Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32440 and RR42_RS32445 are separated by 525 nucleotides RR42_RS32445 and RR42_RS32450 are separated by 33 nucleotides RR42_RS32450 and RR42_RS32455 are separated by 77 nucleotides
RR42_RS32440: RR42_RS32440 - 4-hydroxyphenylpyruvate dioxygenase, at 2,675,093 to 2,676,130
_RS32440
RR42_RS32445: RR42_RS32445 - shikimate dehydrogenase, at 2,676,656 to 2,677,489
_RS32445
RR42_RS32450: RR42_RS32450 - 3-dehydroquinate dehydratase, at 2,677,523 to 2,677,975
_RS32450
RR42_RS32455: RR42_RS32455 - oxidoreductase, at 2,678,053 to 2,679,105
_RS32455
Position (kb)
2676
2677
2678 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2675.668 kb on + strand, within RR42_RS32440 at 2675.721 kb on + strand, within RR42_RS32440 at 2675.721 kb on + strand, within RR42_RS32440 at 2675.721 kb on + strand, within RR42_RS32440 at 2675.722 kb on - strand, within RR42_RS32440 at 2675.722 kb on - strand, within RR42_RS32440 at 2675.722 kb on - strand, within RR42_RS32440 at 2675.722 kb on - strand, within RR42_RS32440 at 2675.779 kb on + strand, within RR42_RS32440 at 2675.784 kb on + strand, within RR42_RS32440 at 2675.784 kb on + strand, within RR42_RS32440 at 2675.785 kb on - strand, within RR42_RS32440 at 2675.785 kb on - strand, within RR42_RS32440 at 2675.943 kb on + strand, within RR42_RS32440 at 2675.955 kb on + strand, within RR42_RS32440 at 2675.956 kb on - strand, within RR42_RS32440 at 2675.956 kb on - strand, within RR42_RS32440 at 2676.123 kb on + strand at 2676.217 kb on - strand at 2676.253 kb on - strand at 2676.253 kb on - strand at 2676.253 kb on - strand at 2676.253 kb on - strand at 2676.253 kb on - strand at 2676.384 kb on + strand at 2676.425 kb on - strand at 2676.433 kb on - strand at 2676.507 kb on + strand at 2676.508 kb on - strand at 2676.568 kb on - strand at 2676.626 kb on + strand at 2676.633 kb on + strand at 2676.633 kb on + strand at 2676.633 kb on + strand at 2676.633 kb on + strand at 2676.634 kb on - strand at 2676.634 kb on - strand at 2676.634 kb on - strand at 2676.728 kb on + strand at 2676.728 kb on + strand at 2676.728 kb on + strand at 2676.728 kb on + strand at 2676.728 kb on + strand at 2676.728 kb on + strand at 2676.729 kb on - strand at 2676.729 kb on - strand at 2676.729 kb on - strand at 2676.729 kb on - strand at 2676.898 kb on + strand, within RR42_RS32445 at 2677.334 kb on + strand, within RR42_RS32445 at 2677.335 kb on - strand, within RR42_RS32445 at 2677.419 kb on - strand at 2677.419 kb on - strand at 2677.630 kb on - strand, within RR42_RS32450 at 2677.630 kb on - strand, within RR42_RS32450 at 2677.630 kb on - strand, within RR42_RS32450 at 2677.630 kb on - strand, within RR42_RS32450 at 2677.672 kb on + strand, within RR42_RS32450 at 2677.673 kb on - strand, within RR42_RS32450 at 2677.673 kb on - strand, within RR42_RS32450 at 2677.673 kb on - strand, within RR42_RS32450 at 2677.673 kb on - strand, within RR42_RS32450 at 2677.908 kb on + strand, within RR42_RS32450 at 2677.909 kb on - strand, within RR42_RS32450 at 2677.909 kb on - strand, within RR42_RS32450 at 2677.965 kb on - strand at 2678.279 kb on + strand, within RR42_RS32455 at 2678.279 kb on + strand, within RR42_RS32455 at 2678.279 kb on + strand, within RR42_RS32455 at 2678.280 kb on - strand, within RR42_RS32455 at 2678.336 kb on + strand, within RR42_RS32455 at 2678.337 kb on - strand, within RR42_RS32455 at 2678.337 kb on - strand, within RR42_RS32455 at 2678.363 kb on + strand, within RR42_RS32455
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 2,675,668 + RR42_RS32440 0.55 -0.4 2,675,721 + RR42_RS32440 0.61 +0.2 2,675,721 + RR42_RS32440 0.61 +0.6 2,675,721 + RR42_RS32440 0.61 -1.0 2,675,722 - RR42_RS32440 0.61 -0.1 2,675,722 - RR42_RS32440 0.61 -0.4 2,675,722 - RR42_RS32440 0.61 -0.9 2,675,722 - RR42_RS32440 0.61 -0.7 2,675,779 + RR42_RS32440 0.66 -0.6 2,675,784 + RR42_RS32440 0.67 +0.7 2,675,784 + RR42_RS32440 0.67 -0.4 2,675,785 - RR42_RS32440 0.67 +0.2 2,675,785 - RR42_RS32440 0.67 -0.5 2,675,943 + RR42_RS32440 0.82 -1.8 2,675,955 + RR42_RS32440 0.83 -1.2 2,675,956 - RR42_RS32440 0.83 +1.5 2,675,956 - RR42_RS32440 0.83 +0.1 2,676,123 + +0.0 2,676,217 - +0.3 2,676,253 - -1.9 2,676,253 - -0.1 2,676,253 - +0.4 2,676,253 - +1.4 2,676,253 - -0.3 2,676,384 + +2.4 2,676,425 - +0.6 2,676,433 - -0.5 2,676,507 + +0.4 2,676,508 - -0.4 2,676,568 - -0.7 2,676,626 + +1.0 2,676,633 + -0.3 2,676,633 + +0.3 2,676,633 + -0.4 2,676,633 + -0.4 2,676,634 - +0.4 2,676,634 - +1.3 2,676,634 - -0.2 2,676,728 + -0.0 2,676,728 + -0.7 2,676,728 + -0.4 2,676,728 + -0.1 2,676,728 + +0.2 2,676,728 + +1.9 2,676,729 - -0.1 2,676,729 - -0.4 2,676,729 - -0.8 2,676,729 - +0.7 2,676,898 + RR42_RS32445 0.29 +0.3 2,677,334 + RR42_RS32445 0.81 +1.2 2,677,335 - RR42_RS32445 0.81 +0.0 2,677,419 - +1.1 2,677,419 - +0.3 2,677,630 - RR42_RS32450 0.24 -0.1 2,677,630 - RR42_RS32450 0.24 -0.2 2,677,630 - RR42_RS32450 0.24 -0.1 2,677,630 - RR42_RS32450 0.24 -1.2 2,677,672 + RR42_RS32450 0.33 +0.6 2,677,673 - RR42_RS32450 0.33 -1.4 2,677,673 - RR42_RS32450 0.33 +0.3 2,677,673 - RR42_RS32450 0.33 +0.3 2,677,673 - RR42_RS32450 0.33 +0.7 2,677,908 + RR42_RS32450 0.85 -1.1 2,677,909 - RR42_RS32450 0.85 +0.9 2,677,909 - RR42_RS32450 0.85 +0.8 2,677,965 - +0.1 2,678,279 + RR42_RS32455 0.21 -1.6 2,678,279 + RR42_RS32455 0.21 +0.3 2,678,279 + RR42_RS32455 0.21 -0.9 2,678,280 - RR42_RS32455 0.22 -0.4 2,678,336 + RR42_RS32455 0.27 -0.0 2,678,337 - RR42_RS32455 0.27 -0.7 2,678,337 - RR42_RS32455 0.27 +0.3 2,678,363 + RR42_RS32455 0.29 -0.4
Or see this region's nucleotide sequence