Experiment: ICP1_phage_23.5_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_03720 and CSW01_03725 are separated by 107 nucleotides CSW01_03725 and CSW01_03730 are separated by 77 nucleotides CSW01_03730 and CSW01_03735 are separated by 193 nucleotides CSW01_03735 and CSW01_03740 are separated by 9 nucleotides CSW01_03740 and CSW01_03745 are separated by 34 nucleotides
CSW01_03720: CSW01_03720 - 23S ribosomal RNA, at 763,479 to 766,393
_03720
CSW01_03725: CSW01_03725 - 5S ribosomal RNA, at 766,501 to 766,616
_03725
CSW01_03730: CSW01_03730 - tRNA-Asp, at 766,694 to 766,770
_03730
CSW01_03735: CSW01_03735 - DUF1904 domain-containing protein, at 766,964 to 767,287
_03735
CSW01_03740: CSW01_03740 - oxygen-insensitive NADPH nitroreductase, at 767,297 to 768,019
_03740
CSW01_03745: CSW01_03745 - ferredoxin, at 768,054 to 768,308
_03745
Position (kb) 766
767
768 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 766.866 kb on + strand at 766.988 kb on + strand at 766.996 kb on - strand at 767.021 kb on + strand, within CSW01_03735 at 767.047 kb on + strand, within CSW01_03735 at 767.055 kb on - strand, within CSW01_03735 at 767.055 kb on - strand, within CSW01_03735 at 767.091 kb on - strand, within CSW01_03735 at 767.179 kb on + strand, within CSW01_03735 at 767.224 kb on - strand, within CSW01_03735 at 767.236 kb on + strand, within CSW01_03735 at 767.236 kb on + strand, within CSW01_03735 at 767.244 kb on - strand, within CSW01_03735 at 767.244 kb on - strand, within CSW01_03735 at 767.244 kb on - strand, within CSW01_03735 at 767.251 kb on - strand, within CSW01_03735 at 767.291 kb on + strand at 767.572 kb on + strand, within CSW01_03740 at 767.663 kb on + strand, within CSW01_03740 at 767.671 kb on - strand, within CSW01_03740 at 767.747 kb on + strand, within CSW01_03740 at 767.921 kb on + strand, within CSW01_03740 at 768.008 kb on + strand at 768.027 kb on + strand at 768.253 kb on - strand, within CSW01_03745 at 768.255 kb on - strand, within CSW01_03745
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_23.5_MOI_rep2 remove 766,866 + +0.3 766,988 + -0.7 766,996 - +1.6 767,021 + CSW01_03735 0.18 +0.8 767,047 + CSW01_03735 0.26 -2.1 767,055 - CSW01_03735 0.28 -0.3 767,055 - CSW01_03735 0.28 +1.5 767,091 - CSW01_03735 0.39 +0.0 767,179 + CSW01_03735 0.66 +0.0 767,224 - CSW01_03735 0.80 -4.1 767,236 + CSW01_03735 0.84 +1.2 767,236 + CSW01_03735 0.84 -0.1 767,244 - CSW01_03735 0.86 +0.5 767,244 - CSW01_03735 0.86 -1.4 767,244 - CSW01_03735 0.86 -0.4 767,251 - CSW01_03735 0.89 +2.1 767,291 + -3.0 767,572 + CSW01_03740 0.38 +0.6 767,663 + CSW01_03740 0.51 +1.1 767,671 - CSW01_03740 0.52 +0.7 767,747 + CSW01_03740 0.62 +0.5 767,921 + CSW01_03740 0.86 -1.5 768,008 + +1.1 768,027 + +1.8 768,253 - CSW01_03745 0.78 -2.6 768,255 - CSW01_03745 0.79 +0.6
Or see this region's nucleotide sequence