Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_170 and Pf1N1B4_171 are separated by 332 nucleotides Pf1N1B4_171 and Pf1N1B4_172 are separated by 139 nucleotides
Pf1N1B4_170 - FIG00962118: hypothetical protein, at 211,335 to 211,589
_170
Pf1N1B4_171 - Aldehyde dehydrogenase (EC 1.2.1.3), at 211,922 to 213,355
_171
Pf1N1B4_172 - Probable alkylhalidase homolog (EC 3.8.1.1), at 213,495 to 214,790
_172
Position (kb) 211
212
213
214 Strain fitness (log2 ratio) -2
-1
0
1
2 at 210.938 kb on + strand at 210.939 kb on - strand at 210.960 kb on + strand at 210.961 kb on - strand at 211.024 kb on - strand at 211.058 kb on - strand at 211.155 kb on + strand at 211.155 kb on + strand at 211.155 kb on + strand at 211.155 kb on + strand at 211.155 kb on + strand at 211.155 kb on + strand at 211.155 kb on + strand at 211.155 kb on + strand at 211.155 kb on + strand at 211.156 kb on - strand at 211.156 kb on - strand at 211.156 kb on - strand at 211.156 kb on - strand at 211.156 kb on - strand at 211.156 kb on - strand at 211.343 kb on - strand at 211.593 kb on + strand at 211.594 kb on - strand at 211.639 kb on - strand at 211.904 kb on - strand at 212.189 kb on - strand, within Pf1N1B4_171 at 212.220 kb on + strand, within Pf1N1B4_171 at 212.220 kb on + strand, within Pf1N1B4_171 at 212.220 kb on + strand at 212.220 kb on + strand, within Pf1N1B4_171 at 212.220 kb on + strand, within Pf1N1B4_171 at 212.221 kb on - strand, within Pf1N1B4_171 at 212.221 kb on - strand, within Pf1N1B4_171 at 212.221 kb on - strand at 212.221 kb on - strand, within Pf1N1B4_171 at 212.221 kb on - strand, within Pf1N1B4_171 at 212.221 kb on - strand, within Pf1N1B4_171 at 212.246 kb on + strand, within Pf1N1B4_171 at 212.246 kb on + strand, within Pf1N1B4_171 at 212.247 kb on - strand, within Pf1N1B4_171 at 212.446 kb on + strand, within Pf1N1B4_171 at 212.641 kb on - strand, within Pf1N1B4_171 at 212.795 kb on + strand, within Pf1N1B4_171 at 212.795 kb on + strand, within Pf1N1B4_171 at 212.796 kb on - strand, within Pf1N1B4_171 at 212.796 kb on - strand, within Pf1N1B4_171 at 212.796 kb on - strand, within Pf1N1B4_171 at 212.796 kb on - strand, within Pf1N1B4_171 at 213.320 kb on + strand at 213.353 kb on + strand at 213.353 kb on + strand at 213.354 kb on - strand at 213.504 kb on - strand at 213.504 kb on - strand at 213.504 kb on - strand at 213.504 kb on - strand at 213.504 kb on - strand at 213.506 kb on - strand at 213.506 kb on - strand at 213.506 kb on - strand at 213.699 kb on + strand, within Pf1N1B4_172 at 213.820 kb on + strand, within Pf1N1B4_172 at 213.821 kb on - strand, within Pf1N1B4_172 at 213.821 kb on - strand, within Pf1N1B4_172 at 213.821 kb on - strand, within Pf1N1B4_172 at 213.918 kb on + strand, within Pf1N1B4_172 at 213.919 kb on - strand, within Pf1N1B4_172 at 213.919 kb on - strand at 214.083 kb on + strand, within Pf1N1B4_172 at 214.083 kb on + strand, within Pf1N1B4_172 at 214.084 kb on - strand, within Pf1N1B4_172
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 210,938 + -0.8 210,939 - -0.2 210,960 + -0.8 210,961 - +0.8 211,024 - +0.2 211,058 - +0.1 211,155 + +1.8 211,155 + +0.3 211,155 + -0.8 211,155 + -0.2 211,155 + -0.4 211,155 + +1.1 211,155 + +0.8 211,155 + +0.8 211,155 + +0.8 211,156 - -0.2 211,156 - +0.3 211,156 - +0.2 211,156 - +0.8 211,156 - -0.8 211,156 - -1.8 211,343 - -0.8 211,593 + -0.7 211,594 - -0.2 211,639 - -1.2 211,904 - +0.2 212,189 - Pf1N1B4_171 0.19 -0.2 212,220 + Pf1N1B4_171 0.21 -1.0 212,220 + Pf1N1B4_171 0.21 +0.3 212,220 + -0.2 212,220 + Pf1N1B4_171 0.21 -0.2 212,220 + Pf1N1B4_171 0.21 +1.1 212,221 - Pf1N1B4_171 0.21 +0.8 212,221 - Pf1N1B4_171 0.21 -0.2 212,221 - +0.8 212,221 - Pf1N1B4_171 0.21 -2.6 212,221 - Pf1N1B4_171 0.21 +1.0 212,221 - Pf1N1B4_171 0.21 -1.1 212,246 + Pf1N1B4_171 0.23 -1.2 212,246 + Pf1N1B4_171 0.23 -0.4 212,247 - Pf1N1B4_171 0.23 -1.8 212,446 + Pf1N1B4_171 0.37 +0.2 212,641 - Pf1N1B4_171 0.50 -1.8 212,795 + Pf1N1B4_171 0.61 +0.6 212,795 + Pf1N1B4_171 0.61 +1.1 212,796 - Pf1N1B4_171 0.61 +1.3 212,796 - Pf1N1B4_171 0.61 +0.2 212,796 - Pf1N1B4_171 0.61 +0.3 212,796 - Pf1N1B4_171 0.61 -1.6 213,320 + -1.5 213,353 + +0.8 213,353 + -0.2 213,354 - +0.8 213,504 - +0.8 213,504 - +0.6 213,504 - -0.2 213,504 - +0.0 213,504 - +0.2 213,506 - +1.3 213,506 - -1.6 213,506 - -0.1 213,699 + Pf1N1B4_172 0.16 +0.3 213,820 + Pf1N1B4_172 0.25 -2.2 213,821 - Pf1N1B4_172 0.25 +0.3 213,821 - Pf1N1B4_172 0.25 -0.5 213,821 - Pf1N1B4_172 0.25 +1.1 213,918 + Pf1N1B4_172 0.33 -0.2 213,919 - Pf1N1B4_172 0.33 -0.1 213,919 - +0.8 214,083 + Pf1N1B4_172 0.45 -0.2 214,083 + Pf1N1B4_172 0.45 -1.8 214,084 - Pf1N1B4_172 0.45 +0.3
Or see this region's nucleotide sequence