Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS34675 and RR42_RS34680 are separated by 58 nucleotides RR42_RS34680 and RR42_RS34685 are separated by 75 nucleotides RR42_RS34685 and RR42_RS34690 are separated by 210 nucleotides
RR42_RS34675: RR42_RS34675 - NADPH:quinone reductase, at 3,220,094 to 3,220,978
_RS34675
RR42_RS34680: RR42_RS34680 - short-chain dehydrogenase, at 3,221,037 to 3,221,753
_RS34680
RR42_RS34685: RR42_RS34685 - catechol 1,2-dioxygenase, at 3,221,829 to 3,222,695
_RS34685
RR42_RS34690: RR42_RS34690 - permease, at 3,222,906 to 3,223,622
_RS34690
Position (kb)
3221
3222
3223 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3220.954 kb on + strand at 3220.955 kb on - strand at 3220.972 kb on + strand at 3221.233 kb on - strand, within RR42_RS34680 at 3221.546 kb on - strand, within RR42_RS34680 at 3221.826 kb on + strand at 3221.897 kb on - strand at 3222.043 kb on + strand, within RR42_RS34685 at 3222.085 kb on + strand, within RR42_RS34685 at 3222.146 kb on - strand, within RR42_RS34685 at 3222.248 kb on - strand, within RR42_RS34685 at 3222.386 kb on - strand, within RR42_RS34685 at 3222.406 kb on + strand, within RR42_RS34685 at 3222.662 kb on + strand at 3222.663 kb on - strand at 3222.856 kb on - strand at 3222.856 kb on - strand at 3222.856 kb on - strand at 3222.862 kb on + strand at 3222.862 kb on + strand at 3222.862 kb on + strand at 3222.862 kb on + strand at 3222.863 kb on - strand at 3223.011 kb on + strand, within RR42_RS34690 at 3223.545 kb on + strand, within RR42_RS34690 at 3223.545 kb on + strand, within RR42_RS34690 at 3223.546 kb on - strand, within RR42_RS34690 at 3223.551 kb on + strand at 3223.551 kb on + strand at 3223.551 kb on + strand at 3223.551 kb on + strand at 3223.552 kb on - strand at 3223.566 kb on + strand at 3223.611 kb on + strand at 3223.612 kb on - strand at 3223.613 kb on + strand at 3223.613 kb on + strand at 3223.613 kb on + strand at 3223.613 kb on + strand at 3223.613 kb on + strand at 3223.613 kb on + strand at 3223.614 kb on - strand at 3223.614 kb on - strand at 3223.614 kb on - strand at 3223.614 kb on - strand at 3223.614 kb on - strand at 3223.614 kb on - strand at 3223.614 kb on - strand at 3223.614 kb on - strand at 3223.614 kb on - strand at 3223.614 kb on - strand at 3223.618 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 3,220,954 + +0.4 3,220,955 - +0.4 3,220,972 + -0.3 3,221,233 - RR42_RS34680 0.27 -1.3 3,221,546 - RR42_RS34680 0.71 +0.3 3,221,826 + -0.2 3,221,897 - +0.6 3,222,043 + RR42_RS34685 0.25 -1.0 3,222,085 + RR42_RS34685 0.30 -1.2 3,222,146 - RR42_RS34685 0.37 -1.3 3,222,248 - RR42_RS34685 0.48 -0.4 3,222,386 - RR42_RS34685 0.64 -0.9 3,222,406 + RR42_RS34685 0.67 +0.2 3,222,662 + +0.8 3,222,663 - +0.1 3,222,856 - -0.4 3,222,856 - -0.7 3,222,856 - +0.2 3,222,862 + -1.0 3,222,862 + -0.7 3,222,862 + -0.1 3,222,862 + -0.4 3,222,863 - -0.2 3,223,011 + RR42_RS34690 0.15 +0.2 3,223,545 + RR42_RS34690 0.89 +0.4 3,223,545 + RR42_RS34690 0.89 -0.3 3,223,546 - RR42_RS34690 0.89 -0.6 3,223,551 + -0.5 3,223,551 + +1.0 3,223,551 + -0.1 3,223,551 + -1.1 3,223,552 - +1.0 3,223,566 + +0.2 3,223,611 + -1.8 3,223,612 - +2.3 3,223,613 + -1.2 3,223,613 + +0.5 3,223,613 + -1.3 3,223,613 + -0.7 3,223,613 + +1.1 3,223,613 + -0.3 3,223,614 - -3.0 3,223,614 - -0.8 3,223,614 - +0.3 3,223,614 - -1.4 3,223,614 - -0.7 3,223,614 - +0.8 3,223,614 - -0.5 3,223,614 - -0.1 3,223,614 - -1.3 3,223,614 - -0.0 3,223,618 - -0.7
Or see this region's nucleotide sequence