Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28965 and RR42_RS28970 are separated by 61 nucleotides RR42_RS28970 and RR42_RS28975 are separated by 15 nucleotides RR42_RS28975 and RR42_RS28980 are separated by 180 nucleotides
RR42_RS28965: RR42_RS28965 - hypothetical protein, at 1,856,240 to 1,856,707
_RS28965
RR42_RS28970: RR42_RS28970 - 3-oxoacyl-ACP reductase, at 1,856,769 to 1,857,527
_RS28970
RR42_RS28975: RR42_RS28975 - acid phosphatase, at 1,857,543 to 1,858,343
_RS28975
RR42_RS28980: RR42_RS28980 - fatty acid--CoA ligase, at 1,858,524 to 1,860,266
_RS28980
Position (kb)
1857
1858
1859 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1856.602 kb on + strand, within RR42_RS28965 at 1856.602 kb on + strand, within RR42_RS28965 at 1856.603 kb on - strand at 1856.603 kb on - strand, within RR42_RS28965 at 1856.613 kb on - strand, within RR42_RS28965 at 1856.621 kb on + strand, within RR42_RS28965 at 1856.621 kb on + strand, within RR42_RS28965 at 1856.622 kb on - strand, within RR42_RS28965 at 1856.622 kb on - strand, within RR42_RS28965 at 1856.656 kb on + strand, within RR42_RS28965 at 1856.705 kb on + strand at 1856.705 kb on + strand at 1856.705 kb on + strand at 1856.705 kb on + strand at 1856.705 kb on + strand at 1856.705 kb on + strand at 1856.705 kb on + strand at 1856.706 kb on - strand at 1856.706 kb on - strand at 1856.706 kb on - strand at 1856.879 kb on + strand, within RR42_RS28970 at 1857.237 kb on + strand, within RR42_RS28970 at 1857.238 kb on - strand, within RR42_RS28970 at 1857.238 kb on - strand, within RR42_RS28970 at 1857.238 kb on - strand, within RR42_RS28970 at 1857.325 kb on - strand, within RR42_RS28970 at 1857.348 kb on - strand, within RR42_RS28970 at 1857.544 kb on + strand at 1857.544 kb on + strand at 1857.544 kb on + strand at 1857.545 kb on - strand at 1857.757 kb on + strand, within RR42_RS28975 at 1857.757 kb on + strand, within RR42_RS28975 at 1857.758 kb on - strand, within RR42_RS28975 at 1857.758 kb on - strand, within RR42_RS28975 at 1857.842 kb on + strand, within RR42_RS28975 at 1857.857 kb on - strand at 1857.940 kb on + strand, within RR42_RS28975 at 1857.940 kb on + strand, within RR42_RS28975 at 1857.941 kb on - strand, within RR42_RS28975 at 1857.941 kb on - strand, within RR42_RS28975 at 1857.967 kb on - strand, within RR42_RS28975 at 1858.004 kb on - strand, within RR42_RS28975 at 1858.260 kb on + strand, within RR42_RS28975 at 1858.774 kb on + strand, within RR42_RS28980 at 1858.801 kb on + strand, within RR42_RS28980 at 1858.801 kb on + strand, within RR42_RS28980 at 1858.801 kb on + strand, within RR42_RS28980 at 1858.801 kb on + strand, within RR42_RS28980 at 1858.801 kb on + strand, within RR42_RS28980 at 1858.802 kb on - strand, within RR42_RS28980 at 1858.802 kb on - strand, within RR42_RS28980 at 1858.802 kb on - strand, within RR42_RS28980 at 1858.829 kb on + strand, within RR42_RS28980 at 1858.829 kb on + strand, within RR42_RS28980 at 1858.829 kb on + strand, within RR42_RS28980 at 1858.850 kb on + strand, within RR42_RS28980 at 1858.850 kb on + strand, within RR42_RS28980 at 1858.851 kb on - strand, within RR42_RS28980 at 1858.851 kb on - strand, within RR42_RS28980 at 1858.851 kb on - strand, within RR42_RS28980 at 1858.852 kb on + strand, within RR42_RS28980 at 1858.852 kb on + strand, within RR42_RS28980 at 1858.852 kb on + strand, within RR42_RS28980 at 1858.852 kb on + strand, within RR42_RS28980 at 1858.852 kb on + strand, within RR42_RS28980 at 1858.852 kb on + strand, within RR42_RS28980 at 1858.852 kb on + strand, within RR42_RS28980 at 1858.853 kb on - strand, within RR42_RS28980 at 1858.853 kb on - strand, within RR42_RS28980 at 1858.853 kb on - strand, within RR42_RS28980 at 1858.853 kb on - strand, within RR42_RS28980 at 1859.080 kb on + strand, within RR42_RS28980 at 1859.080 kb on + strand, within RR42_RS28980 at 1859.080 kb on + strand, within RR42_RS28980 at 1859.081 kb on - strand, within RR42_RS28980 at 1859.081 kb on - strand, within RR42_RS28980
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 1,856,602 + RR42_RS28965 0.77 +0.5 1,856,602 + RR42_RS28965 0.77 -1.2 1,856,603 - +0.2 1,856,603 - RR42_RS28965 0.78 -0.0 1,856,613 - RR42_RS28965 0.80 -1.1 1,856,621 + RR42_RS28965 0.81 -1.0 1,856,621 + RR42_RS28965 0.81 +1.5 1,856,622 - RR42_RS28965 0.82 -0.4 1,856,622 - RR42_RS28965 0.82 -0.6 1,856,656 + RR42_RS28965 0.89 -0.0 1,856,705 + +0.2 1,856,705 + -0.3 1,856,705 + +0.2 1,856,705 + -0.2 1,856,705 + +2.2 1,856,705 + -0.0 1,856,705 + -0.7 1,856,706 - +0.1 1,856,706 - +1.2 1,856,706 - +2.6 1,856,879 + RR42_RS28970 0.14 -0.2 1,857,237 + RR42_RS28970 0.62 +0.4 1,857,238 - RR42_RS28970 0.62 -0.2 1,857,238 - RR42_RS28970 0.62 -0.3 1,857,238 - RR42_RS28970 0.62 -0.4 1,857,325 - RR42_RS28970 0.73 +0.1 1,857,348 - RR42_RS28970 0.76 -0.5 1,857,544 + -0.2 1,857,544 + +0.2 1,857,544 + +0.0 1,857,545 - -2.2 1,857,757 + RR42_RS28975 0.27 +0.3 1,857,757 + RR42_RS28975 0.27 +0.8 1,857,758 - RR42_RS28975 0.27 +0.1 1,857,758 - RR42_RS28975 0.27 -0.4 1,857,842 + RR42_RS28975 0.37 -0.5 1,857,857 - +2.2 1,857,940 + RR42_RS28975 0.50 +1.0 1,857,940 + RR42_RS28975 0.50 +0.7 1,857,941 - RR42_RS28975 0.50 +0.1 1,857,941 - RR42_RS28975 0.50 -0.9 1,857,967 - RR42_RS28975 0.53 +0.1 1,858,004 - RR42_RS28975 0.58 -0.8 1,858,260 + RR42_RS28975 0.90 -0.4 1,858,774 + RR42_RS28980 0.14 -1.3 1,858,801 + RR42_RS28980 0.16 -1.9 1,858,801 + RR42_RS28980 0.16 +0.5 1,858,801 + RR42_RS28980 0.16 -1.1 1,858,801 + RR42_RS28980 0.16 -0.3 1,858,801 + RR42_RS28980 0.16 -0.2 1,858,802 - RR42_RS28980 0.16 -0.5 1,858,802 - RR42_RS28980 0.16 -0.2 1,858,802 - RR42_RS28980 0.16 +1.4 1,858,829 + RR42_RS28980 0.17 -1.2 1,858,829 + RR42_RS28980 0.17 -0.5 1,858,829 + RR42_RS28980 0.17 -0.6 1,858,850 + RR42_RS28980 0.19 -0.1 1,858,850 + RR42_RS28980 0.19 +0.2 1,858,851 - RR42_RS28980 0.19 +0.1 1,858,851 - RR42_RS28980 0.19 +0.1 1,858,851 - RR42_RS28980 0.19 +0.8 1,858,852 + RR42_RS28980 0.19 +1.2 1,858,852 + RR42_RS28980 0.19 +0.5 1,858,852 + RR42_RS28980 0.19 -1.5 1,858,852 + RR42_RS28980 0.19 +0.3 1,858,852 + RR42_RS28980 0.19 -0.1 1,858,852 + RR42_RS28980 0.19 +0.4 1,858,852 + RR42_RS28980 0.19 -0.9 1,858,853 - RR42_RS28980 0.19 -0.3 1,858,853 - RR42_RS28980 0.19 +0.6 1,858,853 - RR42_RS28980 0.19 -0.3 1,858,853 - RR42_RS28980 0.19 +0.4 1,859,080 + RR42_RS28980 0.32 -0.0 1,859,080 + RR42_RS28980 0.32 +0.5 1,859,080 + RR42_RS28980 0.32 -0.0 1,859,081 - RR42_RS28980 0.32 -1.0 1,859,081 - RR42_RS28980 0.32 -0.6
Or see this region's nucleotide sequence