Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28930 and RR42_RS28935 are separated by 61 nucleotides RR42_RS28935 and RR42_RS28940 are separated by 396 nucleotides
RR42_RS28930: RR42_RS28930 - glucosyltransferase MdoH, at 1,847,462 to 1,850,014
_RS28930
RR42_RS28935: RR42_RS28935 - hypothetical protein, at 1,850,076 to 1,850,657
_RS28935
RR42_RS28940: RR42_RS28940 - Bcr/CflA family drug resistance efflux transporter, at 1,851,054 to 1,852,274
_RS28940
Position (kb)
1850
1851 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1849.128 kb on - strand, within RR42_RS28930 at 1849.143 kb on - strand, within RR42_RS28930 at 1849.271 kb on + strand, within RR42_RS28930 at 1849.272 kb on - strand, within RR42_RS28930 at 1849.272 kb on - strand, within RR42_RS28930 at 1849.396 kb on + strand, within RR42_RS28930 at 1849.397 kb on - strand, within RR42_RS28930 at 1849.397 kb on - strand, within RR42_RS28930 at 1849.792 kb on + strand at 1849.917 kb on + strand at 1850.077 kb on + strand at 1850.113 kb on + strand at 1850.114 kb on - strand at 1850.114 kb on - strand at 1850.114 kb on - strand at 1850.158 kb on + strand, within RR42_RS28935 at 1850.158 kb on + strand, within RR42_RS28935 at 1850.159 kb on - strand, within RR42_RS28935 at 1850.159 kb on - strand, within RR42_RS28935 at 1850.219 kb on + strand, within RR42_RS28935 at 1850.242 kb on + strand, within RR42_RS28935 at 1850.312 kb on + strand, within RR42_RS28935 at 1850.312 kb on + strand, within RR42_RS28935 at 1850.313 kb on - strand, within RR42_RS28935 at 1850.313 kb on - strand, within RR42_RS28935 at 1850.409 kb on + strand, within RR42_RS28935 at 1850.409 kb on + strand, within RR42_RS28935 at 1850.409 kb on + strand, within RR42_RS28935 at 1850.409 kb on + strand, within RR42_RS28935 at 1850.409 kb on + strand, within RR42_RS28935 at 1850.410 kb on - strand, within RR42_RS28935 at 1850.410 kb on - strand, within RR42_RS28935 at 1850.410 kb on - strand, within RR42_RS28935 at 1850.410 kb on - strand, within RR42_RS28935 at 1850.410 kb on - strand, within RR42_RS28935 at 1850.410 kb on - strand, within RR42_RS28935 at 1850.430 kb on - strand, within RR42_RS28935 at 1850.465 kb on + strand, within RR42_RS28935 at 1850.466 kb on - strand at 1850.600 kb on + strand at 1850.644 kb on + strand at 1850.644 kb on + strand at 1850.644 kb on + strand at 1850.645 kb on - strand at 1850.645 kb on - strand at 1850.645 kb on - strand at 1850.645 kb on - strand at 1850.757 kb on - strand at 1850.937 kb on + strand at 1850.953 kb on + strand at 1850.954 kb on - strand at 1850.962 kb on - strand at 1851.110 kb on - strand at 1851.110 kb on - strand at 1851.153 kb on + strand at 1851.153 kb on + strand at 1851.153 kb on + strand at 1851.153 kb on + strand at 1851.154 kb on - strand at 1851.154 kb on - strand at 1851.261 kb on + strand, within RR42_RS28940 at 1851.261 kb on + strand, within RR42_RS28940 at 1851.261 kb on + strand, within RR42_RS28940 at 1851.261 kb on + strand, within RR42_RS28940 at 1851.261 kb on + strand, within RR42_RS28940 at 1851.262 kb on - strand, within RR42_RS28940 at 1851.262 kb on - strand, within RR42_RS28940 at 1851.262 kb on - strand, within RR42_RS28940 at 1851.262 kb on - strand, within RR42_RS28940 at 1851.303 kb on + strand, within RR42_RS28940 at 1851.303 kb on + strand, within RR42_RS28940 at 1851.304 kb on - strand, within RR42_RS28940 at 1851.304 kb on - strand, within RR42_RS28940 at 1851.304 kb on - strand, within RR42_RS28940 at 1851.304 kb on - strand, within RR42_RS28940 at 1851.321 kb on + strand, within RR42_RS28940 at 1851.321 kb on + strand, within RR42_RS28940 at 1851.321 kb on + strand, within RR42_RS28940 at 1851.321 kb on + strand, within RR42_RS28940 at 1851.618 kb on + strand, within RR42_RS28940 at 1851.618 kb on + strand, within RR42_RS28940 at 1851.618 kb on + strand, within RR42_RS28940 at 1851.618 kb on + strand, within RR42_RS28940 at 1851.619 kb on - strand, within RR42_RS28940 at 1851.619 kb on - strand, within RR42_RS28940 at 1851.620 kb on + strand, within RR42_RS28940 at 1851.620 kb on + strand, within RR42_RS28940 at 1851.621 kb on - strand, within RR42_RS28940
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 1,849,128 - RR42_RS28930 0.65 +0.3 1,849,143 - RR42_RS28930 0.66 +0.6 1,849,271 + RR42_RS28930 0.71 -0.3 1,849,272 - RR42_RS28930 0.71 +0.8 1,849,272 - RR42_RS28930 0.71 +0.0 1,849,396 + RR42_RS28930 0.76 -0.9 1,849,397 - RR42_RS28930 0.76 +0.5 1,849,397 - RR42_RS28930 0.76 -0.8 1,849,792 + -0.9 1,849,917 + -0.1 1,850,077 + -0.6 1,850,113 + -0.3 1,850,114 - -0.2 1,850,114 - -0.3 1,850,114 - -1.1 1,850,158 + RR42_RS28935 0.14 -0.2 1,850,158 + RR42_RS28935 0.14 -0.5 1,850,159 - RR42_RS28935 0.14 -0.6 1,850,159 - RR42_RS28935 0.14 -0.1 1,850,219 + RR42_RS28935 0.25 -1.1 1,850,242 + RR42_RS28935 0.29 -0.6 1,850,312 + RR42_RS28935 0.41 -1.0 1,850,312 + RR42_RS28935 0.41 -1.5 1,850,313 - RR42_RS28935 0.41 -0.0 1,850,313 - RR42_RS28935 0.41 -1.8 1,850,409 + RR42_RS28935 0.57 +0.2 1,850,409 + RR42_RS28935 0.57 +0.2 1,850,409 + RR42_RS28935 0.57 -0.7 1,850,409 + RR42_RS28935 0.57 +0.2 1,850,409 + RR42_RS28935 0.57 -0.6 1,850,410 - RR42_RS28935 0.57 -0.6 1,850,410 - RR42_RS28935 0.57 -0.7 1,850,410 - RR42_RS28935 0.57 -0.4 1,850,410 - RR42_RS28935 0.57 -0.5 1,850,410 - RR42_RS28935 0.57 +0.3 1,850,410 - RR42_RS28935 0.57 -0.8 1,850,430 - RR42_RS28935 0.61 +0.2 1,850,465 + RR42_RS28935 0.67 +1.6 1,850,466 - +1.2 1,850,600 + +0.3 1,850,644 + +2.2 1,850,644 + -0.3 1,850,644 + -0.2 1,850,645 - -0.7 1,850,645 - -0.5 1,850,645 - -0.5 1,850,645 - -0.6 1,850,757 - -0.7 1,850,937 + -1.7 1,850,953 + -2.3 1,850,954 - -1.5 1,850,962 - +0.6 1,851,110 - +0.5 1,851,110 - -0.0 1,851,153 + +0.4 1,851,153 + -0.2 1,851,153 + +1.2 1,851,153 + -0.0 1,851,154 - -0.3 1,851,154 - +0.3 1,851,261 + RR42_RS28940 0.17 -0.1 1,851,261 + RR42_RS28940 0.17 -0.7 1,851,261 + RR42_RS28940 0.17 -0.2 1,851,261 + RR42_RS28940 0.17 -0.9 1,851,261 + RR42_RS28940 0.17 -0.1 1,851,262 - RR42_RS28940 0.17 +0.6 1,851,262 - RR42_RS28940 0.17 -0.6 1,851,262 - RR42_RS28940 0.17 -0.1 1,851,262 - RR42_RS28940 0.17 -0.3 1,851,303 + RR42_RS28940 0.20 +0.8 1,851,303 + RR42_RS28940 0.20 -0.6 1,851,304 - RR42_RS28940 0.20 -1.1 1,851,304 - RR42_RS28940 0.20 -0.1 1,851,304 - RR42_RS28940 0.20 +0.2 1,851,304 - RR42_RS28940 0.20 -0.8 1,851,321 + RR42_RS28940 0.22 -0.3 1,851,321 + RR42_RS28940 0.22 -0.3 1,851,321 + RR42_RS28940 0.22 +0.1 1,851,321 + RR42_RS28940 0.22 +0.4 1,851,618 + RR42_RS28940 0.46 +1.6 1,851,618 + RR42_RS28940 0.46 -0.8 1,851,618 + RR42_RS28940 0.46 +1.1 1,851,618 + RR42_RS28940 0.46 -0.4 1,851,619 - RR42_RS28940 0.46 +0.3 1,851,619 - RR42_RS28940 0.46 +0.8 1,851,620 + RR42_RS28940 0.46 -0.6 1,851,620 + RR42_RS28940 0.46 +0.3 1,851,621 - RR42_RS28940 0.46 -0.7
Or see this region's nucleotide sequence