Experiment: ICP1_phage_23.5_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_16035 and CSW01_16040 are separated by 200 nucleotides CSW01_16040 and CSW01_16045 are separated by 10 nucleotides CSW01_16045 and CSW01_16050 overlap by 85 nucleotides CSW01_16050 and CSW01_16055 are separated by 176 nucleotides CSW01_16055 and CSW01_16060 overlap by 38 nucleotides CSW01_16060 and CSW01_16065 are separated by 16 nucleotides CSW01_16065 and CSW01_16070 overlap by 19 nucleotides
CSW01_16035: CSW01_16035 - DUF645 domain-containing protein, at 367,160 to 367,340
_16035
CSW01_16040: CSW01_16040 - type II toxin-antitoxin system RelE/ParE family toxin, at 367,541 to 367,894
_16040
CSW01_16045: CSW01_16045 - transcriptional regulator, at 367,905 to 368,222
_16045
CSW01_16050: CSW01_16050 - DUF3265 domain-containing protein, at 368,138 to 368,329
_16050
CSW01_16055: CSW01_16055 - DUF645 domain-containing protein, at 368,506 to 368,631
_16055
CSW01_16060: CSW01_16060 - Tfp pilus assembly protein, at 368,594 to 368,710
_16060
CSW01_16065: CSW01_16065 - hypothetical protein, at 368,727 to 368,828
_16065
CSW01_16070: CSW01_16070 - isomerase, at 368,810 to 369,649
_16070
Position (kb)
367
368
369 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 367.241 kb on + strand, within CSW01_16035 at 367.241 kb on + strand, within CSW01_16035 at 367.241 kb on + strand, within CSW01_16035 at 367.249 kb on - strand, within CSW01_16035 at 367.249 kb on - strand, within CSW01_16035 at 367.249 kb on - strand, within CSW01_16035 at 367.249 kb on - strand, within CSW01_16035 at 367.479 kb on - strand at 367.649 kb on - strand, within CSW01_16040 at 367.668 kb on + strand, within CSW01_16040 at 367.668 kb on + strand, within CSW01_16040 at 367.727 kb on + strand, within CSW01_16040 at 367.775 kb on - strand, within CSW01_16040 at 368.946 kb on + strand, within CSW01_16070 at 369.005 kb on + strand, within CSW01_16070 at 369.021 kb on - strand, within CSW01_16070 at 369.057 kb on - strand, within CSW01_16070 at 369.113 kb on - strand, within CSW01_16070
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_23.5_MOI_rep2 remove 367,241 + CSW01_16035 0.45 +0.0 367,241 + CSW01_16035 0.45 +0.0 367,241 + CSW01_16035 0.45 -1.5 367,249 - CSW01_16035 0.49 -0.7 367,249 - CSW01_16035 0.49 +0.5 367,249 - CSW01_16035 0.49 -0.7 367,249 - CSW01_16035 0.49 +0.4 367,479 - +0.1 367,649 - CSW01_16040 0.31 +0.1 367,668 + CSW01_16040 0.36 -0.2 367,668 + CSW01_16040 0.36 -0.5 367,727 + CSW01_16040 0.53 -2.9 367,775 - CSW01_16040 0.66 -0.3 368,946 + CSW01_16070 0.16 -1.4 369,005 + CSW01_16070 0.23 +0.8 369,021 - CSW01_16070 0.25 -2.3 369,057 - CSW01_16070 0.29 -0.2 369,113 - CSW01_16070 0.36 -2.5
Or see this region's nucleotide sequence