Experiment: ICP1_phage_23.5_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_02735 and CSW01_02740 are separated by 165 nucleotides CSW01_02740 and CSW01_02745 are separated by 144 nucleotides CSW01_02745 and CSW01_02750 are separated by 98 nucleotides
CSW01_02735: CSW01_02735 - integrase, at 547,519 to 548,760
_02735
CSW01_02740: CSW01_02740 - tRNA-Met, at 548,926 to 549,001
_02740
CSW01_02745: CSW01_02745 - RNA polymerase sigma factor RpoD, at 549,146 to 551,023
_02745
CSW01_02750: CSW01_02750 - DNA primase, at 551,122 to 552,885
_02750
Position (kb) 549
550
551
552 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 548.176 kb on + strand, within CSW01_02735 at 548.177 kb on - strand, within CSW01_02735 at 548.177 kb on - strand, within CSW01_02735 at 548.244 kb on - strand, within CSW01_02735 at 548.354 kb on - strand, within CSW01_02735 at 548.392 kb on - strand, within CSW01_02735 at 548.483 kb on + strand, within CSW01_02735 at 548.491 kb on - strand, within CSW01_02735 at 548.493 kb on + strand, within CSW01_02735 at 548.630 kb on + strand, within CSW01_02735 at 548.664 kb on + strand at 548.678 kb on + strand at 548.782 kb on + strand at 548.826 kb on - strand at 548.894 kb on - strand at 549.063 kb on - strand at 549.110 kb on - strand at 549.117 kb on - strand at 551.119 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_23.5_MOI_rep2 remove 548,176 + CSW01_02735 0.53 +0.8 548,177 - CSW01_02735 0.53 +0.4 548,177 - CSW01_02735 0.53 -0.1 548,244 - CSW01_02735 0.58 -2.4 548,354 - CSW01_02735 0.67 -2.8 548,392 - CSW01_02735 0.70 -0.1 548,483 + CSW01_02735 0.78 +0.4 548,491 - CSW01_02735 0.78 -4.3 548,493 + CSW01_02735 0.78 -1.5 548,630 + CSW01_02735 0.89 -2.3 548,664 + +1.9 548,678 + -0.9 548,782 + -4.5 548,826 - -0.2 548,894 - -1.3 549,063 - +2.1 549,110 - -1.6 549,117 - -4.4 551,119 + -0.7
Or see this region's nucleotide sequence