Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22255 and RR42_RS22260 are separated by 24 nucleotides RR42_RS22260 and RR42_RS22265 are separated by 51 nucleotides
RR42_RS22255: RR42_RS22255 - hypothetical protein, at 330,666 to 332,096
_RS22255
RR42_RS22260: RR42_RS22260 - hypothetical protein, at 332,121 to 333,455
_RS22260
RR42_RS22265: RR42_RS22265 - hypothetical protein, at 333,507 to 337,334
_RS22265
Position (kb) 332
333
334 Strain fitness (log2 ratio) -2
-1
0
1 at 331.332 kb on + strand, within RR42_RS22255 at 331.332 kb on + strand, within RR42_RS22255 at 331.332 kb on + strand, within RR42_RS22255 at 331.333 kb on - strand, within RR42_RS22255 at 331.333 kb on - strand, within RR42_RS22255 at 331.544 kb on - strand, within RR42_RS22255 at 331.544 kb on - strand, within RR42_RS22255 at 331.564 kb on + strand, within RR42_RS22255 at 331.565 kb on - strand, within RR42_RS22255 at 331.565 kb on - strand, within RR42_RS22255 at 331.631 kb on - strand, within RR42_RS22255 at 331.678 kb on + strand, within RR42_RS22255 at 331.678 kb on + strand, within RR42_RS22255 at 331.679 kb on - strand, within RR42_RS22255 at 331.681 kb on + strand, within RR42_RS22255 at 331.682 kb on - strand, within RR42_RS22255 at 331.785 kb on + strand, within RR42_RS22255 at 331.785 kb on + strand, within RR42_RS22255 at 331.933 kb on + strand, within RR42_RS22255 at 332.068 kb on - strand at 332.212 kb on + strand at 332.257 kb on + strand, within RR42_RS22260 at 332.257 kb on + strand, within RR42_RS22260 at 332.257 kb on + strand, within RR42_RS22260 at 332.258 kb on - strand, within RR42_RS22260 at 332.258 kb on - strand, within RR42_RS22260 at 332.345 kb on - strand, within RR42_RS22260 at 332.932 kb on + strand, within RR42_RS22260 at 333.015 kb on + strand, within RR42_RS22260 at 333.015 kb on + strand, within RR42_RS22260 at 333.016 kb on - strand, within RR42_RS22260 at 333.047 kb on - strand, within RR42_RS22260 at 333.418 kb on + strand at 333.489 kb on + strand at 333.489 kb on + strand at 333.489 kb on + strand at 333.490 kb on - strand at 333.528 kb on - strand at 333.540 kb on - strand at 333.965 kb on - strand, within RR42_RS22265 at 333.965 kb on - strand, within RR42_RS22265 at 333.979 kb on + strand, within RR42_RS22265 at 333.979 kb on + strand, within RR42_RS22265 at 334.177 kb on + strand, within RR42_RS22265 at 334.309 kb on - strand, within RR42_RS22265
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 331,332 + RR42_RS22255 0.47 +1.6 331,332 + RR42_RS22255 0.47 -0.1 331,332 + RR42_RS22255 0.47 -0.8 331,333 - RR42_RS22255 0.47 -0.6 331,333 - RR42_RS22255 0.47 +0.5 331,544 - RR42_RS22255 0.61 +0.4 331,544 - RR42_RS22255 0.61 -0.0 331,564 + RR42_RS22255 0.63 +0.7 331,565 - RR42_RS22255 0.63 +1.2 331,565 - RR42_RS22255 0.63 +0.1 331,631 - RR42_RS22255 0.67 +0.1 331,678 + RR42_RS22255 0.71 -0.2 331,678 + RR42_RS22255 0.71 -0.5 331,679 - RR42_RS22255 0.71 +1.0 331,681 + RR42_RS22255 0.71 +0.7 331,682 - RR42_RS22255 0.71 +0.2 331,785 + RR42_RS22255 0.78 +0.5 331,785 + RR42_RS22255 0.78 +0.0 331,933 + RR42_RS22255 0.89 -0.2 332,068 - -0.5 332,212 + +1.2 332,257 + RR42_RS22260 0.10 +0.8 332,257 + RR42_RS22260 0.10 -2.3 332,257 + RR42_RS22260 0.10 +0.1 332,258 - RR42_RS22260 0.10 -0.9 332,258 - RR42_RS22260 0.10 -0.5 332,345 - RR42_RS22260 0.17 +0.5 332,932 + RR42_RS22260 0.61 -1.4 333,015 + RR42_RS22260 0.67 -0.1 333,015 + RR42_RS22260 0.67 +0.3 333,016 - RR42_RS22260 0.67 -1.2 333,047 - RR42_RS22260 0.69 +0.5 333,418 + +0.8 333,489 + +0.5 333,489 + +0.1 333,489 + -0.8 333,490 - +0.0 333,528 - -0.1 333,540 - +0.9 333,965 - RR42_RS22265 0.12 -0.4 333,965 - RR42_RS22265 0.12 -0.9 333,979 + RR42_RS22265 0.12 +0.0 333,979 + RR42_RS22265 0.12 +0.8 334,177 + RR42_RS22265 0.18 +0.0 334,309 - RR42_RS22265 0.21 +0.2
Or see this region's nucleotide sequence