Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_10 and Pf1N1B4_11 are separated by 280 nucleotides Pf1N1B4_11 and Pf1N1B4_12 are separated by 67 nucleotides
Pf1N1B4_10 - Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11), at 10,802 to 11,812
_10
Pf1N1B4_11 - Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11), at 12,093 to 13,205
_11
Pf1N1B4_12 - 3-isopropylmalate dehydrogenase (EC 1.1.1.85), at 13,273 to 14,355
_12
Position (kb) 12
13
14 Strain fitness (log2 ratio) -2
-1
0
1
2 at 11.107 kb on + strand, within Pf1N1B4_10 at 11.108 kb on - strand, within Pf1N1B4_10 at 11.108 kb on - strand, within Pf1N1B4_10 at 11.185 kb on + strand, within Pf1N1B4_10 at 11.186 kb on - strand, within Pf1N1B4_10 at 11.794 kb on + strand at 11.818 kb on + strand at 11.818 kb on + strand at 11.819 kb on - strand at 11.819 kb on - strand at 11.901 kb on - strand at 11.952 kb on + strand at 11.953 kb on - strand at 12.006 kb on + strand at 12.081 kb on + strand at 12.081 kb on + strand at 12.081 kb on + strand at 12.082 kb on - strand at 12.082 kb on - strand at 13.223 kb on - strand at 13.223 kb on - strand at 13.298 kb on - strand at 13.378 kb on + strand at 13.526 kb on + strand, within Pf1N1B4_12 at 13.526 kb on + strand at 13.526 kb on + strand, within Pf1N1B4_12 at 13.526 kb on + strand, within Pf1N1B4_12 at 13.526 kb on + strand, within Pf1N1B4_12 at 13.526 kb on + strand, within Pf1N1B4_12 at 13.526 kb on + strand, within Pf1N1B4_12 at 13.526 kb on + strand at 13.527 kb on - strand, within Pf1N1B4_12 at 13.527 kb on - strand, within Pf1N1B4_12 at 13.691 kb on + strand, within Pf1N1B4_12 at 13.691 kb on + strand, within Pf1N1B4_12 at 13.691 kb on + strand, within Pf1N1B4_12 at 13.691 kb on + strand, within Pf1N1B4_12 at 13.691 kb on + strand at 13.691 kb on + strand, within Pf1N1B4_12 at 13.691 kb on + strand, within Pf1N1B4_12 at 13.691 kb on + strand, within Pf1N1B4_12 at 13.691 kb on + strand, within Pf1N1B4_12 at 13.691 kb on + strand, within Pf1N1B4_12 at 13.692 kb on - strand, within Pf1N1B4_12 at 13.692 kb on - strand, within Pf1N1B4_12 at 13.692 kb on - strand, within Pf1N1B4_12 at 13.692 kb on - strand, within Pf1N1B4_12 at 13.692 kb on - strand, within Pf1N1B4_12 at 13.881 kb on - strand, within Pf1N1B4_12 at 13.881 kb on - strand, within Pf1N1B4_12 at 13.881 kb on - strand, within Pf1N1B4_12 at 13.934 kb on + strand, within Pf1N1B4_12 at 13.934 kb on + strand, within Pf1N1B4_12 at 13.934 kb on + strand, within Pf1N1B4_12 at 13.935 kb on - strand, within Pf1N1B4_12 at 14.150 kb on - strand, within Pf1N1B4_12
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 11,107 + Pf1N1B4_10 0.30 -0.6 11,108 - Pf1N1B4_10 0.30 -0.8 11,108 - Pf1N1B4_10 0.30 -1.2 11,185 + Pf1N1B4_10 0.38 +1.5 11,186 - Pf1N1B4_10 0.38 -1.8 11,794 + -2.2 11,818 + +0.0 11,818 + +0.0 11,819 - +0.8 11,819 - -0.2 11,901 - -0.7 11,952 + -0.8 11,953 - -0.2 12,006 + +1.3 12,081 + -0.4 12,081 + +0.3 12,081 + -1.2 12,082 - -2.1 12,082 - -0.2 13,223 - +0.0 13,223 - -0.2 13,298 - +0.6 13,378 + +1.3 13,526 + Pf1N1B4_12 0.23 +0.8 13,526 + -1.8 13,526 + Pf1N1B4_12 0.23 +0.3 13,526 + Pf1N1B4_12 0.23 +1.0 13,526 + Pf1N1B4_12 0.23 -0.2 13,526 + Pf1N1B4_12 0.23 -0.5 13,526 + Pf1N1B4_12 0.23 +0.2 13,526 + +0.3 13,527 - Pf1N1B4_12 0.23 +0.3 13,527 - Pf1N1B4_12 0.23 -0.2 13,691 + Pf1N1B4_12 0.39 -1.0 13,691 + Pf1N1B4_12 0.39 -1.8 13,691 + Pf1N1B4_12 0.39 -1.4 13,691 + Pf1N1B4_12 0.39 -0.5 13,691 + -0.2 13,691 + Pf1N1B4_12 0.39 -0.2 13,691 + Pf1N1B4_12 0.39 -1.2 13,691 + Pf1N1B4_12 0.39 -1.2 13,691 + Pf1N1B4_12 0.39 -0.8 13,691 + Pf1N1B4_12 0.39 -0.1 13,692 - Pf1N1B4_12 0.39 +1.3 13,692 - Pf1N1B4_12 0.39 +0.2 13,692 - Pf1N1B4_12 0.39 -0.8 13,692 - Pf1N1B4_12 0.39 +1.8 13,692 - Pf1N1B4_12 0.39 -1.0 13,881 - Pf1N1B4_12 0.56 +0.6 13,881 - Pf1N1B4_12 0.56 +0.8 13,881 - Pf1N1B4_12 0.56 -0.7 13,934 + Pf1N1B4_12 0.61 -1.8 13,934 + Pf1N1B4_12 0.61 -1.7 13,934 + Pf1N1B4_12 0.61 +0.8 13,935 - Pf1N1B4_12 0.61 -2.6 14,150 - Pf1N1B4_12 0.81 -0.8
Or see this region's nucleotide sequence