Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS25380

Experiment: R2A with Chloride 300 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS25370 and RR42_RS25375 are separated by 23 nucleotidesRR42_RS25375 and RR42_RS25380 are separated by 11 nucleotidesRR42_RS25380 and RR42_RS25385 are separated by 72 nucleotides RR42_RS25370: RR42_RS25370 - MFS transporter, at 1,030,835 to 1,032,205 _RS25370 RR42_RS25375: RR42_RS25375 - 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, at 1,032,229 to 1,032,987 _RS25375 RR42_RS25380: RR42_RS25380 - acetyl-CoA acetyltransferase, at 1,032,999 to 1,034,198 _RS25380 RR42_RS25385: RR42_RS25385 - cyclic nucleotide-binding protein, at 1,034,271 to 1,034,975 _RS25385 Position (kb) 1032 1033 1034 1035Strain fitness (log2 ratio) -3 -2 -1 0 1at 1032.043 kb on + strand, within RR42_RS25370at 1032.160 kb on + strandat 1032.183 kb on + strandat 1032.183 kb on + strandat 1032.184 kb on - strandat 1032.184 kb on - strandat 1032.184 kb on - strandat 1032.184 kb on - strandat 1032.204 kb on - strandat 1032.204 kb on - strandat 1032.252 kb on + strandat 1032.253 kb on - strandat 1032.294 kb on - strandat 1032.294 kb on - strandat 1032.294 kb on - strandat 1032.430 kb on + strand, within RR42_RS25375at 1032.430 kb on + strand, within RR42_RS25375at 1032.431 kb on - strand, within RR42_RS25375at 1032.431 kb on - strand, within RR42_RS25375at 1032.431 kb on - strand, within RR42_RS25375at 1032.431 kb on - strand, within RR42_RS25375at 1032.455 kb on + strand, within RR42_RS25375at 1032.700 kb on + strand, within RR42_RS25375at 1032.876 kb on + strand, within RR42_RS25375at 1032.931 kb on + strandat 1032.931 kb on + strandat 1033.377 kb on + strand, within RR42_RS25380at 1033.476 kb on - strand, within RR42_RS25380at 1033.524 kb on + strand, within RR42_RS25380at 1033.706 kb on + strand, within RR42_RS25380at 1034.138 kb on + strandat 1034.138 kb on + strandat 1034.139 kb on - strandat 1034.153 kb on + strandat 1034.322 kb on + strandat 1034.322 kb on + strandat 1034.323 kb on - strandat 1034.332 kb on + strandat 1034.545 kb on + strand, within RR42_RS25385at 1034.545 kb on + strand, within RR42_RS25385at 1034.545 kb on + strand, within RR42_RS25385at 1034.545 kb on + strand, within RR42_RS25385at 1034.546 kb on - strand, within RR42_RS25385at 1034.546 kb on - strand, within RR42_RS25385at 1034.546 kb on - strand, within RR42_RS25385at 1034.546 kb on - strand, within RR42_RS25385at 1034.548 kb on + strand, within RR42_RS25385at 1034.581 kb on + strand, within RR42_RS25385at 1034.581 kb on + strand, within RR42_RS25385at 1034.582 kb on - strand, within RR42_RS25385at 1034.674 kb on + strand, within RR42_RS25385at 1034.675 kb on - strand, within RR42_RS25385at 1034.748 kb on - strand, within RR42_RS25385at 1034.748 kb on - strand, within RR42_RS25385at 1034.748 kb on - strand, within RR42_RS25385at 1034.866 kb on + strand, within RR42_RS25385at 1034.867 kb on - strand, within RR42_RS25385at 1035.176 kb on + strandat 1035.176 kb on + strandat 1035.176 kb on + strandat 1035.176 kb on + strandat 1035.176 kb on + strandat 1035.176 kb on + strandat 1035.176 kb on + strandat 1035.177 kb on - strandat 1035.177 kb on - strandat 1035.177 kb on - strandat 1035.177 kb on - strandat 1035.177 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM
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1,032,043 + RR42_RS25370 0.88 -1.0
1,032,160 + -1.2
1,032,183 + +0.1
1,032,183 + -1.2
1,032,184 - +0.2
1,032,184 - -1.1
1,032,184 - -0.2
1,032,184 - -3.0
1,032,204 - -0.8
1,032,204 - -0.0
1,032,252 + +0.4
1,032,253 - +0.5
1,032,294 - -0.2
1,032,294 - +0.7
1,032,294 - -0.0
1,032,430 + RR42_RS25375 0.26 -0.4
1,032,430 + RR42_RS25375 0.26 +0.7
1,032,431 - RR42_RS25375 0.27 +0.8
1,032,431 - RR42_RS25375 0.27 -2.0
1,032,431 - RR42_RS25375 0.27 -0.1
1,032,431 - RR42_RS25375 0.27 -0.2
1,032,455 + RR42_RS25375 0.30 -0.6
1,032,700 + RR42_RS25375 0.62 -0.2
1,032,876 + RR42_RS25375 0.85 -0.3
1,032,931 + +1.1
1,032,931 + -0.0
1,033,377 + RR42_RS25380 0.32 -0.1
1,033,476 - RR42_RS25380 0.40 +1.5
1,033,524 + RR42_RS25380 0.44 -0.1
1,033,706 + RR42_RS25380 0.59 +0.7
1,034,138 + +1.1
1,034,138 + +1.3
1,034,139 - -0.0
1,034,153 + +0.1
1,034,322 + -2.9
1,034,322 + -1.2
1,034,323 - -0.7
1,034,332 + -0.1
1,034,545 + RR42_RS25385 0.39 +0.5
1,034,545 + RR42_RS25385 0.39 -1.5
1,034,545 + RR42_RS25385 0.39 -0.2
1,034,545 + RR42_RS25385 0.39 +0.4
1,034,546 - RR42_RS25385 0.39 -0.3
1,034,546 - RR42_RS25385 0.39 +0.6
1,034,546 - RR42_RS25385 0.39 +0.2
1,034,546 - RR42_RS25385 0.39 +0.4
1,034,548 + RR42_RS25385 0.39 -0.2
1,034,581 + RR42_RS25385 0.44 -0.3
1,034,581 + RR42_RS25385 0.44 +0.1
1,034,582 - RR42_RS25385 0.44 -0.6
1,034,674 + RR42_RS25385 0.57 +0.2
1,034,675 - RR42_RS25385 0.57 -1.5
1,034,748 - RR42_RS25385 0.68 -0.5
1,034,748 - RR42_RS25385 0.68 -0.4
1,034,748 - RR42_RS25385 0.68 -0.0
1,034,866 + RR42_RS25385 0.84 -2.2
1,034,867 - RR42_RS25385 0.85 +0.2
1,035,176 + +0.0
1,035,176 + -0.4
1,035,176 + -2.0
1,035,176 + +0.7
1,035,176 + +0.3
1,035,176 + +0.1
1,035,176 + +0.1
1,035,177 - -0.2
1,035,177 - -0.6
1,035,177 - -0.5
1,035,177 - +0.9
1,035,177 - -0.2

Or see this region's nucleotide sequence