Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28390 and RR42_RS28395 overlap by 4 nucleotides RR42_RS28395 and RR42_RS28400 are separated by 56 nucleotides RR42_RS28400 and RR42_RS28405 are separated by 13 nucleotides
RR42_RS28390: RR42_RS28390 - acetyl-CoA acetyltransferase, at 1,730,619 to 1,731,782
_RS28390
RR42_RS28395: RR42_RS28395 - DNA-binding protein, at 1,731,779 to 1,732,231
_RS28395
RR42_RS28400: RR42_RS28400 - CoA-transferase, at 1,732,288 to 1,733,463
_RS28400
RR42_RS28405: RR42_RS28405 - protocatechuate 3,4-dioxygenase, at 1,733,477 to 1,734,472
_RS28405
Position (kb) 1732
1733
1734 Strain fitness (log2 ratio) -1
0
1
2 at 1731.444 kb on + strand, within RR42_RS28390 at 1731.457 kb on - strand, within RR42_RS28390 at 1731.603 kb on - strand, within RR42_RS28390 at 1731.603 kb on - strand, within RR42_RS28390 at 1731.603 kb on - strand, within RR42_RS28390 at 1731.749 kb on + strand at 1731.778 kb on + strand at 1732.040 kb on + strand, within RR42_RS28395 at 1732.040 kb on + strand, within RR42_RS28395 at 1732.041 kb on - strand, within RR42_RS28395 at 1732.076 kb on + strand, within RR42_RS28395 at 1732.077 kb on - strand, within RR42_RS28395 at 1732.077 kb on - strand, within RR42_RS28395 at 1732.079 kb on - strand, within RR42_RS28395 at 1732.450 kb on + strand, within RR42_RS28400 at 1732.633 kb on + strand, within RR42_RS28400 at 1733.003 kb on - strand, within RR42_RS28400 at 1733.453 kb on - strand at 1733.472 kb on + strand at 1733.472 kb on + strand at 1733.472 kb on + strand at 1733.473 kb on - strand at 1733.634 kb on + strand, within RR42_RS28405 at 1733.635 kb on - strand, within RR42_RS28405 at 1733.687 kb on + strand, within RR42_RS28405 at 1733.819 kb on + strand, within RR42_RS28405 at 1733.820 kb on - strand, within RR42_RS28405 at 1734.401 kb on + strand at 1734.402 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 1,731,444 + RR42_RS28390 0.71 -0.0 1,731,457 - RR42_RS28390 0.72 +0.2 1,731,603 - RR42_RS28390 0.85 -1.0 1,731,603 - RR42_RS28390 0.85 -0.4 1,731,603 - RR42_RS28390 0.85 -1.2 1,731,749 + +0.7 1,731,778 + +0.7 1,732,040 + RR42_RS28395 0.58 +0.5 1,732,040 + RR42_RS28395 0.58 +0.1 1,732,041 - RR42_RS28395 0.58 +0.5 1,732,076 + RR42_RS28395 0.66 -0.4 1,732,077 - RR42_RS28395 0.66 -0.4 1,732,077 - RR42_RS28395 0.66 +0.7 1,732,079 - RR42_RS28395 0.66 -0.6 1,732,450 + RR42_RS28400 0.14 +0.6 1,732,633 + RR42_RS28400 0.29 -0.7 1,733,003 - RR42_RS28400 0.61 +2.1 1,733,453 - -0.3 1,733,472 + +0.5 1,733,472 + -0.6 1,733,472 + +0.9 1,733,473 - -0.9 1,733,634 + RR42_RS28405 0.16 -0.4 1,733,635 - RR42_RS28405 0.16 -0.1 1,733,687 + RR42_RS28405 0.21 +0.1 1,733,819 + RR42_RS28405 0.34 -0.1 1,733,820 - RR42_RS28405 0.34 +0.1 1,734,401 + +0.5 1,734,402 - -1.6
Or see this region's nucleotide sequence