Experiment: A22 0.000341796875 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sulA and psb28-2 are separated by 15 nucleotides psb28-2 and Synpcc7942_2479 are separated by 500 nucleotides Synpcc7942_2479 and Synpcc7942_2480 are separated by 4 nucleotides Synpcc7942_2480 and Synpcc7942_2481 overlap by 13 nucleotides
Synpcc7942_2477: sulA - hypothetical protein, at 2,558,588 to 2,559,484
sulA
Synpcc7942_2478: psb28-2 - photosystem II reaction center W protein, at 2,559,500 to 2,559,874
psb28-2
Synpcc7942_2479: Synpcc7942_2479 - pilin-like protein, at 2,560,375 to 2,560,941
_2479
Synpcc7942_2480: Synpcc7942_2480 - Prolyl 4-hydroxylase, alpha subunit, at 2,560,946 to 2,561,848
_2480
Synpcc7942_2481: Synpcc7942_2481 - hypothetical protein, at 2,561,836 to 2,563,068
_2481
Position (kb)
2560
2561 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2559.406 kb on + strand at 2559.406 kb on + strand at 2559.414 kb on - strand at 2559.414 kb on - strand at 2559.415 kb on - strand at 2559.420 kb on + strand at 2559.428 kb on - strand at 2559.435 kb on + strand at 2559.487 kb on - strand at 2559.487 kb on - strand at 2559.505 kb on - strand at 2559.580 kb on + strand, within psb28-2 at 2559.614 kb on + strand, within psb28-2 at 2559.614 kb on + strand, within psb28-2 at 2559.614 kb on + strand, within psb28-2 at 2559.614 kb on + strand, within psb28-2 at 2559.622 kb on - strand, within psb28-2 at 2559.649 kb on + strand, within psb28-2 at 2559.649 kb on + strand, within psb28-2 at 2559.657 kb on - strand, within psb28-2 at 2559.668 kb on + strand, within psb28-2 at 2559.697 kb on + strand, within psb28-2 at 2559.737 kb on - strand, within psb28-2 at 2559.754 kb on + strand, within psb28-2 at 2559.767 kb on + strand, within psb28-2 at 2559.767 kb on + strand, within psb28-2 at 2559.767 kb on + strand, within psb28-2 at 2559.780 kb on - strand, within psb28-2 at 2559.815 kb on - strand, within psb28-2 at 2559.836 kb on + strand, within psb28-2 at 2559.852 kb on + strand at 2559.852 kb on + strand at 2559.860 kb on - strand at 2559.882 kb on + strand at 2559.890 kb on - strand at 2559.898 kb on + strand at 2559.898 kb on + strand at 2559.900 kb on - strand at 2560.027 kb on + strand at 2560.027 kb on + strand at 2560.033 kb on - strand at 2560.107 kb on - strand at 2560.119 kb on + strand at 2560.127 kb on - strand at 2560.127 kb on - strand at 2560.196 kb on - strand at 2560.306 kb on - strand at 2560.322 kb on + strand at 2560.325 kb on - strand at 2560.353 kb on + strand at 2560.417 kb on - strand at 2560.435 kb on + strand at 2560.443 kb on - strand, within Synpcc7942_2479 at 2560.527 kb on + strand, within Synpcc7942_2479 at 2560.527 kb on + strand, within Synpcc7942_2479 at 2560.533 kb on - strand, within Synpcc7942_2479 at 2560.579 kb on + strand, within Synpcc7942_2479 at 2560.582 kb on - strand, within Synpcc7942_2479 at 2560.662 kb on - strand, within Synpcc7942_2479 at 2560.671 kb on + strand, within Synpcc7942_2479 at 2560.711 kb on + strand, within Synpcc7942_2479 at 2560.721 kb on + strand, within Synpcc7942_2479 at 2560.729 kb on - strand, within Synpcc7942_2479 at 2560.786 kb on - strand, within Synpcc7942_2479 at 2560.855 kb on + strand, within Synpcc7942_2479 at 2560.882 kb on + strand, within Synpcc7942_2479 at 2560.890 kb on - strand at 2560.891 kb on - strand at 2560.891 kb on - strand at 2560.901 kb on - strand at 2560.901 kb on - strand at 2560.907 kb on - strand at 2560.978 kb on + strand at 2561.047 kb on - strand, within Synpcc7942_2480 at 2561.062 kb on + strand, within Synpcc7942_2480 at 2561.139 kb on - strand, within Synpcc7942_2480 at 2561.222 kb on - strand, within Synpcc7942_2480 at 2561.437 kb on - strand, within Synpcc7942_2480 at 2561.696 kb on + strand, within Synpcc7942_2480 at 2561.940 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.000341796875 mg/ml remove 2,559,406 + +0.0 2,559,406 + -2.0 2,559,414 - -1.3 2,559,414 - -2.0 2,559,415 - -1.3 2,559,420 + -2.0 2,559,428 - -1.5 2,559,435 + -2.0 2,559,487 - +1.0 2,559,487 - -0.5 2,559,505 - -1.5 2,559,580 + psb28-2 Synpcc7942_2478 0.21 -1.0 2,559,614 + psb28-2 Synpcc7942_2478 0.30 -1.5 2,559,614 + psb28-2 Synpcc7942_2478 0.30 -1.0 2,559,614 + psb28-2 Synpcc7942_2478 0.30 +1.0 2,559,614 + psb28-2 Synpcc7942_2478 0.30 +0.9 2,559,622 - psb28-2 Synpcc7942_2478 0.33 -2.9 2,559,649 + psb28-2 Synpcc7942_2478 0.40 -2.0 2,559,649 + psb28-2 Synpcc7942_2478 0.40 +3.2 2,559,657 - psb28-2 Synpcc7942_2478 0.42 -1.8 2,559,668 + psb28-2 Synpcc7942_2478 0.45 +1.5 2,559,697 + psb28-2 Synpcc7942_2478 0.53 +0.0 2,559,737 - psb28-2 Synpcc7942_2478 0.63 +1.0 2,559,754 + psb28-2 Synpcc7942_2478 0.68 -2.4 2,559,767 + psb28-2 Synpcc7942_2478 0.71 +0.9 2,559,767 + psb28-2 Synpcc7942_2478 0.71 -1.1 2,559,767 + psb28-2 Synpcc7942_2478 0.71 +0.0 2,559,780 - psb28-2 Synpcc7942_2478 0.75 -0.8 2,559,815 - psb28-2 Synpcc7942_2478 0.84 -1.8 2,559,836 + psb28-2 Synpcc7942_2478 0.90 -2.0 2,559,852 + +2.8 2,559,852 + +1.0 2,559,860 - +1.0 2,559,882 + +1.6 2,559,890 - +3.7 2,559,898 + +1.0 2,559,898 + +1.0 2,559,900 - +0.9 2,560,027 + -1.0 2,560,027 + -1.8 2,560,033 - -1.1 2,560,107 - -0.5 2,560,119 + -1.5 2,560,127 - -0.5 2,560,127 - -1.7 2,560,196 - -2.3 2,560,306 - -1.5 2,560,322 + -1.8 2,560,325 - -1.5 2,560,353 + -0.3 2,560,417 - -2.0 2,560,435 + +1.0 2,560,443 - Synpcc7942_2479 0.12 -2.1 2,560,527 + Synpcc7942_2479 0.27 -0.4 2,560,527 + Synpcc7942_2479 0.27 +0.0 2,560,533 - Synpcc7942_2479 0.28 -2.3 2,560,579 + Synpcc7942_2479 0.36 -0.1 2,560,582 - Synpcc7942_2479 0.37 -2.3 2,560,662 - Synpcc7942_2479 0.51 -2.5 2,560,671 + Synpcc7942_2479 0.52 -2.3 2,560,711 + Synpcc7942_2479 0.59 -0.8 2,560,721 + Synpcc7942_2479 0.61 -1.8 2,560,729 - Synpcc7942_2479 0.62 -2.1 2,560,786 - Synpcc7942_2479 0.72 -4.2 2,560,855 + Synpcc7942_2479 0.85 +0.9 2,560,882 + Synpcc7942_2479 0.89 +0.8 2,560,890 - -3.2 2,560,891 - -2.7 2,560,891 - -1.3 2,560,901 - +1.4 2,560,901 - +0.3 2,560,907 - -3.2 2,560,978 + -2.8 2,561,047 - Synpcc7942_2480 0.11 -2.1 2,561,062 + Synpcc7942_2480 0.13 -0.1 2,561,139 - Synpcc7942_2480 0.21 -1.3 2,561,222 - Synpcc7942_2480 0.31 -2.5 2,561,437 - Synpcc7942_2480 0.54 -1.0 2,561,696 + Synpcc7942_2480 0.83 -0.4 2,561,940 + -1.0
Or see this region's nucleotide sequence