Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS26715 and RR42_RS26720 overlap by 97 nucleotides RR42_RS26720 and RR42_RS26725 overlap by 44 nucleotides RR42_RS26725 and RR42_RS26730 are separated by 7 nucleotides RR42_RS26730 and RR42_RS26735 are separated by 29 nucleotides RR42_RS26735 and RR42_RS26740 are separated by 28 nucleotides
RR42_RS26715: RR42_RS26715 - sulfonate ABC transporter substrate-binding protein, at 1,350,850 to 1,351,818
_RS26715
RR42_RS26720: RR42_RS26720 - hypothetical protein, at 1,351,722 to 1,351,940
_RS26720
RR42_RS26725: RR42_RS26725 - diguanylate cyclase, at 1,351,897 to 1,352,634
_RS26725
RR42_RS26730: RR42_RS26730 - Fe-S oxidoreductase, at 1,352,642 to 1,353,031
_RS26730
RR42_RS26735: RR42_RS26735 - hypothetical protein, at 1,353,061 to 1,353,630
_RS26735
RR42_RS26740: RR42_RS26740 - protein serine/threonine phosphatase, at 1,353,659 to 1,354,894
_RS26740
Position (kb)
1352
1353
1354 Strain fitness (log2 ratio)
-1
0
1 at 1351.723 kb on + strand at 1351.723 kb on + strand at 1351.723 kb on + strand at 1351.723 kb on + strand at 1351.970 kb on + strand at 1351.994 kb on + strand, within RR42_RS26725 at 1352.249 kb on - strand, within RR42_RS26725 at 1352.476 kb on + strand, within RR42_RS26725 at 1352.647 kb on + strand at 1352.742 kb on + strand, within RR42_RS26730 at 1352.835 kb on + strand, within RR42_RS26730 at 1352.836 kb on - strand, within RR42_RS26730 at 1352.886 kb on + strand, within RR42_RS26730 at 1352.917 kb on - strand, within RR42_RS26730 at 1353.125 kb on + strand, within RR42_RS26735 at 1353.413 kb on + strand, within RR42_RS26735 at 1353.413 kb on + strand, within RR42_RS26735 at 1353.554 kb on + strand, within RR42_RS26735 at 1353.555 kb on - strand, within RR42_RS26735 at 1353.602 kb on + strand at 1353.622 kb on - strand at 1353.639 kb on + strand at 1353.640 kb on - strand at 1353.642 kb on - strand at 1353.661 kb on - strand at 1353.726 kb on + strand at 1353.737 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 1,351,723 + +0.3 1,351,723 + +0.7 1,351,723 + -0.1 1,351,723 + +0.1 1,351,970 + -0.1 1,351,994 + RR42_RS26725 0.13 -0.2 1,352,249 - RR42_RS26725 0.48 +1.7 1,352,476 + RR42_RS26725 0.78 +0.2 1,352,647 + -0.1 1,352,742 + RR42_RS26730 0.26 +0.2 1,352,835 + RR42_RS26730 0.49 +0.5 1,352,836 - RR42_RS26730 0.50 +0.7 1,352,886 + RR42_RS26730 0.63 +1.5 1,352,917 - RR42_RS26730 0.71 -0.3 1,353,125 + RR42_RS26735 0.11 +0.5 1,353,413 + RR42_RS26735 0.62 -0.2 1,353,413 + RR42_RS26735 0.62 +0.2 1,353,554 + RR42_RS26735 0.86 -0.0 1,353,555 - RR42_RS26735 0.87 +0.6 1,353,602 + +0.6 1,353,622 - -0.1 1,353,639 + +0.7 1,353,640 - -0.7 1,353,642 - -0.8 1,353,661 - +0.3 1,353,726 + -0.8 1,353,737 + -1.2
Or see this region's nucleotide sequence