Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS37085 and RR42_RS37090 are separated by 63 nucleotides RR42_RS37090 and RR42_RS37095 overlap by 4 nucleotides RR42_RS37095 and RR42_RS37105 are separated by 841 nucleotides
RR42_RS37085: RR42_RS37085 - ATP-dependent DNA ligase, at 3,783,682 to 3,786,183
_RS37085
RR42_RS37090: RR42_RS37090 - DNA repair protein, at 3,786,247 to 3,787,161
_RS37090
RR42_RS37095: RR42_RS37095 - hypothetical protein, at 3,787,158 to 3,787,994
_RS37095
RR42_RS37105: RR42_RS37105 - hypothetical protein, at 3,788,836 to 3,789,081
_RS37105
Position (kb)
3787
3788 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3786.168 kb on + strand at 3786.168 kb on + strand at 3786.169 kb on - strand at 3786.169 kb on - strand at 3786.243 kb on + strand at 3786.244 kb on - strand at 3786.244 kb on - strand at 3786.244 kb on - strand at 3786.246 kb on + strand at 3786.246 kb on + strand at 3786.246 kb on + strand at 3786.246 kb on + strand at 3786.247 kb on - strand at 3786.247 kb on - strand at 3786.311 kb on + strand at 3786.311 kb on + strand at 3786.312 kb on - strand at 3786.312 kb on - strand at 3786.312 kb on - strand at 3786.312 kb on - strand at 3786.314 kb on - strand at 3786.353 kb on - strand, within RR42_RS37090 at 3786.353 kb on - strand, within RR42_RS37090 at 3786.388 kb on + strand, within RR42_RS37090 at 3786.443 kb on - strand, within RR42_RS37090 at 3786.443 kb on - strand, within RR42_RS37090 at 3786.463 kb on + strand, within RR42_RS37090 at 3786.565 kb on + strand, within RR42_RS37090 at 3786.565 kb on + strand, within RR42_RS37090 at 3786.565 kb on + strand, within RR42_RS37090 at 3786.566 kb on - strand, within RR42_RS37090 at 3786.580 kb on + strand, within RR42_RS37090 at 3786.616 kb on + strand, within RR42_RS37090 at 3786.617 kb on - strand, within RR42_RS37090 at 3786.693 kb on + strand, within RR42_RS37090 at 3786.693 kb on + strand, within RR42_RS37090 at 3786.694 kb on - strand, within RR42_RS37090 at 3786.813 kb on - strand, within RR42_RS37090 at 3786.852 kb on + strand, within RR42_RS37090 at 3786.852 kb on + strand, within RR42_RS37090 at 3786.853 kb on - strand, within RR42_RS37090 at 3786.853 kb on - strand, within RR42_RS37090 at 3786.857 kb on - strand, within RR42_RS37090 at 3786.857 kb on - strand, within RR42_RS37090 at 3786.857 kb on - strand, within RR42_RS37090 at 3786.978 kb on + strand, within RR42_RS37090 at 3786.979 kb on - strand, within RR42_RS37090 at 3786.979 kb on - strand, within RR42_RS37090 at 3786.979 kb on - strand, within RR42_RS37090 at 3786.987 kb on + strand, within RR42_RS37090 at 3786.987 kb on + strand, within RR42_RS37090 at 3786.987 kb on + strand, within RR42_RS37090 at 3787.185 kb on + strand at 3787.185 kb on + strand at 3787.185 kb on + strand at 3787.186 kb on - strand at 3787.186 kb on - strand at 3787.201 kb on + strand at 3787.201 kb on + strand at 3787.226 kb on + strand at 3787.227 kb on - strand at 3787.227 kb on - strand at 3787.332 kb on + strand, within RR42_RS37095 at 3787.333 kb on - strand, within RR42_RS37095 at 3787.457 kb on + strand, within RR42_RS37095 at 3787.462 kb on + strand, within RR42_RS37095 at 3787.462 kb on + strand, within RR42_RS37095 at 3787.572 kb on - strand, within RR42_RS37095 at 3787.572 kb on - strand, within RR42_RS37095 at 3787.692 kb on + strand, within RR42_RS37095 at 3787.692 kb on + strand, within RR42_RS37095 at 3787.692 kb on + strand, within RR42_RS37095 at 3787.693 kb on - strand, within RR42_RS37095 at 3787.693 kb on - strand, within RR42_RS37095 at 3787.701 kb on + strand, within RR42_RS37095 at 3787.702 kb on - strand, within RR42_RS37095 at 3787.798 kb on + strand, within RR42_RS37095 at 3787.864 kb on - strand, within RR42_RS37095 at 3787.893 kb on + strand, within RR42_RS37095 at 3787.965 kb on + strand at 3787.966 kb on - strand at 3788.003 kb on + strand at 3788.004 kb on - strand at 3788.016 kb on + strand at 3788.016 kb on + strand at 3788.017 kb on - strand at 3788.017 kb on - strand at 3788.017 kb on - strand at 3788.056 kb on + strand at 3788.123 kb on + strand at 3788.123 kb on + strand at 3788.124 kb on - strand at 3788.246 kb on + strand at 3788.246 kb on + strand at 3788.341 kb on + strand at 3788.341 kb on + strand at 3788.342 kb on - strand at 3788.403 kb on + strand at 3788.453 kb on - strand at 3788.453 kb on - strand at 3788.500 kb on + strand at 3788.500 kb on + strand at 3788.500 kb on + strand at 3788.501 kb on - strand at 3788.549 kb on + strand at 3788.549 kb on + strand at 3788.549 kb on + strand at 3788.550 kb on - strand at 3788.632 kb on - strand at 3788.674 kb on + strand at 3788.737 kb on + strand at 3788.738 kb on - strand at 3788.738 kb on - strand at 3788.738 kb on - strand at 3788.738 kb on - strand at 3788.800 kb on - strand at 3788.800 kb on - strand at 3788.882 kb on + strand, within RR42_RS37105 at 3788.896 kb on + strand, within RR42_RS37105 at 3788.897 kb on + strand, within RR42_RS37105 at 3788.898 kb on - strand, within RR42_RS37105 at 3788.898 kb on - strand, within RR42_RS37105 at 3788.898 kb on - strand, within RR42_RS37105 at 3788.900 kb on + strand, within RR42_RS37105 at 3788.901 kb on - strand, within RR42_RS37105 at 3788.901 kb on - strand, within RR42_RS37105 at 3788.901 kb on - strand, within RR42_RS37105 at 3788.901 kb on - strand, within RR42_RS37105 at 3788.909 kb on + strand, within RR42_RS37105 at 3788.909 kb on + strand, within RR42_RS37105 at 3788.910 kb on - strand, within RR42_RS37105 at 3788.910 kb on - strand, within RR42_RS37105 at 3788.910 kb on - strand, within RR42_RS37105 at 3788.910 kb on - strand, within RR42_RS37105 at 3788.910 kb on - strand, within RR42_RS37105 at 3788.912 kb on + strand, within RR42_RS37105 at 3788.912 kb on + strand, within RR42_RS37105 at 3788.913 kb on - strand, within RR42_RS37105 at 3788.955 kb on + strand, within RR42_RS37105 at 3788.955 kb on + strand, within RR42_RS37105 at 3788.956 kb on - strand, within RR42_RS37105
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 3,786,168 + +1.0 3,786,168 + +1.3 3,786,169 - +0.3 3,786,169 - -0.1 3,786,243 + +0.4 3,786,244 - -0.1 3,786,244 - +0.0 3,786,244 - +1.0 3,786,246 + -1.8 3,786,246 + -0.8 3,786,246 + -1.0 3,786,246 + -0.5 3,786,247 - -0.5 3,786,247 - -3.1 3,786,311 + -0.9 3,786,311 + -1.0 3,786,312 - -0.3 3,786,312 - +0.7 3,786,312 - -0.2 3,786,312 - -1.7 3,786,314 - +0.2 3,786,353 - RR42_RS37090 0.12 -1.1 3,786,353 - RR42_RS37090 0.12 -0.1 3,786,388 + RR42_RS37090 0.15 -1.8 3,786,443 - RR42_RS37090 0.21 -1.0 3,786,443 - RR42_RS37090 0.21 +0.1 3,786,463 + RR42_RS37090 0.24 -1.4 3,786,565 + RR42_RS37090 0.35 +0.1 3,786,565 + RR42_RS37090 0.35 +0.1 3,786,565 + RR42_RS37090 0.35 -0.7 3,786,566 - RR42_RS37090 0.35 +0.3 3,786,580 + RR42_RS37090 0.36 -1.3 3,786,616 + RR42_RS37090 0.40 +0.4 3,786,617 - RR42_RS37090 0.40 +0.6 3,786,693 + RR42_RS37090 0.49 -0.7 3,786,693 + RR42_RS37090 0.49 -1.9 3,786,694 - RR42_RS37090 0.49 +0.9 3,786,813 - RR42_RS37090 0.62 -0.3 3,786,852 + RR42_RS37090 0.66 +0.3 3,786,852 + RR42_RS37090 0.66 -0.3 3,786,853 - RR42_RS37090 0.66 -0.2 3,786,853 - RR42_RS37090 0.66 +0.5 3,786,857 - RR42_RS37090 0.67 +1.2 3,786,857 - RR42_RS37090 0.67 -0.4 3,786,857 - RR42_RS37090 0.67 -0.1 3,786,978 + RR42_RS37090 0.80 +0.3 3,786,979 - RR42_RS37090 0.80 +0.3 3,786,979 - RR42_RS37090 0.80 +1.4 3,786,979 - RR42_RS37090 0.80 -0.3 3,786,987 + RR42_RS37090 0.81 +0.3 3,786,987 + RR42_RS37090 0.81 +1.0 3,786,987 + RR42_RS37090 0.81 +2.4 3,787,185 + -0.0 3,787,185 + +0.9 3,787,185 + -1.1 3,787,186 - -0.5 3,787,186 - -0.0 3,787,201 + -0.8 3,787,201 + +1.1 3,787,226 + +3.4 3,787,227 - -0.3 3,787,227 - -0.1 3,787,332 + RR42_RS37095 0.21 -0.8 3,787,333 - RR42_RS37095 0.21 -0.2 3,787,457 + RR42_RS37095 0.36 -0.1 3,787,462 + RR42_RS37095 0.36 +0.1 3,787,462 + RR42_RS37095 0.36 -0.3 3,787,572 - RR42_RS37095 0.49 -2.5 3,787,572 - RR42_RS37095 0.49 -0.2 3,787,692 + RR42_RS37095 0.64 -1.8 3,787,692 + RR42_RS37095 0.64 +1.1 3,787,692 + RR42_RS37095 0.64 -0.2 3,787,693 - RR42_RS37095 0.64 +0.2 3,787,693 - RR42_RS37095 0.64 -1.9 3,787,701 + RR42_RS37095 0.65 -0.2 3,787,702 - RR42_RS37095 0.65 -0.0 3,787,798 + RR42_RS37095 0.76 +0.0 3,787,864 - RR42_RS37095 0.84 -0.4 3,787,893 + RR42_RS37095 0.88 +0.2 3,787,965 + -0.6 3,787,966 - -0.4 3,788,003 + +0.5 3,788,004 - +1.1 3,788,016 + -0.0 3,788,016 + +0.5 3,788,017 - -1.1 3,788,017 - -1.1 3,788,017 - -0.8 3,788,056 + -1.0 3,788,123 + -1.1 3,788,123 + -0.2 3,788,124 - +0.8 3,788,246 + +0.1 3,788,246 + -0.5 3,788,341 + -2.4 3,788,341 + +0.2 3,788,342 - -0.1 3,788,403 + -1.2 3,788,453 - +0.0 3,788,453 - +1.0 3,788,500 + +0.1 3,788,500 + -0.3 3,788,500 + +0.1 3,788,501 - +1.7 3,788,549 + +0.7 3,788,549 + -1.7 3,788,549 + -1.3 3,788,550 - -0.1 3,788,632 - -0.6 3,788,674 + +0.7 3,788,737 + +0.2 3,788,738 - +0.8 3,788,738 - -0.6 3,788,738 - -0.6 3,788,738 - -0.5 3,788,800 - -0.4 3,788,800 - +0.5 3,788,882 + RR42_RS37105 0.19 -0.1 3,788,896 + RR42_RS37105 0.24 -0.2 3,788,897 + RR42_RS37105 0.25 -0.8 3,788,898 - RR42_RS37105 0.25 +1.2 3,788,898 - RR42_RS37105 0.25 -1.2 3,788,898 - RR42_RS37105 0.25 +1.1 3,788,900 + RR42_RS37105 0.26 -1.6 3,788,901 - RR42_RS37105 0.26 +0.5 3,788,901 - RR42_RS37105 0.26 -1.4 3,788,901 - RR42_RS37105 0.26 -0.4 3,788,901 - RR42_RS37105 0.26 +0.3 3,788,909 + RR42_RS37105 0.30 -1.2 3,788,909 + RR42_RS37105 0.30 +0.2 3,788,910 - RR42_RS37105 0.30 +1.2 3,788,910 - RR42_RS37105 0.30 -1.9 3,788,910 - RR42_RS37105 0.30 -0.3 3,788,910 - RR42_RS37105 0.30 +0.1 3,788,910 - RR42_RS37105 0.30 -0.2 3,788,912 + RR42_RS37105 0.31 +0.2 3,788,912 + RR42_RS37105 0.31 +0.3 3,788,913 - RR42_RS37105 0.31 +1.5 3,788,955 + RR42_RS37105 0.48 -1.6 3,788,955 + RR42_RS37105 0.48 -0.1 3,788,956 - RR42_RS37105 0.49 +0.2
Or see this region's nucleotide sequence