Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS36825 and RR42_RS36830 are separated by 7 nucleotides RR42_RS36830 and RR42_RS36835 are separated by 57 nucleotides RR42_RS36835 and RR42_RS36840 are separated by 242 nucleotides
RR42_RS36825: RR42_RS36825 - membrane protein, at 3,714,529 to 3,715,284
_RS36825
RR42_RS36830: RR42_RS36830 - antibiotic biosynthesis monooxygenase, at 3,715,292 to 3,715,666
_RS36830
RR42_RS36835: RR42_RS36835 - cytochrome P450, at 3,715,724 to 3,716,848
_RS36835
RR42_RS36840: RR42_RS36840 - hydrolase, at 3,717,091 to 3,717,780
_RS36840
Position (kb)
3715
3716
3717 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3714.807 kb on + strand, within RR42_RS36825 at 3714.808 kb on - strand, within RR42_RS36825 at 3714.809 kb on + strand, within RR42_RS36825 at 3714.809 kb on + strand, within RR42_RS36825 at 3714.809 kb on + strand, within RR42_RS36825 at 3714.810 kb on - strand, within RR42_RS36825 at 3714.810 kb on - strand, within RR42_RS36825 at 3714.810 kb on - strand, within RR42_RS36825 at 3714.944 kb on + strand, within RR42_RS36825 at 3714.944 kb on + strand, within RR42_RS36825 at 3714.945 kb on - strand, within RR42_RS36825 at 3714.945 kb on - strand, within RR42_RS36825 at 3714.945 kb on - strand, within RR42_RS36825 at 3715.111 kb on + strand, within RR42_RS36825 at 3715.188 kb on + strand, within RR42_RS36825 at 3715.239 kb on + strand at 3715.377 kb on + strand, within RR42_RS36830 at 3715.561 kb on + strand, within RR42_RS36830 at 3715.562 kb on - strand, within RR42_RS36830 at 3715.562 kb on - strand, within RR42_RS36830 at 3715.614 kb on + strand, within RR42_RS36830 at 3715.614 kb on + strand, within RR42_RS36830 at 3715.614 kb on + strand, within RR42_RS36830 at 3715.614 kb on + strand, within RR42_RS36830 at 3715.614 kb on + strand, within RR42_RS36830 at 3715.614 kb on + strand, within RR42_RS36830 at 3715.614 kb on + strand, within RR42_RS36830 at 3715.614 kb on + strand, within RR42_RS36830 at 3715.615 kb on - strand, within RR42_RS36830 at 3715.615 kb on - strand, within RR42_RS36830 at 3715.615 kb on - strand, within RR42_RS36830 at 3715.615 kb on - strand, within RR42_RS36830 at 3715.615 kb on - strand, within RR42_RS36830 at 3716.092 kb on + strand, within RR42_RS36835 at 3716.349 kb on - strand, within RR42_RS36835 at 3716.742 kb on + strand at 3716.773 kb on - strand at 3716.775 kb on + strand at 3716.775 kb on + strand at 3716.776 kb on - strand at 3717.050 kb on + strand at 3717.050 kb on + strand at 3717.051 kb on - strand at 3717.051 kb on - strand at 3717.093 kb on - strand at 3717.137 kb on + strand at 3717.137 kb on + strand at 3717.137 kb on + strand at 3717.138 kb on - strand at 3717.179 kb on + strand, within RR42_RS36840 at 3717.179 kb on + strand, within RR42_RS36840 at 3717.179 kb on + strand, within RR42_RS36840 at 3717.180 kb on - strand, within RR42_RS36840 at 3717.425 kb on + strand, within RR42_RS36840 at 3717.425 kb on + strand, within RR42_RS36840 at 3717.512 kb on + strand, within RR42_RS36840 at 3717.512 kb on + strand, within RR42_RS36840 at 3717.512 kb on + strand, within RR42_RS36840 at 3717.515 kb on + strand, within RR42_RS36840 at 3717.515 kb on + strand, within RR42_RS36840 at 3717.515 kb on + strand, within RR42_RS36840 at 3717.515 kb on + strand, within RR42_RS36840 at 3717.515 kb on + strand, within RR42_RS36840 at 3717.516 kb on - strand, within RR42_RS36840 at 3717.630 kb on + strand, within RR42_RS36840 at 3717.630 kb on + strand, within RR42_RS36840 at 3717.630 kb on + strand, within RR42_RS36840 at 3717.631 kb on - strand, within RR42_RS36840 at 3717.771 kb on + strand at 3717.771 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 3,714,807 + RR42_RS36825 0.37 +0.5 3,714,808 - RR42_RS36825 0.37 -0.0 3,714,809 + RR42_RS36825 0.37 -0.4 3,714,809 + RR42_RS36825 0.37 +1.8 3,714,809 + RR42_RS36825 0.37 -0.4 3,714,810 - RR42_RS36825 0.37 -2.6 3,714,810 - RR42_RS36825 0.37 +0.3 3,714,810 - RR42_RS36825 0.37 +0.2 3,714,944 + RR42_RS36825 0.55 +0.4 3,714,944 + RR42_RS36825 0.55 -0.6 3,714,945 - RR42_RS36825 0.55 -0.1 3,714,945 - RR42_RS36825 0.55 +0.0 3,714,945 - RR42_RS36825 0.55 +0.4 3,715,111 + RR42_RS36825 0.77 -0.5 3,715,188 + RR42_RS36825 0.87 -1.3 3,715,239 + +0.3 3,715,377 + RR42_RS36830 0.23 -1.5 3,715,561 + RR42_RS36830 0.72 -1.4 3,715,562 - RR42_RS36830 0.72 +0.5 3,715,562 - RR42_RS36830 0.72 -1.7 3,715,614 + RR42_RS36830 0.86 -0.7 3,715,614 + RR42_RS36830 0.86 -0.2 3,715,614 + RR42_RS36830 0.86 +0.9 3,715,614 + RR42_RS36830 0.86 -0.3 3,715,614 + RR42_RS36830 0.86 -0.7 3,715,614 + RR42_RS36830 0.86 -0.5 3,715,614 + RR42_RS36830 0.86 -1.7 3,715,614 + RR42_RS36830 0.86 +0.2 3,715,615 - RR42_RS36830 0.86 -1.3 3,715,615 - RR42_RS36830 0.86 -1.1 3,715,615 - RR42_RS36830 0.86 -0.2 3,715,615 - RR42_RS36830 0.86 +2.1 3,715,615 - RR42_RS36830 0.86 -2.0 3,716,092 + RR42_RS36835 0.33 +0.8 3,716,349 - RR42_RS36835 0.56 +0.3 3,716,742 + +0.1 3,716,773 - -1.2 3,716,775 + +0.3 3,716,775 + -0.2 3,716,776 - +0.0 3,717,050 + +0.3 3,717,050 + -0.2 3,717,051 - +1.4 3,717,051 - +0.1 3,717,093 - +1.0 3,717,137 + -0.9 3,717,137 + +0.4 3,717,137 + +0.4 3,717,138 - +1.4 3,717,179 + RR42_RS36840 0.13 +0.1 3,717,179 + RR42_RS36840 0.13 +0.5 3,717,179 + RR42_RS36840 0.13 -0.5 3,717,180 - RR42_RS36840 0.13 +0.3 3,717,425 + RR42_RS36840 0.48 +0.4 3,717,425 + RR42_RS36840 0.48 +0.2 3,717,512 + RR42_RS36840 0.61 +0.2 3,717,512 + RR42_RS36840 0.61 +0.4 3,717,512 + RR42_RS36840 0.61 +0.1 3,717,515 + RR42_RS36840 0.61 -0.3 3,717,515 + RR42_RS36840 0.61 +0.6 3,717,515 + RR42_RS36840 0.61 -1.5 3,717,515 + RR42_RS36840 0.61 -0.5 3,717,515 + RR42_RS36840 0.61 +0.4 3,717,516 - RR42_RS36840 0.62 -0.5 3,717,630 + RR42_RS36840 0.78 +0.4 3,717,630 + RR42_RS36840 0.78 +0.1 3,717,630 + RR42_RS36840 0.78 -0.1 3,717,631 - RR42_RS36840 0.78 -1.6 3,717,771 + -0.5 3,717,771 + -0.8
Or see this region's nucleotide sequence