Experiment: ICP1_phage_23.5_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_17025 and CSW01_17030 are separated by 355 nucleotides CSW01_17030 and CSW01_17035 are separated by 9 nucleotides CSW01_17035 and CSW01_17040 are separated by 292 nucleotides
CSW01_17025: CSW01_17025 - GGDEF domain-containing protein, at 495,859 to 497,097
_17025
CSW01_17030: CSW01_17030 - competence protein ComFB, at 497,453 to 497,827
_17030
CSW01_17035: CSW01_17035 - transcriptional regulator, at 497,837 to 498,115
_17035
CSW01_17040: CSW01_17040 - NAD(P)(+) transhydrogenase (Re/Si-specific) subunit alpha, at 498,408 to 499,946
_17040
Position (kb) 497
498
499 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 496.877 kb on + strand, within CSW01_17025 at 496.958 kb on + strand, within CSW01_17025 at 496.986 kb on + strand at 497.014 kb on + strand at 497.161 kb on + strand at 497.251 kb on - strand at 497.251 kb on - strand at 497.251 kb on - strand at 497.251 kb on - strand at 497.251 kb on - strand at 497.251 kb on - strand at 497.360 kb on + strand at 497.397 kb on + strand at 497.516 kb on + strand, within CSW01_17030 at 497.524 kb on - strand, within CSW01_17030 at 497.563 kb on - strand, within CSW01_17030 at 497.563 kb on - strand, within CSW01_17030 at 497.565 kb on + strand, within CSW01_17030 at 497.565 kb on + strand, within CSW01_17030 at 497.599 kb on - strand, within CSW01_17030 at 497.692 kb on + strand, within CSW01_17030 at 497.805 kb on + strand at 497.898 kb on - strand, within CSW01_17035 at 498.160 kb on + strand at 498.186 kb on - strand at 498.342 kb on + strand at 498.538 kb on + strand at 498.771 kb on - strand, within CSW01_17040 at 498.820 kb on - strand, within CSW01_17040 at 498.833 kb on + strand, within CSW01_17040 at 498.897 kb on - strand, within CSW01_17040 at 499.074 kb on + strand, within CSW01_17040
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_23.5_MOI_rep2 remove 496,877 + CSW01_17025 0.82 -2.7 496,958 + CSW01_17025 0.89 +0.4 496,986 + +1.2 497,014 + -2.6 497,161 + +1.1 497,251 - -1.1 497,251 - -0.9 497,251 - +1.4 497,251 - +0.5 497,251 - +0.6 497,251 - -1.6 497,360 + -0.2 497,397 + +0.5 497,516 + CSW01_17030 0.17 -0.9 497,524 - CSW01_17030 0.19 -0.4 497,563 - CSW01_17030 0.29 +0.2 497,563 - CSW01_17030 0.29 -0.4 497,565 + CSW01_17030 0.30 -0.5 497,565 + CSW01_17030 0.30 -1.7 497,599 - CSW01_17030 0.39 +1.9 497,692 + CSW01_17030 0.64 +0.5 497,805 + +0.9 497,898 - CSW01_17035 0.22 -0.5 498,160 + -0.4 498,186 - -1.6 498,342 + +0.8 498,538 + -1.8 498,771 - CSW01_17040 0.24 -0.1 498,820 - CSW01_17040 0.27 -0.3 498,833 + CSW01_17040 0.28 +1.1 498,897 - CSW01_17040 0.32 -3.2 499,074 + CSW01_17040 0.43 -0.1
Or see this region's nucleotide sequence