Experiment: ICP1_phage_23.5_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_03475 and CSW01_03480 are separated by 87 nucleotides CSW01_03480 and CSW01_03485 are separated by 308 nucleotides
CSW01_03475: CSW01_03475 - ATP-dependent RNA helicase SrmB, at 704,331 to 705,602
_03475
CSW01_03480: CSW01_03480 - tRNA (adenosine(37)-N6)-methyltransferase TrmM, at 705,690 to 706,412
_03480
CSW01_03485: CSW01_03485 - branched-chain amino acid transport system II carrier protein, at 706,721 to 708,034
_03485
Position (kb) 705
706
707 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 705.012 kb on + strand, within CSW01_03475 at 705.037 kb on - strand, within CSW01_03475 at 705.037 kb on - strand, within CSW01_03475 at 705.147 kb on - strand, within CSW01_03475 at 705.186 kb on - strand, within CSW01_03475 at 705.205 kb on + strand, within CSW01_03475 at 705.237 kb on - strand, within CSW01_03475 at 705.385 kb on + strand, within CSW01_03475 at 705.387 kb on - strand, within CSW01_03475 at 705.468 kb on + strand, within CSW01_03475 at 705.468 kb on + strand, within CSW01_03475 at 705.468 kb on + strand, within CSW01_03475 at 705.476 kb on - strand at 705.476 kb on - strand at 705.614 kb on + strand at 705.635 kb on + strand at 705.739 kb on + strand at 705.739 kb on + strand at 705.739 kb on + strand at 705.739 kb on + strand at 705.739 kb on + strand at 705.739 kb on + strand at 705.739 kb on + strand at 705.746 kb on - strand at 705.747 kb on - strand at 705.747 kb on - strand at 705.747 kb on - strand at 705.747 kb on - strand at 705.747 kb on - strand at 705.801 kb on - strand, within CSW01_03480 at 705.839 kb on - strand, within CSW01_03480 at 705.870 kb on + strand, within CSW01_03480 at 705.885 kb on + strand, within CSW01_03480 at 705.893 kb on - strand, within CSW01_03480 at 705.899 kb on + strand, within CSW01_03480 at 705.931 kb on - strand, within CSW01_03480 at 705.956 kb on + strand, within CSW01_03480 at 705.956 kb on + strand, within CSW01_03480 at 705.964 kb on - strand, within CSW01_03480 at 706.015 kb on + strand, within CSW01_03480 at 706.133 kb on + strand, within CSW01_03480 at 706.138 kb on + strand, within CSW01_03480 at 706.141 kb on - strand, within CSW01_03480 at 706.146 kb on - strand, within CSW01_03480 at 706.227 kb on - strand, within CSW01_03480 at 706.244 kb on - strand, within CSW01_03480 at 706.258 kb on - strand, within CSW01_03480 at 706.335 kb on - strand, within CSW01_03480 at 706.368 kb on - strand at 706.638 kb on + strand at 706.663 kb on + strand at 706.706 kb on + strand at 706.712 kb on + strand at 706.761 kb on + strand at 706.762 kb on - strand at 706.825 kb on + strand at 706.837 kb on + strand at 706.845 kb on - strand at 706.848 kb on - strand at 706.967 kb on + strand, within CSW01_03485 at 706.975 kb on - strand, within CSW01_03485 at 706.975 kb on - strand, within CSW01_03485 at 706.992 kb on - strand, within CSW01_03485 at 707.027 kb on + strand, within CSW01_03485 at 707.027 kb on + strand, within CSW01_03485 at 707.117 kb on + strand, within CSW01_03485 at 707.123 kb on + strand, within CSW01_03485 at 707.173 kb on + strand, within CSW01_03485 at 707.194 kb on - strand, within CSW01_03485 at 707.208 kb on - strand, within CSW01_03485 at 707.210 kb on + strand, within CSW01_03485 at 707.240 kb on - strand, within CSW01_03485 at 707.284 kb on - strand, within CSW01_03485 at 707.308 kb on - strand, within CSW01_03485 at 707.310 kb on - strand, within CSW01_03485 at 707.372 kb on + strand, within CSW01_03485 at 707.381 kb on - strand, within CSW01_03485 at 707.412 kb on + strand, within CSW01_03485
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_23.5_MOI_rep2 remove 705,012 + CSW01_03475 0.54 -1.2 705,037 - CSW01_03475 0.56 +1.6 705,037 - CSW01_03475 0.56 +1.8 705,147 - CSW01_03475 0.64 +1.2 705,186 - CSW01_03475 0.67 -1.6 705,205 + CSW01_03475 0.69 -2.2 705,237 - CSW01_03475 0.71 -0.1 705,385 + CSW01_03475 0.83 -1.2 705,387 - CSW01_03475 0.83 -0.4 705,468 + CSW01_03475 0.89 -0.7 705,468 + CSW01_03475 0.89 +0.3 705,468 + CSW01_03475 0.89 +0.8 705,476 - -2.5 705,476 - -0.9 705,614 + +0.1 705,635 + -1.2 705,739 + +0.6 705,739 + +0.7 705,739 + +1.0 705,739 + +0.9 705,739 + -1.2 705,739 + -2.6 705,739 + +1.7 705,746 - -2.2 705,747 - -3.5 705,747 - -1.4 705,747 - +1.2 705,747 - -1.9 705,747 - -0.4 705,801 - CSW01_03480 0.15 +0.1 705,839 - CSW01_03480 0.21 +0.8 705,870 + CSW01_03480 0.25 +0.5 705,885 + CSW01_03480 0.27 -1.1 705,893 - CSW01_03480 0.28 +0.7 705,899 + CSW01_03480 0.29 +1.0 705,931 - CSW01_03480 0.33 +1.1 705,956 + CSW01_03480 0.37 -1.0 705,956 + CSW01_03480 0.37 -0.0 705,964 - CSW01_03480 0.38 +0.3 706,015 + CSW01_03480 0.45 -4.0 706,133 + CSW01_03480 0.61 -0.1 706,138 + CSW01_03480 0.62 +1.2 706,141 - CSW01_03480 0.62 +1.3 706,146 - CSW01_03480 0.63 -0.2 706,227 - CSW01_03480 0.74 +2.1 706,244 - CSW01_03480 0.77 -0.6 706,258 - CSW01_03480 0.79 +0.4 706,335 - CSW01_03480 0.89 +1.6 706,368 - -2.4 706,638 + +1.0 706,663 + +0.6 706,706 + +0.0 706,712 + -1.2 706,761 + -2.1 706,762 - +0.0 706,825 + +1.1 706,837 + -0.5 706,845 - +0.6 706,848 - -0.4 706,967 + CSW01_03485 0.19 -0.0 706,975 - CSW01_03485 0.19 -1.9 706,975 - CSW01_03485 0.19 +1.5 706,992 - CSW01_03485 0.21 -1.8 707,027 + CSW01_03485 0.23 +2.3 707,027 + CSW01_03485 0.23 +0.2 707,117 + CSW01_03485 0.30 -0.5 707,123 + CSW01_03485 0.31 -1.1 707,173 + CSW01_03485 0.34 +0.1 707,194 - CSW01_03485 0.36 +0.9 707,208 - CSW01_03485 0.37 -0.6 707,210 + CSW01_03485 0.37 -2.0 707,240 - CSW01_03485 0.39 -2.9 707,284 - CSW01_03485 0.43 +0.1 707,308 - CSW01_03485 0.45 -0.2 707,310 - CSW01_03485 0.45 +1.6 707,372 + CSW01_03485 0.50 -2.6 707,381 - CSW01_03485 0.50 -0.1 707,412 + CSW01_03485 0.53 +1.3
Or see this region's nucleotide sequence