Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_175 and Pf1N1B4_176 are separated by 247 nucleotides Pf1N1B4_176 and Pf1N1B4_177 are separated by 151 nucleotides
Pf1N1B4_175 - sigma-54 dependent transcriptional regulator, at 217,172 to 219,076
_175
Pf1N1B4_176 - Acetylpolyamine aminohydrolase, at 219,324 to 220,349
_176
Pf1N1B4_177 - N-carbamoyl-L-amino acid hydrolase (EC 3.5.1.87), at 220,501 to 221,730
_177
Position (kb) 219
220
221 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 218.388 kb on + strand, within Pf1N1B4_175 at 218.563 kb on - strand at 218.608 kb on - strand, within Pf1N1B4_175 at 218.608 kb on - strand, within Pf1N1B4_175 at 218.981 kb on + strand at 218.981 kb on + strand at 219.044 kb on + strand at 219.044 kb on + strand at 219.045 kb on - strand at 219.045 kb on - strand at 219.045 kb on - strand at 219.069 kb on - strand at 219.169 kb on - strand at 219.169 kb on - strand at 219.306 kb on + strand at 219.307 kb on - strand at 219.507 kb on - strand, within Pf1N1B4_176 at 219.718 kb on + strand, within Pf1N1B4_176 at 219.719 kb on - strand, within Pf1N1B4_176 at 219.719 kb on - strand, within Pf1N1B4_176 at 219.719 kb on - strand, within Pf1N1B4_176 at 219.847 kb on + strand, within Pf1N1B4_176 at 219.847 kb on + strand, within Pf1N1B4_176 at 219.848 kb on - strand, within Pf1N1B4_176 at 219.908 kb on - strand, within Pf1N1B4_176 at 220.021 kb on + strand, within Pf1N1B4_176 at 220.022 kb on - strand, within Pf1N1B4_176 at 220.153 kb on + strand, within Pf1N1B4_176 at 220.277 kb on + strand at 220.298 kb on - strand at 220.298 kb on - strand at 220.298 kb on - strand at 220.300 kb on + strand at 220.300 kb on + strand at 220.300 kb on + strand at 220.300 kb on + strand at 220.300 kb on + strand at 220.300 kb on + strand at 220.301 kb on - strand at 220.301 kb on - strand at 220.301 kb on - strand at 220.358 kb on + strand at 220.358 kb on + strand at 220.374 kb on + strand at 220.374 kb on + strand at 220.374 kb on + strand at 220.374 kb on + strand at 220.374 kb on + strand at 220.375 kb on - strand at 220.503 kb on - strand at 220.782 kb on + strand, within Pf1N1B4_177 at 220.783 kb on - strand, within Pf1N1B4_177 at 220.783 kb on - strand, within Pf1N1B4_177 at 220.929 kb on + strand, within Pf1N1B4_177 at 220.930 kb on - strand, within Pf1N1B4_177 at 220.930 kb on - strand, within Pf1N1B4_177 at 220.930 kb on - strand, within Pf1N1B4_177 at 220.930 kb on - strand, within Pf1N1B4_177 at 221.053 kb on - strand at 221.053 kb on - strand, within Pf1N1B4_177 at 221.053 kb on - strand, within Pf1N1B4_177 at 221.183 kb on + strand, within Pf1N1B4_177 at 221.184 kb on - strand at 221.184 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 218,388 + Pf1N1B4_175 0.64 -1.6 218,563 - +0.3 218,608 - Pf1N1B4_175 0.75 -3.1 218,608 - Pf1N1B4_175 0.75 -0.2 218,981 + +0.3 218,981 + -0.6 219,044 + -0.2 219,044 + -0.2 219,045 - -0.2 219,045 - -1.2 219,045 - -0.6 219,069 - +1.8 219,169 - -0.7 219,169 - +0.6 219,306 + -0.7 219,307 - -1.0 219,507 - Pf1N1B4_176 0.18 -1.6 219,718 + Pf1N1B4_176 0.38 -1.5 219,719 - Pf1N1B4_176 0.38 -2.2 219,719 - Pf1N1B4_176 0.38 -0.8 219,719 - Pf1N1B4_176 0.38 +0.3 219,847 + Pf1N1B4_176 0.51 -1.5 219,847 + Pf1N1B4_176 0.51 +1.3 219,848 - Pf1N1B4_176 0.51 -0.2 219,908 - Pf1N1B4_176 0.57 -1.5 220,021 + Pf1N1B4_176 0.68 +2.1 220,022 - Pf1N1B4_176 0.68 -1.2 220,153 + Pf1N1B4_176 0.81 -0.2 220,277 + -1.2 220,298 - +0.4 220,298 - -1.7 220,298 - -0.8 220,300 + -0.2 220,300 + -0.2 220,300 + -0.2 220,300 + -1.0 220,300 + +1.1 220,300 + -1.2 220,301 - +0.3 220,301 - +1.8 220,301 - -0.7 220,358 + +0.1 220,358 + -1.0 220,374 + -1.0 220,374 + +0.0 220,374 + -0.2 220,374 + +0.0 220,374 + -0.2 220,375 - +2.3 220,503 - +1.3 220,782 + Pf1N1B4_177 0.23 +0.3 220,783 - Pf1N1B4_177 0.23 +0.4 220,783 - Pf1N1B4_177 0.23 -0.8 220,929 + Pf1N1B4_177 0.35 -1.5 220,930 - Pf1N1B4_177 0.35 -0.2 220,930 - Pf1N1B4_177 0.35 +0.3 220,930 - Pf1N1B4_177 0.35 -0.2 220,930 - Pf1N1B4_177 0.35 -0.6 221,053 - -1.2 221,053 - Pf1N1B4_177 0.45 +0.8 221,053 - Pf1N1B4_177 0.45 +0.8 221,183 + Pf1N1B4_177 0.55 +0.0 221,184 - +0.8 221,184 - -0.2
Or see this region's nucleotide sequence