Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1086 and Pf1N1B4_1087 are separated by 1 nucleotides Pf1N1B4_1087 and Pf1N1B4_1088 are separated by 27 nucleotides Pf1N1B4_1088 and Pf1N1B4_1089 overlap by 8 nucleotides Pf1N1B4_1089 and Pf1N1B4_1090 are separated by 247 nucleotides
Pf1N1B4_1086 - Manganese superoxide dismutase (EC 1.15.1.1), at 1,216,684 to 1,217,295
_1086
Pf1N1B4_1087 - FOG: TPR repeat, at 1,217,297 to 1,217,737
_1087
Pf1N1B4_1088 - Fumarate hydratase class II (EC 4.2.1.2), at 1,217,765 to 1,219,141
_1088
Pf1N1B4_1089 - FagA protein, at 1,219,134 to 1,219,529
_1089
Pf1N1B4_1090 - TldE protein, part of TldE/TldD proteolytic complex, at 1,219,777 to 1,221,123
_1090
Position (kb)
1217
1218
1219
1220 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1216.784 kb on + strand, within Pf1N1B4_1086 at 1216.784 kb on + strand, within Pf1N1B4_1086 at 1216.785 kb on - strand, within Pf1N1B4_1086 at 1216.785 kb on - strand, within Pf1N1B4_1086 at 1216.785 kb on - strand, within Pf1N1B4_1086 at 1216.788 kb on - strand, within Pf1N1B4_1086 at 1216.824 kb on + strand, within Pf1N1B4_1086 at 1217.192 kb on + strand, within Pf1N1B4_1086 at 1217.192 kb on + strand, within Pf1N1B4_1086 at 1217.193 kb on - strand, within Pf1N1B4_1086 at 1217.193 kb on - strand, within Pf1N1B4_1086 at 1217.193 kb on - strand, within Pf1N1B4_1086 at 1217.213 kb on + strand, within Pf1N1B4_1086 at 1217.261 kb on + strand at 1217.261 kb on + strand at 1217.286 kb on + strand at 1217.286 kb on + strand at 1217.286 kb on + strand at 1217.287 kb on - strand at 1217.287 kb on - strand at 1217.315 kb on + strand at 1217.315 kb on + strand at 1217.316 kb on - strand at 1217.316 kb on - strand at 1217.316 kb on - strand at 1217.418 kb on + strand, within Pf1N1B4_1087 at 1217.418 kb on + strand at 1217.418 kb on + strand at 1217.419 kb on - strand, within Pf1N1B4_1087 at 1217.489 kb on + strand, within Pf1N1B4_1087 at 1217.489 kb on + strand, within Pf1N1B4_1087 at 1217.570 kb on - strand, within Pf1N1B4_1087 at 1217.658 kb on + strand, within Pf1N1B4_1087 at 1217.658 kb on + strand, within Pf1N1B4_1087 at 1217.659 kb on - strand, within Pf1N1B4_1087 at 1217.659 kb on - strand, within Pf1N1B4_1087 at 1217.766 kb on + strand at 1217.767 kb on - strand at 1217.874 kb on + strand at 1217.874 kb on + strand at 1219.100 kb on + strand at 1219.100 kb on + strand at 1219.100 kb on + strand at 1219.100 kb on + strand at 1219.101 kb on - strand at 1219.101 kb on - strand at 1219.101 kb on - strand at 1219.101 kb on - strand at 1219.101 kb on - strand at 1219.101 kb on - strand at 1219.101 kb on - strand at 1219.135 kb on + strand at 1219.164 kb on + strand at 1219.164 kb on + strand at 1219.164 kb on + strand at 1219.164 kb on + strand at 1219.164 kb on + strand at 1219.164 kb on + strand at 1219.164 kb on + strand at 1219.164 kb on + strand at 1219.213 kb on + strand at 1219.501 kb on + strand at 1219.501 kb on + strand at 1219.502 kb on - strand at 1219.640 kb on + strand at 1219.641 kb on - strand at 1219.721 kb on - strand at 1219.778 kb on + strand at 1219.778 kb on + strand at 1219.778 kb on + strand at 1219.778 kb on + strand at 1219.779 kb on - strand at 1219.779 kb on - strand at 1219.779 kb on - strand at 1219.779 kb on - strand at 1219.779 kb on - strand at 1219.783 kb on - strand at 1219.828 kb on - strand at 1219.828 kb on - strand at 1219.851 kb on + strand at 1219.899 kb on + strand at 1219.967 kb on + strand, within Pf1N1B4_1090 at 1220.135 kb on + strand, within Pf1N1B4_1090 at 1220.136 kb on - strand, within Pf1N1B4_1090 at 1220.136 kb on - strand, within Pf1N1B4_1090
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 1,216,784 + Pf1N1B4_1086 0.16 -0.5 1,216,784 + Pf1N1B4_1086 0.16 +0.1 1,216,785 - Pf1N1B4_1086 0.17 -0.6 1,216,785 - Pf1N1B4_1086 0.17 +0.1 1,216,785 - Pf1N1B4_1086 0.17 +1.7 1,216,788 - Pf1N1B4_1086 0.17 +1.8 1,216,824 + Pf1N1B4_1086 0.23 -0.9 1,217,192 + Pf1N1B4_1086 0.83 +1.1 1,217,192 + Pf1N1B4_1086 0.83 +1.0 1,217,193 - Pf1N1B4_1086 0.83 -0.8 1,217,193 - Pf1N1B4_1086 0.83 +0.1 1,217,193 - Pf1N1B4_1086 0.83 -1.5 1,217,213 + Pf1N1B4_1086 0.86 +2.1 1,217,261 + +0.8 1,217,261 + +0.1 1,217,286 + -0.4 1,217,286 + -0.5 1,217,286 + -0.9 1,217,287 - -0.1 1,217,287 - +0.4 1,217,315 + -2.2 1,217,315 + +2.1 1,217,316 - +1.1 1,217,316 - +0.7 1,217,316 - -1.2 1,217,418 + Pf1N1B4_1087 0.27 +1.2 1,217,418 + -0.5 1,217,418 + +0.1 1,217,419 - Pf1N1B4_1087 0.28 -0.3 1,217,489 + Pf1N1B4_1087 0.44 +0.1 1,217,489 + Pf1N1B4_1087 0.44 -0.5 1,217,570 - Pf1N1B4_1087 0.62 +0.6 1,217,658 + Pf1N1B4_1087 0.82 +0.2 1,217,658 + Pf1N1B4_1087 0.82 -1.2 1,217,659 - Pf1N1B4_1087 0.82 +0.1 1,217,659 - Pf1N1B4_1087 0.82 -1.9 1,217,766 + +1.1 1,217,767 - -0.9 1,217,874 + +0.7 1,217,874 + +0.1 1,219,100 + -0.3 1,219,100 + -1.2 1,219,100 + +0.1 1,219,100 + -0.5 1,219,101 - -0.9 1,219,101 - +0.1 1,219,101 - -1.1 1,219,101 - -0.5 1,219,101 - -2.1 1,219,101 - +0.7 1,219,101 - -0.5 1,219,135 + +0.1 1,219,164 + -0.6 1,219,164 + +0.8 1,219,164 + +0.1 1,219,164 + +1.4 1,219,164 + +0.3 1,219,164 + +0.4 1,219,164 + -1.8 1,219,164 + +0.7 1,219,213 + +2.1 1,219,501 + -2.5 1,219,501 + +0.7 1,219,502 - +0.1 1,219,640 + -0.9 1,219,641 - -0.2 1,219,721 - -0.9 1,219,778 + +0.1 1,219,778 + +0.1 1,219,778 + +0.1 1,219,778 + -0.9 1,219,779 - -1.1 1,219,779 - -1.1 1,219,779 - -1.2 1,219,779 - +0.1 1,219,779 - +0.1 1,219,783 - -0.9 1,219,828 - +0.4 1,219,828 - +1.1 1,219,851 + +0.7 1,219,899 + +0.4 1,219,967 + Pf1N1B4_1090 0.14 -0.5 1,220,135 + Pf1N1B4_1090 0.27 -0.5 1,220,136 - Pf1N1B4_1090 0.27 +0.8 1,220,136 - Pf1N1B4_1090 0.27 +0.1
Or see this region's nucleotide sequence