Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1420 and Shewana3_1421 are separated by 128 nucleotides Shewana3_1421 and Shewana3_1422 are separated by 37 nucleotides Shewana3_1422 and Shewana3_1423 are separated by 65 nucleotides Shewana3_1423 and Shewana3_1424 are separated by 34 nucleotides
Shewana3_1420: Shewana3_1420 - transcriptional regulator Ada / DNA-O6-methylguanine--protein-cysteine S-methyltransferase / DNA-3-methyladenine glycosylase II (RefSeq), at 1,649,381 to 1,651,009
_1420
Shewana3_1421: Shewana3_1421 - methylated-DNA--protein-cysteine methyltransferase (RefSeq), at 1,651,138 to 1,651,704
_1421
Shewana3_1422: Shewana3_1422 - 4a-hydroxytetrahydrobiopterin dehydratase (RefSeq), at 1,651,742 to 1,653,025
_1422
Shewana3_1423: Shewana3_1423 - Dual specificity protein phosphatase (RefSeq), at 1,653,091 to 1,653,561
_1423
Shewana3_1424: Shewana3_1424 - phospholipase D/transphosphatidylase (RefSeq), at 1,653,596 to 1,655,230
_1424
Position (kb) 1651
1652
1653
1654 Strain fitness (log2 ratio) -1
0
1 at 1651.132 kb on + strand at 1651.146 kb on + strand at 1651.335 kb on + strand, within Shewana3_1421 at 1651.335 kb on + strand, within Shewana3_1421 at 1651.412 kb on + strand, within Shewana3_1421 at 1651.529 kb on - strand, within Shewana3_1421 at 1651.825 kb on - strand at 1652.577 kb on + strand, within Shewana3_1422 at 1653.177 kb on - strand, within Shewana3_1423 at 1653.191 kb on + strand, within Shewana3_1423 at 1653.357 kb on - strand, within Shewana3_1423 at 1653.400 kb on + strand, within Shewana3_1423 at 1653.817 kb on - strand, within Shewana3_1424
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,651,132 + -1.1 1,651,146 + -0.5 1,651,335 + Shewana3_1421 0.35 -0.1 1,651,335 + Shewana3_1421 0.35 -0.7 1,651,412 + Shewana3_1421 0.48 -0.5 1,651,529 - Shewana3_1421 0.69 +0.5 1,651,825 - +0.5 1,652,577 + Shewana3_1422 0.65 -0.5 1,653,177 - Shewana3_1423 0.18 -0.1 1,653,191 + Shewana3_1423 0.21 +0.2 1,653,357 - Shewana3_1423 0.56 +0.5 1,653,400 + Shewana3_1423 0.66 +0.1 1,653,817 - Shewana3_1424 0.14 +0.5
Or see this region's nucleotide sequence