Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31815 and RR42_RS31820 are separated by 51 nucleotides RR42_RS31820 and RR42_RS31825 are separated by 56 nucleotides RR42_RS31825 and RR42_RS31830 are separated by 150 nucleotides RR42_RS31830 and RR42_RS31835 are separated by 13 nucleotides
RR42_RS31815: RR42_RS31815 - steroid delta-isomerase, at 2,515,564 to 2,515,953
_RS31815
RR42_RS31820: RR42_RS31820 - short-chain dehydrogenase, at 2,516,005 to 2,516,790
_RS31820
RR42_RS31825: RR42_RS31825 - 3-oxoacyl-ACP reductase, at 2,516,847 to 2,517,695
_RS31825
RR42_RS31830: RR42_RS31830 - short-chain dehydrogenase, at 2,517,846 to 2,518,622
_RS31830
RR42_RS31835: RR42_RS31835 - short-chain dehydrogenase, at 2,518,636 to 2,519,502
_RS31835
Position (kb)
2516
2517
2518 Strain fitness (log2 ratio)
-2
-1
0
1 at 2516.079 kb on - strand at 2516.208 kb on + strand, within RR42_RS31820 at 2516.209 kb on - strand, within RR42_RS31820 at 2516.253 kb on - strand, within RR42_RS31820 at 2516.339 kb on + strand, within RR42_RS31820 at 2516.784 kb on - strand at 2516.902 kb on + strand at 2516.902 kb on + strand at 2517.212 kb on + strand, within RR42_RS31825 at 2517.213 kb on - strand, within RR42_RS31825 at 2517.341 kb on - strand, within RR42_RS31825 at 2517.554 kb on + strand, within RR42_RS31825 at 2517.554 kb on + strand, within RR42_RS31825 at 2517.686 kb on + strand at 2517.877 kb on + strand at 2518.426 kb on - strand, within RR42_RS31830 at 2518.540 kb on - strand, within RR42_RS31830 at 2518.544 kb on - strand, within RR42_RS31830
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 2,516,079 - +1.4 2,516,208 + RR42_RS31820 0.26 -1.0 2,516,209 - RR42_RS31820 0.26 -2.4 2,516,253 - RR42_RS31820 0.32 -0.8 2,516,339 + RR42_RS31820 0.42 -1.1 2,516,784 - -0.7 2,516,902 + +1.6 2,516,902 + +0.7 2,517,212 + RR42_RS31825 0.43 -0.6 2,517,213 - RR42_RS31825 0.43 +0.3 2,517,341 - RR42_RS31825 0.58 +1.3 2,517,554 + RR42_RS31825 0.83 -0.3 2,517,554 + RR42_RS31825 0.83 +0.3 2,517,686 + -1.3 2,517,877 + +0.7 2,518,426 - RR42_RS31830 0.75 +0.3 2,518,540 - RR42_RS31830 0.89 +0.4 2,518,544 - RR42_RS31830 0.90 +1.2
Or see this region's nucleotide sequence