Experiment: ICP1_phage_23.5_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_15010 and CSW01_15015 are separated by 189 nucleotides CSW01_15015 and CSW01_15020 are separated by 250 nucleotides
CSW01_15010: CSW01_15010 - ATP-dependent RNA helicase RhlE, at 220,762 to 222,117
_15010
CSW01_15015: CSW01_15015 - anaerobic C4-dicarboxylate transporter, at 222,307 to 223,635
_15015
CSW01_15020: CSW01_15020 - NAD(+) synthetase, at 223,886 to 224,716
_15020
Position (kb) 222
223
224 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 221.320 kb on - strand, within CSW01_15010 at 221.390 kb on + strand, within CSW01_15010 at 221.460 kb on + strand, within CSW01_15010 at 221.460 kb on + strand, within CSW01_15010 at 221.460 kb on + strand, within CSW01_15010 at 221.460 kb on + strand, within CSW01_15010 at 221.466 kb on - strand, within CSW01_15010 at 221.468 kb on - strand, within CSW01_15010 at 221.468 kb on - strand, within CSW01_15010 at 221.492 kb on - strand, within CSW01_15010 at 221.659 kb on - strand, within CSW01_15010 at 221.758 kb on - strand, within CSW01_15010 at 221.892 kb on - strand, within CSW01_15010 at 221.949 kb on + strand, within CSW01_15010 at 221.957 kb on - strand, within CSW01_15010 at 221.977 kb on + strand, within CSW01_15010 at 222.006 kb on - strand at 222.034 kb on - strand at 222.060 kb on - strand at 222.162 kb on + strand at 222.273 kb on + strand at 222.301 kb on - strand at 222.330 kb on + strand at 222.330 kb on + strand at 222.336 kb on + strand at 222.339 kb on + strand at 222.358 kb on - strand at 222.463 kb on + strand, within CSW01_15015 at 222.466 kb on - strand, within CSW01_15015 at 222.489 kb on - strand, within CSW01_15015 at 222.534 kb on - strand, within CSW01_15015 at 222.556 kb on - strand, within CSW01_15015 at 222.694 kb on + strand, within CSW01_15015 at 222.760 kb on - strand, within CSW01_15015 at 222.766 kb on + strand, within CSW01_15015 at 222.835 kb on + strand, within CSW01_15015 at 222.835 kb on + strand, within CSW01_15015 at 222.843 kb on - strand, within CSW01_15015 at 222.849 kb on + strand, within CSW01_15015 at 222.856 kb on - strand, within CSW01_15015 at 222.925 kb on - strand, within CSW01_15015 at 222.938 kb on - strand, within CSW01_15015 at 223.010 kb on + strand, within CSW01_15015 at 223.012 kb on + strand, within CSW01_15015 at 223.032 kb on + strand, within CSW01_15015 at 223.145 kb on - strand, within CSW01_15015 at 223.182 kb on - strand, within CSW01_15015 at 223.318 kb on + strand, within CSW01_15015 at 223.327 kb on - strand, within CSW01_15015 at 223.327 kb on - strand, within CSW01_15015 at 223.327 kb on - strand, within CSW01_15015 at 223.336 kb on + strand, within CSW01_15015 at 223.337 kb on - strand, within CSW01_15015 at 223.403 kb on - strand, within CSW01_15015 at 223.452 kb on + strand, within CSW01_15015 at 223.467 kb on + strand, within CSW01_15015 at 223.500 kb on - strand, within CSW01_15015 at 223.581 kb on + strand at 223.701 kb on + strand at 223.858 kb on - strand at 223.862 kb on + strand at 223.870 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_23.5_MOI_rep2 remove 221,320 - CSW01_15010 0.41 -2.6 221,390 + CSW01_15010 0.46 -1.7 221,460 + CSW01_15010 0.51 -0.8 221,460 + CSW01_15010 0.51 +1.1 221,460 + CSW01_15010 0.51 -1.2 221,460 + CSW01_15010 0.51 +0.8 221,466 - CSW01_15010 0.52 -3.3 221,468 - CSW01_15010 0.52 -2.7 221,468 - CSW01_15010 0.52 -3.2 221,492 - CSW01_15010 0.54 +1.8 221,659 - CSW01_15010 0.66 +0.4 221,758 - CSW01_15010 0.73 -0.4 221,892 - CSW01_15010 0.83 +0.6 221,949 + CSW01_15010 0.88 -0.3 221,957 - CSW01_15010 0.88 +1.3 221,977 + CSW01_15010 0.90 -2.9 222,006 - +1.2 222,034 - +0.6 222,060 - +0.8 222,162 + +0.5 222,273 + +0.1 222,301 - -3.0 222,330 + -0.7 222,330 + +0.6 222,336 + -0.3 222,339 + +0.4 222,358 - +1.4 222,463 + CSW01_15015 0.12 -1.4 222,466 - CSW01_15015 0.12 -2.2 222,489 - CSW01_15015 0.14 -0.4 222,534 - CSW01_15015 0.17 +0.3 222,556 - CSW01_15015 0.19 -0.5 222,694 + CSW01_15015 0.29 +0.6 222,760 - CSW01_15015 0.34 -2.9 222,766 + CSW01_15015 0.35 -4.2 222,835 + CSW01_15015 0.40 +0.6 222,835 + CSW01_15015 0.40 -0.1 222,843 - CSW01_15015 0.40 +0.2 222,849 + CSW01_15015 0.41 +2.3 222,856 - CSW01_15015 0.41 +1.2 222,925 - CSW01_15015 0.47 +1.1 222,938 - CSW01_15015 0.47 +0.8 223,010 + CSW01_15015 0.53 -0.2 223,012 + CSW01_15015 0.53 -0.1 223,032 + CSW01_15015 0.55 +0.4 223,145 - CSW01_15015 0.63 -1.6 223,182 - CSW01_15015 0.66 -1.3 223,318 + CSW01_15015 0.76 +0.2 223,327 - CSW01_15015 0.77 -0.4 223,327 - CSW01_15015 0.77 +0.3 223,327 - CSW01_15015 0.77 -1.7 223,336 + CSW01_15015 0.77 -1.9 223,337 - CSW01_15015 0.78 -2.9 223,403 - CSW01_15015 0.82 -2.1 223,452 + CSW01_15015 0.86 +0.3 223,467 + CSW01_15015 0.87 +1.2 223,500 - CSW01_15015 0.90 +2.8 223,581 + -0.8 223,701 + +0.0 223,858 - +0.4 223,862 + +0.4 223,870 - +0.2
Or see this region's nucleotide sequence