Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23325 and RR42_RS23330 are separated by 53 nucleotides RR42_RS23330 and RR42_RS23335 overlap by 9 nucleotides RR42_RS23335 and RR42_RS23340 are separated by 491 nucleotides
RR42_RS23325: RR42_RS23325 - class D beta-lactamase, at 564,454 to 565,239
_RS23325
RR42_RS23330: RR42_RS23330 - class A beta-lactamase, at 565,293 to 566,180
_RS23330
RR42_RS23335: RR42_RS23335 - hypothetical protein, at 566,172 to 566,552
_RS23335
RR42_RS23340: RR42_RS23340 - RNA ligase, at 567,044 to 568,270
_RS23340
Position (kb)
566
567 Strain fitness (log2 ratio)
-2
-1
0
1 at 565.596 kb on - strand, within RR42_RS23330 at 565.596 kb on - strand, within RR42_RS23330 at 565.695 kb on + strand, within RR42_RS23330 at 565.952 kb on + strand, within RR42_RS23330 at 565.952 kb on + strand, within RR42_RS23330 at 566.029 kb on - strand, within RR42_RS23330 at 566.174 kb on - strand at 566.178 kb on + strand at 566.179 kb on - strand at 566.179 kb on - strand at 566.179 kb on - strand at 566.179 kb on - strand at 566.179 kb on - strand at 566.224 kb on - strand, within RR42_RS23335 at 566.274 kb on - strand, within RR42_RS23335 at 566.347 kb on + strand, within RR42_RS23335 at 566.347 kb on + strand, within RR42_RS23335 at 566.347 kb on + strand, within RR42_RS23335 at 566.347 kb on + strand, within RR42_RS23335 at 566.348 kb on - strand, within RR42_RS23335 at 566.348 kb on - strand, within RR42_RS23335 at 566.849 kb on + strand at 566.850 kb on - strand at 566.850 kb on - strand at 566.919 kb on - strand at 566.921 kb on + strand at 566.921 kb on + strand at 566.921 kb on + strand at 566.921 kb on + strand at 566.921 kb on + strand at 566.922 kb on - strand at 566.922 kb on - strand at 566.922 kb on - strand at 566.922 kb on - strand at 566.945 kb on + strand at 566.945 kb on + strand at 566.945 kb on + strand at 566.946 kb on - strand at 566.981 kb on + strand at 566.982 kb on - strand at 566.982 kb on - strand at 566.982 kb on - strand at 566.982 kb on - strand at 567.011 kb on + strand at 567.060 kb on - strand at 567.067 kb on - strand at 567.067 kb on - strand at 567.067 kb on - strand at 567.067 kb on - strand at 567.202 kb on - strand, within RR42_RS23340 at 567.202 kb on - strand, within RR42_RS23340 at 567.497 kb on + strand, within RR42_RS23340 at 567.498 kb on - strand, within RR42_RS23340
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 565,596 - RR42_RS23330 0.34 +1.6 565,596 - RR42_RS23330 0.34 +0.2 565,695 + RR42_RS23330 0.45 -0.7 565,952 + RR42_RS23330 0.74 -0.2 565,952 + RR42_RS23330 0.74 +0.7 566,029 - RR42_RS23330 0.83 +0.0 566,174 - -0.5 566,178 + +0.2 566,179 - +0.4 566,179 - +0.5 566,179 - +0.2 566,179 - -0.8 566,179 - -1.5 566,224 - RR42_RS23335 0.14 -1.1 566,274 - RR42_RS23335 0.27 -2.5 566,347 + RR42_RS23335 0.46 +0.6 566,347 + RR42_RS23335 0.46 -0.2 566,347 + RR42_RS23335 0.46 -1.4 566,347 + RR42_RS23335 0.46 -0.6 566,348 - RR42_RS23335 0.46 -0.8 566,348 - RR42_RS23335 0.46 +0.5 566,849 + -0.5 566,850 - +0.1 566,850 - +1.2 566,919 - +0.1 566,921 + +0.3 566,921 + +0.1 566,921 + -0.3 566,921 + -1.1 566,921 + -0.0 566,922 - +1.0 566,922 - -0.1 566,922 - +0.5 566,922 - -1.6 566,945 + +0.6 566,945 + -1.4 566,945 + -0.9 566,946 - -0.0 566,981 + -1.6 566,982 - +0.4 566,982 - +0.5 566,982 - +0.2 566,982 - +1.4 567,011 + +0.2 567,060 - +1.5 567,067 - +0.7 567,067 - +0.7 567,067 - +0.2 567,067 - -0.1 567,202 - RR42_RS23340 0.13 +0.2 567,202 - RR42_RS23340 0.13 -1.1 567,497 + RR42_RS23340 0.37 +0.2 567,498 - RR42_RS23340 0.37 +0.9
Or see this region's nucleotide sequence