Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23410 and RR42_RS23415 are separated by 225 nucleotides RR42_RS23415 and RR42_RS23420 are separated by 54 nucleotides RR42_RS23420 and RR42_RS23425 are separated by 44 nucleotides
RR42_RS23410: RR42_RS23410 - hypothetical protein, at 579,749 to 580,648
_RS23410
RR42_RS23415: RR42_RS23415 - 4-carboxymuconolactone decarboxylase, at 580,874 to 581,428
_RS23415
RR42_RS23420: RR42_RS23420 - 3-oxoacyl-ACP reductase, at 581,483 to 582,283
_RS23420
RR42_RS23425: RR42_RS23425 - hypothetical protein, at 582,328 to 583,323
_RS23425
Position (kb) 581
582
583 Strain fitness (log2 ratio) -2
-1
0
1
2 at 580.486 kb on + strand, within RR42_RS23410 at 580.697 kb on - strand at 580.727 kb on + strand at 580.728 kb on - strand at 580.728 kb on - strand at 580.762 kb on + strand at 580.965 kb on + strand, within RR42_RS23415 at 580.965 kb on + strand, within RR42_RS23415 at 580.965 kb on + strand, within RR42_RS23415 at 581.033 kb on + strand, within RR42_RS23415 at 581.034 kb on - strand, within RR42_RS23415 at 581.034 kb on - strand, within RR42_RS23415 at 581.243 kb on - strand, within RR42_RS23415 at 581.282 kb on + strand, within RR42_RS23415 at 581.282 kb on + strand, within RR42_RS23415 at 581.283 kb on - strand, within RR42_RS23415 at 581.322 kb on + strand, within RR42_RS23415 at 581.348 kb on + strand, within RR42_RS23415 at 581.476 kb on - strand at 581.476 kb on - strand at 581.834 kb on - strand, within RR42_RS23420 at 581.966 kb on + strand, within RR42_RS23420 at 582.020 kb on + strand, within RR42_RS23420 at 582.399 kb on - strand at 582.537 kb on + strand, within RR42_RS23425 at 582.622 kb on + strand, within RR42_RS23425 at 582.623 kb on - strand, within RR42_RS23425 at 582.624 kb on - strand, within RR42_RS23425 at 582.625 kb on - strand, within RR42_RS23425 at 582.711 kb on - strand, within RR42_RS23425 at 583.059 kb on + strand, within RR42_RS23425 at 583.060 kb on - strand, within RR42_RS23425 at 583.273 kb on + strand at 583.274 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 580,486 + RR42_RS23410 0.82 -1.1 580,697 - -1.3 580,727 + +0.0 580,728 - +2.0 580,728 - -0.9 580,762 + -1.0 580,965 + RR42_RS23415 0.16 -2.0 580,965 + RR42_RS23415 0.16 -0.9 580,965 + RR42_RS23415 0.16 -1.5 581,033 + RR42_RS23415 0.29 +1.3 581,034 - RR42_RS23415 0.29 -0.3 581,034 - RR42_RS23415 0.29 +0.1 581,243 - RR42_RS23415 0.66 -0.5 581,282 + RR42_RS23415 0.74 +0.1 581,282 + RR42_RS23415 0.74 -0.7 581,283 - RR42_RS23415 0.74 -0.3 581,322 + RR42_RS23415 0.81 -0.0 581,348 + RR42_RS23415 0.85 -0.4 581,476 - -0.8 581,476 - -0.3 581,834 - RR42_RS23420 0.44 +0.1 581,966 + RR42_RS23420 0.60 +1.3 582,020 + RR42_RS23420 0.67 +0.3 582,399 - -0.7 582,537 + RR42_RS23425 0.21 +0.5 582,622 + RR42_RS23425 0.30 -0.6 582,623 - RR42_RS23425 0.30 +0.2 582,624 - RR42_RS23425 0.30 +1.5 582,625 - RR42_RS23425 0.30 -0.2 582,711 - RR42_RS23425 0.38 +0.5 583,059 + RR42_RS23425 0.73 -0.5 583,060 - RR42_RS23425 0.73 +0.7 583,273 + +0.1 583,274 - +0.6
Or see this region's nucleotide sequence