Experiment: A22 0.000341796875 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_2591 and pyrE are separated by 43 nucleotides pyrE and Synpcc7942_2593 are separated by 99 nucleotides Synpcc7942_2593 and tRNA-Met are separated by 44 nucleotides tRNA-Met and Synpcc7942_2594 are separated by 81 nucleotides Synpcc7942_2594 and Synpcc7942_2595 are separated by 6 nucleotides
Synpcc7942_2591: Synpcc7942_2591 - hypothetical protein, at 2,674,605 to 2,675,273
_2591
Synpcc7942_2592: pyrE - orotate phosphoribosyltransferase, at 2,675,317 to 2,675,925
pyrE
Synpcc7942_2593: Synpcc7942_2593 - hypothetical protein, at 2,676,025 to 2,676,561
_2593
Synpcc7942_R0024: tRNA-Met - tRNA-Met, at 2,676,606 to 2,676,678
tRNA-Met
Synpcc7942_2594: Synpcc7942_2594 - hypothetical protein, at 2,676,760 to 2,677,371
_2594
Synpcc7942_2595: Synpcc7942_2595 - hypothetical protein, at 2,677,378 to 2,678,808
_2595
Position (kb)
2676
2677 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4
5 at 2675.031 kb on + strand, within Synpcc7942_2591 at 2675.032 kb on - strand, within Synpcc7942_2591 at 2675.073 kb on + strand, within Synpcc7942_2591 at 2675.087 kb on - strand, within Synpcc7942_2591 at 2675.109 kb on - strand, within Synpcc7942_2591 at 2675.178 kb on - strand, within Synpcc7942_2591 at 2675.250 kb on + strand at 2675.278 kb on + strand at 2675.280 kb on + strand at 2675.286 kb on - strand at 2675.288 kb on - strand at 2675.288 kb on - strand at 2675.288 kb on - strand at 2675.291 kb on + strand at 2675.291 kb on + strand at 2675.299 kb on - strand at 2675.299 kb on - strand at 2675.301 kb on + strand at 2675.960 kb on + strand at 2675.960 kb on + strand at 2675.968 kb on - strand at 2676.002 kb on + strand at 2676.028 kb on - strand at 2676.028 kb on - strand at 2676.054 kb on - strand at 2676.059 kb on - strand at 2676.062 kb on + strand at 2676.071 kb on + strand at 2676.076 kb on + strand at 2676.081 kb on + strand, within Synpcc7942_2593 at 2676.081 kb on + strand, within Synpcc7942_2593 at 2676.081 kb on + strand, within Synpcc7942_2593 at 2676.081 kb on + strand, within Synpcc7942_2593 at 2676.081 kb on + strand, within Synpcc7942_2593 at 2676.086 kb on + strand, within Synpcc7942_2593 at 2676.089 kb on - strand, within Synpcc7942_2593 at 2676.089 kb on - strand, within Synpcc7942_2593 at 2676.089 kb on - strand, within Synpcc7942_2593 at 2676.089 kb on - strand, within Synpcc7942_2593 at 2676.091 kb on + strand, within Synpcc7942_2593 at 2676.099 kb on - strand, within Synpcc7942_2593 at 2676.127 kb on + strand, within Synpcc7942_2593 at 2676.185 kb on + strand, within Synpcc7942_2593 at 2676.208 kb on + strand, within Synpcc7942_2593 at 2676.215 kb on - strand, within Synpcc7942_2593 at 2676.236 kb on - strand, within Synpcc7942_2593 at 2676.272 kb on + strand, within Synpcc7942_2593 at 2676.291 kb on - strand, within Synpcc7942_2593 at 2676.301 kb on + strand, within Synpcc7942_2593 at 2676.309 kb on - strand, within Synpcc7942_2593 at 2676.320 kb on + strand, within Synpcc7942_2593 at 2676.362 kb on + strand, within Synpcc7942_2593 at 2676.370 kb on - strand, within Synpcc7942_2593 at 2676.370 kb on - strand, within Synpcc7942_2593 at 2676.374 kb on + strand, within Synpcc7942_2593 at 2676.376 kb on + strand, within Synpcc7942_2593 at 2676.392 kb on + strand, within Synpcc7942_2593 at 2676.400 kb on - strand, within Synpcc7942_2593 at 2676.409 kb on - strand, within Synpcc7942_2593 at 2676.433 kb on + strand, within Synpcc7942_2593 at 2676.435 kb on + strand, within Synpcc7942_2593 at 2676.441 kb on - strand, within Synpcc7942_2593 at 2676.477 kb on + strand, within Synpcc7942_2593 at 2676.477 kb on + strand, within Synpcc7942_2593 at 2676.477 kb on + strand, within Synpcc7942_2593 at 2676.485 kb on - strand, within Synpcc7942_2593 at 2676.498 kb on + strand, within Synpcc7942_2593 at 2676.514 kb on + strand at 2676.563 kb on - strand at 2676.583 kb on - strand at 2676.697 kb on - strand at 2676.743 kb on - strand at 2676.761 kb on + strand at 2676.783 kb on + strand at 2676.799 kb on - strand at 2676.843 kb on + strand, within Synpcc7942_2594 at 2676.848 kb on - strand, within Synpcc7942_2594 at 2676.879 kb on + strand, within Synpcc7942_2594 at 2676.885 kb on + strand, within Synpcc7942_2594 at 2676.912 kb on + strand, within Synpcc7942_2594 at 2676.920 kb on - strand, within Synpcc7942_2594 at 2676.947 kb on - strand, within Synpcc7942_2594 at 2676.985 kb on - strand, within Synpcc7942_2594 at 2676.995 kb on - strand, within Synpcc7942_2594 at 2677.001 kb on - strand, within Synpcc7942_2594 at 2677.001 kb on - strand, within Synpcc7942_2594 at 2677.048 kb on + strand, within Synpcc7942_2594 at 2677.073 kb on + strand, within Synpcc7942_2594 at 2677.085 kb on - strand, within Synpcc7942_2594 at 2677.121 kb on + strand, within Synpcc7942_2594 at 2677.121 kb on + strand, within Synpcc7942_2594 at 2677.121 kb on + strand, within Synpcc7942_2594 at 2677.129 kb on - strand, within Synpcc7942_2594 at 2677.166 kb on + strand, within Synpcc7942_2594 at 2677.167 kb on + strand, within Synpcc7942_2594 at 2677.244 kb on - strand, within Synpcc7942_2594 at 2677.253 kb on + strand, within Synpcc7942_2594 at 2677.253 kb on + strand, within Synpcc7942_2594 at 2677.254 kb on - strand, within Synpcc7942_2594 at 2677.268 kb on - strand, within Synpcc7942_2594 at 2677.289 kb on - strand, within Synpcc7942_2594 at 2677.301 kb on + strand, within Synpcc7942_2594 at 2677.314 kb on - strand at 2677.314 kb on - strand at 2677.314 kb on - strand at 2677.316 kb on + strand at 2677.323 kb on - strand at 2677.329 kb on + strand at 2677.374 kb on + strand at 2677.383 kb on - strand at 2677.400 kb on + strand at 2677.403 kb on - strand at 2677.405 kb on + strand at 2677.408 kb on - strand at 2677.444 kb on - strand at 2677.444 kb on - strand at 2677.482 kb on + strand at 2677.489 kb on - strand at 2677.503 kb on + strand at 2677.511 kb on - strand at 2677.526 kb on - strand, within Synpcc7942_2595 at 2677.535 kb on - strand, within Synpcc7942_2595
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.000341796875 mg/ml remove 2,675,031 + Synpcc7942_2591 0.64 +0.0 2,675,032 - Synpcc7942_2591 0.64 -2.3 2,675,073 + Synpcc7942_2591 0.70 -0.5 2,675,087 - Synpcc7942_2591 0.72 -2.5 2,675,109 - Synpcc7942_2591 0.75 +4.4 2,675,178 - Synpcc7942_2591 0.86 +1.0 2,675,250 + -0.3 2,675,278 + -1.5 2,675,280 + -0.5 2,675,286 - +2.3 2,675,288 - +0.5 2,675,288 - +1.0 2,675,288 - -1.0 2,675,291 + -2.7 2,675,291 + -0.3 2,675,299 - +0.6 2,675,299 - +0.0 2,675,301 + -3.0 2,675,960 + -1.3 2,675,960 + +0.0 2,675,968 - +1.0 2,676,002 + +0.2 2,676,028 - -1.3 2,676,028 - +2.5 2,676,054 - -2.9 2,676,059 - -2.9 2,676,062 + -1.8 2,676,071 + -2.9 2,676,076 + -2.4 2,676,081 + Synpcc7942_2593 0.10 -1.7 2,676,081 + Synpcc7942_2593 0.10 +1.0 2,676,081 + Synpcc7942_2593 0.10 -5.1 2,676,081 + Synpcc7942_2593 0.10 -0.5 2,676,081 + Synpcc7942_2593 0.10 -1.3 2,676,086 + Synpcc7942_2593 0.11 -4.3 2,676,089 - Synpcc7942_2593 0.12 -2.7 2,676,089 - Synpcc7942_2593 0.12 +1.4 2,676,089 - Synpcc7942_2593 0.12 -1.5 2,676,089 - Synpcc7942_2593 0.12 -2.8 2,676,091 + Synpcc7942_2593 0.12 -1.5 2,676,099 - Synpcc7942_2593 0.14 -3.1 2,676,127 + Synpcc7942_2593 0.19 -0.2 2,676,185 + Synpcc7942_2593 0.30 -0.5 2,676,208 + Synpcc7942_2593 0.34 -2.3 2,676,215 - Synpcc7942_2593 0.35 -2.0 2,676,236 - Synpcc7942_2593 0.39 -0.5 2,676,272 + Synpcc7942_2593 0.46 -1.3 2,676,291 - Synpcc7942_2593 0.50 -0.5 2,676,301 + Synpcc7942_2593 0.51 +0.2 2,676,309 - Synpcc7942_2593 0.53 -0.8 2,676,320 + Synpcc7942_2593 0.55 -2.0 2,676,362 + Synpcc7942_2593 0.63 +1.0 2,676,370 - Synpcc7942_2593 0.64 +0.0 2,676,370 - Synpcc7942_2593 0.64 -2.1 2,676,374 + Synpcc7942_2593 0.65 +0.0 2,676,376 + Synpcc7942_2593 0.65 -0.5 2,676,392 + Synpcc7942_2593 0.68 -3.8 2,676,400 - Synpcc7942_2593 0.70 -5.1 2,676,409 - Synpcc7942_2593 0.72 -2.4 2,676,433 + Synpcc7942_2593 0.76 -3.2 2,676,435 + Synpcc7942_2593 0.76 -0.2 2,676,441 - Synpcc7942_2593 0.77 +0.1 2,676,477 + Synpcc7942_2593 0.84 -4.6 2,676,477 + Synpcc7942_2593 0.84 -1.8 2,676,477 + Synpcc7942_2593 0.84 -1.5 2,676,485 - Synpcc7942_2593 0.86 -1.6 2,676,498 + Synpcc7942_2593 0.88 -2.3 2,676,514 + -1.3 2,676,563 - -0.0 2,676,583 - -1.4 2,676,697 - -1.0 2,676,743 - -2.1 2,676,761 + -1.8 2,676,783 + -1.0 2,676,799 - -0.5 2,676,843 + Synpcc7942_2594 0.14 -1.5 2,676,848 - Synpcc7942_2594 0.14 +1.9 2,676,879 + Synpcc7942_2594 0.19 +0.6 2,676,885 + Synpcc7942_2594 0.20 -0.3 2,676,912 + Synpcc7942_2594 0.25 -1.3 2,676,920 - Synpcc7942_2594 0.26 -0.5 2,676,947 - Synpcc7942_2594 0.31 -3.7 2,676,985 - Synpcc7942_2594 0.37 -1.8 2,676,995 - Synpcc7942_2594 0.38 +1.0 2,677,001 - Synpcc7942_2594 0.39 -2.5 2,677,001 - Synpcc7942_2594 0.39 +0.9 2,677,048 + Synpcc7942_2594 0.47 -3.5 2,677,073 + Synpcc7942_2594 0.51 +0.0 2,677,085 - Synpcc7942_2594 0.53 -1.5 2,677,121 + Synpcc7942_2594 0.59 +0.5 2,677,121 + Synpcc7942_2594 0.59 -1.8 2,677,121 + Synpcc7942_2594 0.59 -1.0 2,677,129 - Synpcc7942_2594 0.60 -3.0 2,677,166 + Synpcc7942_2594 0.66 +0.4 2,677,167 + Synpcc7942_2594 0.67 -1.8 2,677,244 - Synpcc7942_2594 0.79 +1.2 2,677,253 + Synpcc7942_2594 0.81 -2.0 2,677,253 + Synpcc7942_2594 0.81 -1.0 2,677,254 - Synpcc7942_2594 0.81 +0.0 2,677,268 - Synpcc7942_2594 0.83 +2.6 2,677,289 - Synpcc7942_2594 0.86 -2.3 2,677,301 + Synpcc7942_2594 0.88 -1.0 2,677,314 - -0.5 2,677,314 - -1.0 2,677,314 - +0.0 2,677,316 + +3.5 2,677,323 - +0.0 2,677,329 + -0.5 2,677,374 + -1.8 2,677,383 - +5.7 2,677,400 + -1.0 2,677,403 - -0.5 2,677,405 + -2.7 2,677,408 - +2.5 2,677,444 - -2.9 2,677,444 - -2.0 2,677,482 + +1.0 2,677,489 - -2.4 2,677,503 + -2.0 2,677,511 - -1.3 2,677,526 - Synpcc7942_2595 0.10 +1.0 2,677,535 - Synpcc7942_2595 0.11 -2.5
Or see this region's nucleotide sequence