048,889 - Pf1N1B4_2711 0.39 -0.8 3,049,072 - Pf1N1B4_2711 0.57 -2.4 3,049,072 - Pf1N1B4_2711 0.57 -1.4 3,049,072 - Pf1N1B4_2711 0.57 -1.4 3,049,138 - -1.4 3,049,533 - +0.2 3,049,579 - -1.4 3,049,580 + -0.8 3,049,660 + +2.2 3,049,660 + -0.2 3,050,048 - Pf1N1B4_2712 0.24 +1.8 3,050,048 - Pf1N1B4_2712 0.24 +0.2 3,050,192 - Pf1N1B4_2712 0.34 -0.4
Or see this region's nucleotide sequence