Experiment: ICP1_phage_23.5_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_12605 and CSW01_12610 are separated by 15 nucleotides CSW01_12610 and CSW01_12615 are separated by 100 nucleotides CSW01_12615 and CSW01_12620 are separated by 58 nucleotides CSW01_12620 and CSW01_12625 are separated by 42 nucleotides CSW01_12625 and CSW01_12630 are separated by 48 nucleotides CSW01_12630 and CSW01_12635 are separated by 107 nucleotides
CSW01_12605: CSW01_12605 - 3-isopropylmalate dehydratase large subunit, at 2,704,407 to 2,705,810
_12605
CSW01_12610: CSW01_12610 - 3-isopropylmalate dehydratase small subunit, at 2,705,826 to 2,706,428
_12610
CSW01_12615: CSW01_12615 - DUF547 domain-containing protein, at 2,706,529 to 2,707,320
_12615
CSW01_12620: CSW01_12620 - 5S ribosomal RNA, at 2,707,379 to 2,707,494
_12620
CSW01_12625: CSW01_12625 - tRNA-Thr, at 2,707,537 to 2,707,612
_12625
CSW01_12630: CSW01_12630 - 5S ribosomal RNA, at 2,707,661 to 2,707,776
_12630
CSW01_12635: CSW01_12635 - 23S ribosomal RNA, at 2,707,884 to 2,710,799
_12635
Position (kb)
2706
2707
2708 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2705.594 kb on - strand, within CSW01_12605 at 2705.723 kb on - strand at 2705.747 kb on - strand at 2705.804 kb on + strand at 2705.902 kb on - strand, within CSW01_12610 at 2705.959 kb on - strand, within CSW01_12610 at 2706.137 kb on - strand, within CSW01_12610 at 2706.358 kb on - strand, within CSW01_12610 at 2706.395 kb on + strand at 2706.403 kb on - strand at 2706.403 kb on - strand at 2706.597 kb on + strand at 2706.597 kb on + strand at 2706.599 kb on - strand at 2706.829 kb on - strand, within CSW01_12615 at 2706.946 kb on + strand, within CSW01_12615 at 2706.961 kb on - strand, within CSW01_12615 at 2707.021 kb on - strand, within CSW01_12615 at 2707.026 kb on + strand, within CSW01_12615 at 2707.044 kb on - strand, within CSW01_12615 at 2707.044 kb on - strand, within CSW01_12615 at 2707.054 kb on - strand, within CSW01_12615 at 2707.108 kb on + strand, within CSW01_12615 at 2707.115 kb on + strand, within CSW01_12615 at 2707.231 kb on + strand, within CSW01_12615 at 2707.236 kb on + strand, within CSW01_12615 at 2707.247 kb on + strand at 2707.249 kb on - strand at 2707.294 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_23.5_MOI_rep2 remove 2,705,594 - CSW01_12605 0.85 -3.3 2,705,723 - -0.4 2,705,747 - -0.3 2,705,804 + +2.4 2,705,902 - CSW01_12610 0.13 -0.2 2,705,959 - CSW01_12610 0.22 +1.8 2,706,137 - CSW01_12610 0.52 -1.0 2,706,358 - CSW01_12610 0.88 -2.9 2,706,395 + -0.2 2,706,403 - -3.3 2,706,403 - -2.3 2,706,597 + +0.9 2,706,597 + -1.1 2,706,599 - +1.1 2,706,829 - CSW01_12615 0.38 -0.1 2,706,946 + CSW01_12615 0.53 -0.6 2,706,961 - CSW01_12615 0.55 +0.8 2,707,021 - CSW01_12615 0.62 +1.4 2,707,026 + CSW01_12615 0.63 -0.1 2,707,044 - CSW01_12615 0.65 +0.7 2,707,044 - CSW01_12615 0.65 -2.7 2,707,054 - CSW01_12615 0.66 +0.3 2,707,108 + CSW01_12615 0.73 +1.0 2,707,115 + CSW01_12615 0.74 -0.9 2,707,231 + CSW01_12615 0.89 +0.0 2,707,236 + CSW01_12615 0.89 +1.8 2,707,247 + -0.1 2,707,249 - -0.7 2,707,294 - +1.5
Or see this region's nucleotide sequence