Experiment: ICP1_phage_23.5_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_16150 and CSW01_16155 are separated by 147 nucleotides CSW01_16155 and CSW01_16160 are separated by 205 nucleotides CSW01_16160 and CSW01_16165 overlap by 89 nucleotides CSW01_16165 and CSW01_16170 are separated by 178 nucleotides CSW01_16170 and CSW01_16175 are separated by 161 nucleotides
CSW01_16150: CSW01_16150 - hypothetical protein, at 375,496 to 376,116
_16150
CSW01_16155: CSW01_16155 - lipocalin, at 376,264 to 376,779
_16155
CSW01_16160: CSW01_16160 - hypothetical protein, at 376,985 to 377,170
_16160
CSW01_16165: CSW01_16165 - DUF645 domain-containing protein, at 377,082 to 377,264
_16165
CSW01_16170: CSW01_16170 - hypothetical protein, at 377,443 to 377,790
_16170
CSW01_16175: CSW01_16175 - hypothetical protein, at 377,952 to 378,410
_16175
Position (kb) 377
378 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 376.084 kb on - strand at 376.084 kb on - strand at 376.142 kb on - strand at 376.142 kb on - strand at 376.939 kb on + strand at 377.084 kb on - strand at 377.085 kb on - strand at 377.193 kb on - strand, within CSW01_16165 at 378.042 kb on + strand, within CSW01_16175 at 378.078 kb on + strand, within CSW01_16175 at 378.109 kb on - strand, within CSW01_16175 at 378.113 kb on + strand, within CSW01_16175 at 378.113 kb on + strand, within CSW01_16175 at 378.113 kb on + strand, within CSW01_16175 at 378.113 kb on + strand, within CSW01_16175 at 378.113 kb on + strand, within CSW01_16175 at 378.120 kb on + strand, within CSW01_16175 at 378.130 kb on + strand, within CSW01_16175 at 378.182 kb on + strand, within CSW01_16175 at 378.190 kb on - strand, within CSW01_16175 at 378.190 kb on - strand, within CSW01_16175 at 378.190 kb on - strand, within CSW01_16175 at 378.190 kb on - strand, within CSW01_16175 at 378.257 kb on - strand, within CSW01_16175
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_23.5_MOI_rep2 remove 376,084 - +1.3 376,084 - -0.7 376,142 - -0.4 376,142 - -1.4 376,939 + -0.6 377,084 - +1.1 377,085 - -0.9 377,193 - CSW01_16165 0.61 +0.1 378,042 + CSW01_16175 0.20 +0.1 378,078 + CSW01_16175 0.27 -0.5 378,109 - CSW01_16175 0.34 -2.3 378,113 + CSW01_16175 0.35 +0.2 378,113 + CSW01_16175 0.35 -0.5 378,113 + CSW01_16175 0.35 -0.3 378,113 + CSW01_16175 0.35 -3.2 378,113 + CSW01_16175 0.35 -3.1 378,120 + CSW01_16175 0.37 +1.2 378,130 + CSW01_16175 0.39 +0.6 378,182 + CSW01_16175 0.50 -2.0 378,190 - CSW01_16175 0.52 +0.2 378,190 - CSW01_16175 0.52 -2.4 378,190 - CSW01_16175 0.52 +0.4 378,190 - CSW01_16175 0.52 +0.5 378,257 - CSW01_16175 0.66 +1.1
Or see this region's nucleotide sequence