Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22710 and RR42_RS22715 are separated by 225 nucleotides RR42_RS22715 and RR42_RS22720 overlap by 17 nucleotides
RR42_RS22710: RR42_RS22710 - short-chain dehydrogenase, at 428,992 to 429,741
_RS22710
RR42_RS22715: RR42_RS22715 - AMP-binding protein, at 429,967 to 431,616
_RS22715
RR42_RS22720: RR42_RS22720 - hypothetical protein, at 431,600 to 431,977
_RS22720
Position (kb)
429
430
431
432 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 428.967 kb on - strand at 428.967 kb on - strand at 429.180 kb on - strand, within RR42_RS22710 at 429.293 kb on + strand, within RR42_RS22710 at 429.782 kb on + strand at 429.782 kb on + strand at 429.782 kb on + strand at 429.801 kb on + strand at 429.968 kb on + strand at 429.969 kb on - strand at 430.080 kb on - strand at 430.148 kb on + strand, within RR42_RS22715 at 430.317 kb on + strand, within RR42_RS22715 at 430.367 kb on + strand, within RR42_RS22715 at 430.367 kb on + strand, within RR42_RS22715 at 430.368 kb on - strand, within RR42_RS22715 at 430.368 kb on - strand, within RR42_RS22715 at 430.370 kb on + strand, within RR42_RS22715 at 430.371 kb on - strand, within RR42_RS22715 at 430.441 kb on - strand, within RR42_RS22715 at 430.604 kb on + strand, within RR42_RS22715 at 430.832 kb on + strand, within RR42_RS22715 at 430.877 kb on + strand, within RR42_RS22715 at 430.877 kb on + strand, within RR42_RS22715 at 430.878 kb on - strand, within RR42_RS22715 at 430.880 kb on + strand, within RR42_RS22715 at 430.880 kb on + strand, within RR42_RS22715 at 430.880 kb on + strand, within RR42_RS22715 at 430.880 kb on + strand, within RR42_RS22715 at 430.881 kb on - strand, within RR42_RS22715 at 430.881 kb on - strand, within RR42_RS22715 at 430.889 kb on + strand, within RR42_RS22715 at 430.890 kb on - strand, within RR42_RS22715 at 430.956 kb on + strand, within RR42_RS22715 at 430.956 kb on + strand, within RR42_RS22715 at 430.956 kb on + strand, within RR42_RS22715 at 430.957 kb on - strand, within RR42_RS22715 at 431.161 kb on + strand, within RR42_RS22715 at 431.161 kb on + strand, within RR42_RS22715 at 431.162 kb on - strand, within RR42_RS22715 at 431.162 kb on - strand, within RR42_RS22715 at 431.162 kb on - strand, within RR42_RS22715 at 431.162 kb on - strand, within RR42_RS22715 at 431.162 kb on - strand, within RR42_RS22715 at 431.162 kb on - strand, within RR42_RS22715 at 431.179 kb on + strand, within RR42_RS22715 at 431.179 kb on + strand, within RR42_RS22715 at 431.179 kb on + strand, within RR42_RS22715 at 431.179 kb on + strand, within RR42_RS22715 at 431.180 kb on - strand, within RR42_RS22715 at 431.180 kb on - strand, within RR42_RS22715 at 431.442 kb on + strand, within RR42_RS22715 at 431.442 kb on + strand, within RR42_RS22715 at 431.442 kb on + strand, within RR42_RS22715 at 431.443 kb on - strand, within RR42_RS22715 at 431.443 kb on - strand, within RR42_RS22715 at 431.443 kb on - strand, within RR42_RS22715 at 431.443 kb on - strand, within RR42_RS22715 at 431.444 kb on + strand, within RR42_RS22715 at 431.444 kb on + strand, within RR42_RS22715 at 431.445 kb on - strand, within RR42_RS22715 at 431.637 kb on + strand at 431.638 kb on - strand, within RR42_RS22720 at 432.013 kb on + strand at 432.031 kb on + strand at 432.034 kb on + strand at 432.034 kb on + strand at 432.034 kb on + strand at 432.034 kb on + strand at 432.034 kb on + strand at 432.034 kb on + strand at 432.034 kb on + strand at 432.034 kb on + strand at 432.035 kb on - strand at 432.035 kb on - strand at 432.035 kb on - strand at 432.035 kb on - strand at 432.035 kb on - strand at 432.046 kb on + strand at 432.095 kb on - strand at 432.095 kb on - strand at 432.125 kb on - strand at 432.125 kb on - strand at 432.148 kb on + strand at 432.148 kb on + strand at 432.149 kb on - strand at 432.193 kb on + strand at 432.194 kb on - strand at 432.300 kb on - strand at 432.494 kb on + strand at 432.495 kb on - strand at 432.495 kb on - strand at 432.536 kb on + strand at 432.536 kb on + strand at 432.613 kb on + strand at 432.614 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 428,967 - +0.6 428,967 - +0.4 429,180 - RR42_RS22710 0.25 +0.5 429,293 + RR42_RS22710 0.40 +0.1 429,782 + -0.8 429,782 + +0.7 429,782 + +0.5 429,801 + -0.3 429,968 + -1.4 429,969 - -0.5 430,080 - -0.1 430,148 + RR42_RS22715 0.11 -0.0 430,317 + RR42_RS22715 0.21 -0.6 430,367 + RR42_RS22715 0.24 +2.2 430,367 + RR42_RS22715 0.24 +0.8 430,368 - RR42_RS22715 0.24 +0.5 430,368 - RR42_RS22715 0.24 -1.0 430,370 + RR42_RS22715 0.24 +0.2 430,371 - RR42_RS22715 0.24 -0.5 430,441 - RR42_RS22715 0.29 -0.9 430,604 + RR42_RS22715 0.39 +0.1 430,832 + RR42_RS22715 0.52 +0.6 430,877 + RR42_RS22715 0.55 +0.3 430,877 + RR42_RS22715 0.55 +1.0 430,878 - RR42_RS22715 0.55 +0.1 430,880 + RR42_RS22715 0.55 +0.3 430,880 + RR42_RS22715 0.55 +0.1 430,880 + RR42_RS22715 0.55 +0.9 430,880 + RR42_RS22715 0.55 +0.4 430,881 - RR42_RS22715 0.55 +0.5 430,881 - RR42_RS22715 0.55 -0.5 430,889 + RR42_RS22715 0.56 -0.5 430,890 - RR42_RS22715 0.56 -0.4 430,956 + RR42_RS22715 0.60 +0.2 430,956 + RR42_RS22715 0.60 +0.5 430,956 + RR42_RS22715 0.60 +0.6 430,957 - RR42_RS22715 0.60 +0.2 431,161 + RR42_RS22715 0.72 +0.7 431,161 + RR42_RS22715 0.72 -2.4 431,162 - RR42_RS22715 0.72 +0.4 431,162 - RR42_RS22715 0.72 +0.4 431,162 - RR42_RS22715 0.72 +0.2 431,162 - RR42_RS22715 0.72 +0.7 431,162 - RR42_RS22715 0.72 -0.3 431,162 - RR42_RS22715 0.72 +0.1 431,179 + RR42_RS22715 0.73 -1.1 431,179 + RR42_RS22715 0.73 +0.4 431,179 + RR42_RS22715 0.73 -0.2 431,179 + RR42_RS22715 0.73 -0.0 431,180 - RR42_RS22715 0.74 +1.5 431,180 - RR42_RS22715 0.74 +0.7 431,442 + RR42_RS22715 0.89 +0.3 431,442 + RR42_RS22715 0.89 +0.7 431,442 + RR42_RS22715 0.89 +0.6 431,443 - RR42_RS22715 0.89 +0.4 431,443 - RR42_RS22715 0.89 +1.1 431,443 - RR42_RS22715 0.89 -0.4 431,443 - RR42_RS22715 0.89 +0.0 431,444 + RR42_RS22715 0.90 +0.1 431,444 + RR42_RS22715 0.90 -2.0 431,445 - RR42_RS22715 0.90 +1.6 431,637 + +0.9 431,638 - RR42_RS22720 0.10 -0.5 432,013 + +0.3 432,031 + -1.6 432,034 + +0.6 432,034 + -0.2 432,034 + -0.1 432,034 + +3.0 432,034 + +0.4 432,034 + +2.6 432,034 + -0.4 432,034 + +0.5 432,035 - +1.1 432,035 - -0.3 432,035 - -0.0 432,035 - +0.8 432,035 - +0.3 432,046 + -1.0 432,095 - +0.4 432,095 - +1.4 432,125 - +2.0 432,125 - -0.6 432,148 + +0.3 432,148 + -0.0 432,149 - +0.8 432,193 + -0.5 432,194 - +0.1 432,300 - +1.6 432,494 + -0.0 432,495 - +0.6 432,495 - -0.4 432,536 + -0.0 432,536 + +0.5 432,613 + -0.1 432,614 - +0.6
Or see this region's nucleotide sequence