Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23865 and RR42_RS23870 are separated by 83 nucleotides RR42_RS23870 and RR42_RS23875 are separated by 51 nucleotides RR42_RS23875 and RR42_RS23880 are separated by 66 nucleotides RR42_RS23880 and RR42_RS23885 are separated by 343 nucleotides
RR42_RS23865: RR42_RS23865 - hypothetical protein, at 684,111 to 684,407
_RS23865
RR42_RS23870: RR42_RS23870 - hypothetical protein, at 684,491 to 684,784
_RS23870
RR42_RS23875: RR42_RS23875 - hypothetical protein, at 684,836 to 685,132
_RS23875
RR42_RS23880: RR42_RS23880 - hypothetical protein, at 685,199 to 685,399
_RS23880
RR42_RS23885: RR42_RS23885 - hypothetical protein, at 685,743 to 686,480
_RS23885
Position (kb)
685
686 Strain fitness (log2 ratio)
-2
-1
0
1 at 684.584 kb on + strand, within RR42_RS23870 at 684.584 kb on + strand, within RR42_RS23870 at 684.585 kb on - strand, within RR42_RS23870 at 684.603 kb on - strand, within RR42_RS23870 at 684.782 kb on + strand at 684.782 kb on + strand at 684.783 kb on - strand at 684.916 kb on - strand, within RR42_RS23875 at 684.941 kb on + strand, within RR42_RS23875 at 684.942 kb on - strand, within RR42_RS23875 at 685.043 kb on + strand, within RR42_RS23875 at 685.061 kb on + strand, within RR42_RS23875 at 685.062 kb on - strand, within RR42_RS23875 at 685.068 kb on - strand, within RR42_RS23875 at 685.068 kb on - strand, within RR42_RS23875 at 685.130 kb on + strand at 685.225 kb on + strand, within RR42_RS23880 at 685.225 kb on + strand, within RR42_RS23880 at 685.226 kb on - strand, within RR42_RS23880 at 685.448 kb on + strand at 685.449 kb on - strand at 685.586 kb on + strand at 685.586 kb on + strand at 685.586 kb on + strand at 685.587 kb on - strand at 685.587 kb on - strand at 685.588 kb on + strand at 685.662 kb on - strand at 685.736 kb on - strand at 685.736 kb on - strand at 685.889 kb on - strand, within RR42_RS23885 at 685.937 kb on - strand, within RR42_RS23885 at 685.937 kb on - strand, within RR42_RS23885 at 686.169 kb on + strand, within RR42_RS23885 at 686.169 kb on + strand, within RR42_RS23885 at 686.169 kb on + strand, within RR42_RS23885 at 686.169 kb on + strand, within RR42_RS23885 at 686.170 kb on - strand, within RR42_RS23885 at 686.170 kb on - strand, within RR42_RS23885 at 686.170 kb on - strand, within RR42_RS23885 at 686.170 kb on - strand, within RR42_RS23885 at 686.170 kb on - strand, within RR42_RS23885 at 686.170 kb on - strand, within RR42_RS23885 at 686.294 kb on + strand, within RR42_RS23885 at 686.294 kb on + strand, within RR42_RS23885 at 686.295 kb on - strand, within RR42_RS23885 at 686.295 kb on - strand, within RR42_RS23885 at 686.298 kb on + strand, within RR42_RS23885 at 686.298 kb on + strand, within RR42_RS23885 at 686.298 kb on + strand, within RR42_RS23885 at 686.298 kb on + strand, within RR42_RS23885 at 686.382 kb on + strand, within RR42_RS23885
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 684,584 + RR42_RS23870 0.32 -0.2 684,584 + RR42_RS23870 0.32 +0.1 684,585 - RR42_RS23870 0.32 +0.6 684,603 - RR42_RS23870 0.38 +0.7 684,782 + -0.4 684,782 + -0.5 684,783 - +0.3 684,916 - RR42_RS23875 0.27 -1.0 684,941 + RR42_RS23875 0.35 -0.0 684,942 - RR42_RS23875 0.36 -0.9 685,043 + RR42_RS23875 0.70 +0.1 685,061 + RR42_RS23875 0.76 +0.5 685,062 - RR42_RS23875 0.76 -0.2 685,068 - RR42_RS23875 0.78 -2.0 685,068 - RR42_RS23875 0.78 +0.4 685,130 + +0.4 685,225 + RR42_RS23880 0.13 -0.0 685,225 + RR42_RS23880 0.13 +1.6 685,226 - RR42_RS23880 0.13 +1.1 685,448 + -1.0 685,449 - -0.2 685,586 + -0.5 685,586 + +0.3 685,586 + -0.0 685,587 - -0.5 685,587 - -0.9 685,588 + -0.3 685,662 - +0.7 685,736 - -0.0 685,736 - -1.0 685,889 - RR42_RS23885 0.20 -0.5 685,937 - RR42_RS23885 0.26 -0.7 685,937 - RR42_RS23885 0.26 -0.2 686,169 + RR42_RS23885 0.58 -0.3 686,169 + RR42_RS23885 0.58 -0.9 686,169 + RR42_RS23885 0.58 +0.6 686,169 + RR42_RS23885 0.58 -0.9 686,170 - RR42_RS23885 0.58 -1.2 686,170 - RR42_RS23885 0.58 +1.6 686,170 - RR42_RS23885 0.58 -0.1 686,170 - RR42_RS23885 0.58 +0.0 686,170 - RR42_RS23885 0.58 -0.1 686,170 - RR42_RS23885 0.58 -1.2 686,294 + RR42_RS23885 0.75 -0.7 686,294 + RR42_RS23885 0.75 -0.5 686,295 - RR42_RS23885 0.75 -0.6 686,295 - RR42_RS23885 0.75 -2.0 686,298 + RR42_RS23885 0.75 +0.1 686,298 + RR42_RS23885 0.75 +1.0 686,298 + RR42_RS23885 0.75 -0.1 686,298 + RR42_RS23885 0.75 +0.9 686,382 + RR42_RS23885 0.87 +0.3
Or see this region's nucleotide sequence