Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS24510 and RR42_RS24515 overlap by 4 nucleotides RR42_RS24515 and RR42_RS24520 overlap by 4 nucleotides RR42_RS24520 and RR42_RS24525 are separated by 938 nucleotides
RR42_RS24510: RR42_RS24510 - hypothetical protein, at 831,928 to 832,356
_RS24510
RR42_RS24515: RR42_RS24515 - hypothetical protein, at 832,353 to 833,003
_RS24515
RR42_RS24520: RR42_RS24520 - hypothetical protein, at 833,000 to 833,551
_RS24520
RR42_RS24525: RR42_RS24525 - hypothetical protein, at 834,490 to 835,209
_RS24525
Position (kb) 832
833
834 Strain fitness (log2 ratio) -2
-1
0
1
2 at 832.212 kb on + strand, within RR42_RS24510 at 832.213 kb on - strand, within RR42_RS24510 at 832.670 kb on + strand, within RR42_RS24515 at 832.671 kb on - strand, within RR42_RS24515 at 832.756 kb on + strand, within RR42_RS24515 at 832.756 kb on + strand, within RR42_RS24515 at 832.793 kb on - strand, within RR42_RS24515 at 833.035 kb on - strand at 833.035 kb on - strand at 833.035 kb on - strand at 833.052 kb on + strand at 833.053 kb on - strand at 833.053 kb on - strand at 833.053 kb on - strand at 833.053 kb on - strand at 833.194 kb on - strand, within RR42_RS24520 at 833.194 kb on - strand, within RR42_RS24520 at 833.280 kb on + strand, within RR42_RS24520 at 833.281 kb on - strand, within RR42_RS24520 at 833.569 kb on - strand at 833.635 kb on + strand at 833.635 kb on + strand at 833.651 kb on + strand at 833.958 kb on + strand at 833.958 kb on + strand at 833.958 kb on + strand at 833.959 kb on - strand at 833.981 kb on + strand at 834.129 kb on - strand at 834.129 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 832,212 + RR42_RS24510 0.66 +1.3 832,213 - RR42_RS24510 0.66 +0.9 832,670 + RR42_RS24515 0.49 +0.5 832,671 - RR42_RS24515 0.49 -2.2 832,756 + RR42_RS24515 0.62 -0.7 832,756 + RR42_RS24515 0.62 +0.2 832,793 - RR42_RS24515 0.68 +0.5 833,035 - +0.6 833,035 - +0.7 833,035 - +2.0 833,052 + -0.0 833,053 - +0.0 833,053 - +0.8 833,053 - +0.1 833,053 - +0.3 833,194 - RR42_RS24520 0.35 +1.6 833,194 - RR42_RS24520 0.35 +0.7 833,280 + RR42_RS24520 0.51 +0.1 833,281 - RR42_RS24520 0.51 +0.5 833,569 - -1.5 833,635 + +0.1 833,635 + -1.8 833,651 + -0.2 833,958 + -0.8 833,958 + -0.3 833,958 + -0.6 833,959 - -0.3 833,981 + -0.2 834,129 - +1.3 834,129 - -0.4
Or see this region's nucleotide sequence