Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25370 and RR42_RS25375 are separated by 23 nucleotides RR42_RS25375 and RR42_RS25380 are separated by 11 nucleotides RR42_RS25380 and RR42_RS25385 are separated by 72 nucleotides
RR42_RS25370: RR42_RS25370 - MFS transporter, at 1,030,835 to 1,032,205
_RS25370
RR42_RS25375: RR42_RS25375 - 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, at 1,032,229 to 1,032,987
_RS25375
RR42_RS25380: RR42_RS25380 - acetyl-CoA acetyltransferase, at 1,032,999 to 1,034,198
_RS25380
RR42_RS25385: RR42_RS25385 - cyclic nucleotide-binding protein, at 1,034,271 to 1,034,975
_RS25385
Position (kb)
1032
1033
1034
1035 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1032.043 kb on + strand, within RR42_RS25370 at 1032.160 kb on + strand at 1032.183 kb on + strand at 1032.183 kb on + strand at 1032.184 kb on - strand at 1032.184 kb on - strand at 1032.184 kb on - strand at 1032.184 kb on - strand at 1032.204 kb on - strand at 1032.204 kb on - strand at 1032.252 kb on + strand at 1032.253 kb on - strand at 1032.294 kb on - strand at 1032.294 kb on - strand at 1032.294 kb on - strand at 1032.430 kb on + strand, within RR42_RS25375 at 1032.430 kb on + strand, within RR42_RS25375 at 1032.431 kb on - strand, within RR42_RS25375 at 1032.431 kb on - strand, within RR42_RS25375 at 1032.431 kb on - strand, within RR42_RS25375 at 1032.431 kb on - strand, within RR42_RS25375 at 1032.455 kb on + strand, within RR42_RS25375 at 1032.700 kb on + strand, within RR42_RS25375 at 1032.876 kb on + strand, within RR42_RS25375 at 1032.931 kb on + strand at 1032.931 kb on + strand at 1033.377 kb on + strand, within RR42_RS25380 at 1033.476 kb on - strand, within RR42_RS25380 at 1033.524 kb on + strand, within RR42_RS25380 at 1033.706 kb on + strand, within RR42_RS25380 at 1034.138 kb on + strand at 1034.138 kb on + strand at 1034.139 kb on - strand at 1034.153 kb on + strand at 1034.322 kb on + strand at 1034.322 kb on + strand at 1034.323 kb on - strand at 1034.332 kb on + strand at 1034.545 kb on + strand, within RR42_RS25385 at 1034.545 kb on + strand, within RR42_RS25385 at 1034.545 kb on + strand, within RR42_RS25385 at 1034.545 kb on + strand, within RR42_RS25385 at 1034.546 kb on - strand, within RR42_RS25385 at 1034.546 kb on - strand, within RR42_RS25385 at 1034.546 kb on - strand, within RR42_RS25385 at 1034.546 kb on - strand, within RR42_RS25385 at 1034.548 kb on + strand, within RR42_RS25385 at 1034.581 kb on + strand, within RR42_RS25385 at 1034.581 kb on + strand, within RR42_RS25385 at 1034.582 kb on - strand, within RR42_RS25385 at 1034.674 kb on + strand, within RR42_RS25385 at 1034.675 kb on - strand, within RR42_RS25385 at 1034.748 kb on - strand, within RR42_RS25385 at 1034.748 kb on - strand, within RR42_RS25385 at 1034.748 kb on - strand, within RR42_RS25385 at 1034.866 kb on + strand, within RR42_RS25385 at 1034.867 kb on - strand, within RR42_RS25385 at 1035.176 kb on + strand at 1035.176 kb on + strand at 1035.176 kb on + strand at 1035.176 kb on + strand at 1035.176 kb on + strand at 1035.176 kb on + strand at 1035.176 kb on + strand at 1035.177 kb on - strand at 1035.177 kb on - strand at 1035.177 kb on - strand at 1035.177 kb on - strand at 1035.177 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 1,032,043 + RR42_RS25370 0.88 -1.0 1,032,160 + -1.2 1,032,183 + +0.1 1,032,183 + -1.2 1,032,184 - +0.2 1,032,184 - -1.1 1,032,184 - -0.2 1,032,184 - -3.0 1,032,204 - -0.8 1,032,204 - -0.0 1,032,252 + +0.4 1,032,253 - +0.5 1,032,294 - -0.2 1,032,294 - +0.7 1,032,294 - -0.0 1,032,430 + RR42_RS25375 0.26 -0.4 1,032,430 + RR42_RS25375 0.26 +0.7 1,032,431 - RR42_RS25375 0.27 +0.8 1,032,431 - RR42_RS25375 0.27 -2.0 1,032,431 - RR42_RS25375 0.27 -0.1 1,032,431 - RR42_RS25375 0.27 -0.2 1,032,455 + RR42_RS25375 0.30 -0.6 1,032,700 + RR42_RS25375 0.62 -0.2 1,032,876 + RR42_RS25375 0.85 -0.3 1,032,931 + +1.1 1,032,931 + -0.0 1,033,377 + RR42_RS25380 0.32 -0.1 1,033,476 - RR42_RS25380 0.40 +1.5 1,033,524 + RR42_RS25380 0.44 -0.1 1,033,706 + RR42_RS25380 0.59 +0.7 1,034,138 + +1.1 1,034,138 + +1.3 1,034,139 - -0.0 1,034,153 + +0.1 1,034,322 + -2.9 1,034,322 + -1.2 1,034,323 - -0.7 1,034,332 + -0.1 1,034,545 + RR42_RS25385 0.39 +0.5 1,034,545 + RR42_RS25385 0.39 -1.5 1,034,545 + RR42_RS25385 0.39 -0.2 1,034,545 + RR42_RS25385 0.39 +0.4 1,034,546 - RR42_RS25385 0.39 -0.3 1,034,546 - RR42_RS25385 0.39 +0.6 1,034,546 - RR42_RS25385 0.39 +0.2 1,034,546 - RR42_RS25385 0.39 +0.4 1,034,548 + RR42_RS25385 0.39 -0.2 1,034,581 + RR42_RS25385 0.44 -0.3 1,034,581 + RR42_RS25385 0.44 +0.1 1,034,582 - RR42_RS25385 0.44 -0.6 1,034,674 + RR42_RS25385 0.57 +0.2 1,034,675 - RR42_RS25385 0.57 -1.5 1,034,748 - RR42_RS25385 0.68 -0.5 1,034,748 - RR42_RS25385 0.68 -0.4 1,034,748 - RR42_RS25385 0.68 -0.0 1,034,866 + RR42_RS25385 0.84 -2.2 1,034,867 - RR42_RS25385 0.85 +0.2 1,035,176 + +0.0 1,035,176 + -0.4 1,035,176 + -2.0 1,035,176 + +0.7 1,035,176 + +0.3 1,035,176 + +0.1 1,035,176 + +0.1 1,035,177 - -0.2 1,035,177 - -0.6 1,035,177 - -0.5 1,035,177 - +0.9 1,035,177 - -0.2
Or see this region's nucleotide sequence