Strain Fitness in Vibrio cholerae E7946 ATCC 55056 around CSW01_11965

Experiment: ICP1_phage_23.5_MOI_rep2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCSW01_11955 and CSW01_11960 are separated by 345 nucleotidesCSW01_11960 and CSW01_11965 are separated by 79 nucleotidesCSW01_11965 and CSW01_11970 overlap by 4 nucleotidesCSW01_11970 and CSW01_11975 are separated by 20 nucleotides CSW01_11955: CSW01_11955 - endonuclease, at 2,547,332 to 2,548,192 _11955 CSW01_11960: CSW01_11960 - autonomous glycyl radical cofactor GrcA, at 2,548,538 to 2,548,915 _11960 CSW01_11965: CSW01_11965 - threonine synthase, at 2,548,995 to 2,550,275 _11965 CSW01_11970: CSW01_11970 - homoserine kinase, at 2,550,272 to 2,551,228 _11970 CSW01_11975: CSW01_11975 - bifunctional aspartate kinase/homoserine dehydrogenase I, at 2,551,249 to 2,553,708 _11975 Position (kb) 2548 2549 2550 2551Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2548.044 kb on - strand, within CSW01_11955at 2548.068 kb on - strand, within CSW01_11955at 2548.096 kb on + strand, within CSW01_11955at 2548.157 kb on + strandat 2548.200 kb on - strandat 2548.543 kb on - strandat 2548.582 kb on + strand, within CSW01_11960at 2548.590 kb on + strand, within CSW01_11960at 2548.629 kb on + strand, within CSW01_11960at 2548.658 kb on + strand, within CSW01_11960at 2548.706 kb on - strand, within CSW01_11960at 2548.776 kb on + strand, within CSW01_11960at 2548.865 kb on + strand, within CSW01_11960at 2549.038 kb on + strandat 2549.053 kb on - strandat 2549.058 kb on - strandat 2549.176 kb on - strand, within CSW01_11965at 2549.244 kb on - strand, within CSW01_11965at 2549.296 kb on + strand, within CSW01_11965at 2549.439 kb on + strand, within CSW01_11965at 2549.493 kb on + strand, within CSW01_11965at 2549.493 kb on + strand, within CSW01_11965at 2549.501 kb on - strand, within CSW01_11965at 2549.501 kb on - strand, within CSW01_11965at 2549.501 kb on - strand, within CSW01_11965at 2549.501 kb on - strand, within CSW01_11965at 2549.501 kb on - strand, within CSW01_11965at 2549.521 kb on - strand, within CSW01_11965at 2549.583 kb on - strand, within CSW01_11965at 2549.655 kb on + strand, within CSW01_11965at 2549.655 kb on + strand, within CSW01_11965at 2549.663 kb on - strand, within CSW01_11965at 2549.663 kb on - strand, within CSW01_11965at 2549.663 kb on - strand, within CSW01_11965at 2549.663 kb on - strand, within CSW01_11965at 2549.663 kb on - strand, within CSW01_11965at 2549.663 kb on - strand, within CSW01_11965at 2549.663 kb on - strand, within CSW01_11965at 2549.663 kb on - strand, within CSW01_11965at 2549.663 kb on - strand, within CSW01_11965at 2549.663 kb on - strand, within CSW01_11965at 2549.673 kb on + strand, within CSW01_11965at 2549.678 kb on + strand, within CSW01_11965at 2549.681 kb on - strand, within CSW01_11965at 2550.204 kb on + strandat 2550.204 kb on + strandat 2550.204 kb on + strandat 2550.204 kb on + strandat 2550.212 kb on - strandat 2550.212 kb on - strandat 2550.212 kb on - strandat 2550.259 kb on + strandat 2550.280 kb on - strandat 2550.334 kb on - strandat 2550.371 kb on + strand, within CSW01_11970at 2550.379 kb on - strand, within CSW01_11970at 2550.410 kb on + strand, within CSW01_11970at 2550.473 kb on - strand, within CSW01_11970at 2550.489 kb on + strand, within CSW01_11970at 2550.520 kb on - strand, within CSW01_11970at 2550.673 kb on + strand, within CSW01_11970at 2550.703 kb on + strand, within CSW01_11970at 2550.742 kb on + strand, within CSW01_11970at 2550.762 kb on - strand, within CSW01_11970at 2550.781 kb on - strand, within CSW01_11970at 2550.781 kb on - strand, within CSW01_11970at 2550.887 kb on + strand, within CSW01_11970at 2550.887 kb on + strand, within CSW01_11970at 2550.895 kb on - strand, within CSW01_11970at 2551.012 kb on - strand, within CSW01_11970at 2551.020 kb on + strand, within CSW01_11970at 2551.020 kb on - strand, within CSW01_11970at 2551.146 kb on + strandat 2551.163 kb on + strandat 2551.169 kb on - strandat 2551.171 kb on + strandat 2551.171 kb on + strandat 2551.179 kb on - strandat 2551.214 kb on - strandat 2551.224 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction ICP1_phage_23.5_MOI_rep2
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2,548,044 - CSW01_11955 0.83 +0.1
2,548,068 - CSW01_11955 0.85 -0.9
2,548,096 + CSW01_11955 0.89 +0.2
2,548,157 + +0.0
2,548,200 - +1.4
2,548,543 - +1.1
2,548,582 + CSW01_11960 0.12 +0.8
2,548,590 + CSW01_11960 0.14 +1.2
2,548,629 + CSW01_11960 0.24 -2.7
2,548,658 + CSW01_11960 0.32 +0.5
2,548,706 - CSW01_11960 0.44 -0.9
2,548,776 + CSW01_11960 0.63 -2.2
2,548,865 + CSW01_11960 0.87 +1.3
2,549,038 + -2.7
2,549,053 - -0.4
2,549,058 - +1.5
2,549,176 - CSW01_11965 0.14 +1.0
2,549,244 - CSW01_11965 0.19 +0.7
2,549,296 + CSW01_11965 0.23 +1.1
2,549,439 + CSW01_11965 0.35 -0.7
2,549,493 + CSW01_11965 0.39 -2.4
2,549,493 + CSW01_11965 0.39 +0.4
2,549,501 - CSW01_11965 0.40 +0.2
2,549,501 - CSW01_11965 0.40 -2.2
2,549,501 - CSW01_11965 0.40 -0.8
2,549,501 - CSW01_11965 0.40 +1.4
2,549,501 - CSW01_11965 0.40 -1.2
2,549,521 - CSW01_11965 0.41 -3.1
2,549,583 - CSW01_11965 0.46 +0.8
2,549,655 + CSW01_11965 0.52 +0.9
2,549,655 + CSW01_11965 0.52 +1.8
2,549,663 - CSW01_11965 0.52 +1.1
2,549,663 - CSW01_11965 0.52 +1.0
2,549,663 - CSW01_11965 0.52 -0.3
2,549,663 - CSW01_11965 0.52 +1.3
2,549,663 - CSW01_11965 0.52 +0.3
2,549,663 - CSW01_11965 0.52 +0.9
2,549,663 - CSW01_11965 0.52 -2.7
2,549,663 - CSW01_11965 0.52 +1.0
2,549,663 - CSW01_11965 0.52 -2.8
2,549,663 - CSW01_11965 0.52 +0.6
2,549,673 + CSW01_11965 0.53 +1.7
2,549,678 + CSW01_11965 0.53 -0.7
2,549,681 - CSW01_11965 0.54 +2.4
2,550,204 + +0.6
2,550,204 + -0.5
2,550,204 + -3.9
2,550,204 + +0.2
2,550,212 - +0.0
2,550,212 - -1.9
2,550,212 - -0.0
2,550,259 + -3.8
2,550,280 - -1.6
2,550,334 - -0.6
2,550,371 + CSW01_11970 0.10 +1.1
2,550,379 - CSW01_11970 0.11 +1.1
2,550,410 + CSW01_11970 0.14 -2.5
2,550,473 - CSW01_11970 0.21 -2.9
2,550,489 + CSW01_11970 0.23 -3.0
2,550,520 - CSW01_11970 0.26 -3.6
2,550,673 + CSW01_11970 0.42 -1.6
2,550,703 + CSW01_11970 0.45 +2.7
2,550,742 + CSW01_11970 0.49 -1.0
2,550,762 - CSW01_11970 0.51 +1.3
2,550,781 - CSW01_11970 0.53 -0.1
2,550,781 - CSW01_11970 0.53 -1.4
2,550,887 + CSW01_11970 0.64 +1.4
2,550,887 + CSW01_11970 0.64 +0.7
2,550,895 - CSW01_11970 0.65 -0.3
2,551,012 - CSW01_11970 0.77 -0.1
2,551,020 + CSW01_11970 0.78 -0.0
2,551,020 - CSW01_11970 0.78 -0.3
2,551,146 + -2.4
2,551,163 + -2.6
2,551,169 - -0.3
2,551,171 + +0.8
2,551,171 + +1.1
2,551,179 - +0.0
2,551,214 - +1.2
2,551,224 - -2.2

Or see this region's nucleotide sequence